Detailed information NTG0607987

Bioinformatically predicted

Overview

• Name: pilC
• Type: Machinery gene
• Locus tag: K8O61_RS04550
• Genome accession: NZ_CP083804
• Coordinates: 1049640..1050899 (-)
• Length: 419 a.a.
• NCBI ID: WP_237656042.1
• Uniprot ID: -
• Organism: Xanthomonas cerealis pv. cerealis strain ICMP 16317
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	1051757..1071297	1049640..1050899	flank	858

Gene organization within MGE regions

Location: 1049640..1071297

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K8O61_RS04550 (K8O61_04565)	pilC	1049640..1050899 (-)	1260	WP_237656042.1	type II secretion system F family protein	Machinery gene
K8O61_RS04555 (K8O61_04570)	pilA/pilAI	1051285..1051692 (+)	408	WP_237655666.1	pilin	Machinery gene
K8O61_RS04565 (K8O61_04580)	-	1051781..1052167 (+)	387	WP_272929860.1	pilin	-
K8O61_RS04570 (K8O61_04585)	-	1052319..1054067 (+)	1749	WP_237655667.1	hypothetical protein	-
K8O61_RS04575 (K8O61_04590)	-	1054280..1055569 (+)	1290	WP_237655668.1	lipopolysaccharide biosynthesis protein	-
K8O61_RS04580 (K8O61_04595)	-	1055566..1056354 (+)	789	WP_237655669.1	class I SAM-dependent methyltransferase	-
K8O61_RS04585 (K8O61_04600)	-	1056463..1057506 (-)	1044	WP_237655670.1	glycosyltransferase family 4 protein	-
K8O61_RS04590 (K8O61_04605)	-	1057478..1058110 (-)	633	WP_237655671.1	class I SAM-dependent methyltransferase	-
K8O61_RS04595 (K8O61_04610)	-	1058221..1059327 (-)	1107	WP_237655672.1	glycosyltransferase family 4 protein	-
K8O61_RS04600 (K8O61_04615)	-	1059380..1060237 (-)	858	WP_237655673.1	glycosyltransferase family 2 protein	-
K8O61_RS04605 (K8O61_04620)	pilB	1060389..1062125 (+)	1737	WP_237655674.1	type IV-A pilus assembly ATPase PilB	Machinery gene
K8O61_RS04610 (K8O61_04625)	-	1062135..1062752 (-)	618	WP_237655675.1	Fic/DOC family protein	-
K8O61_RS04615 (K8O61_04630)	-	1062749..1062931 (-)	183	WP_003466380.1	YhfG family protein	-
K8O61_RS04620 (K8O61_04635)	pilR	1063120..1064502 (-)	1383	WP_237655676.1	sigma-54-dependent transcriptional regulator	Regulator
K8O61_RS04625 (K8O61_04640)	-	1064576..1066189 (-)	1614	WP_039008924.1	sensor histidine kinase	-
K8O61_RS04630 (K8O61_04645)	sucC	1066431..1067594 (+)	1164	WP_053833739.1	ADP-forming succinate--CoA ligase subunit beta	-
K8O61_RS04635 (K8O61_04650)	sucD	1067618..1068490 (+)	873	WP_009580520.1	succinate--CoA ligase subunit alpha	-
K8O61_RS04640 (K8O61_04655)	-	1068923..1069372 (+)	450	WP_237656120.1	hypothetical protein	-
K8O61_RS04645 (K8O61_04660)	-	1069441..1071297 (-)	1857	WP_155521865.1	hypothetical protein	-

Sequence

Protein

• Length: 419 a.a.
• Molecular weight: 45753.03 Da
• Isoelectric Point: 10.1183

>NTDB_id=607987 K8O61_RS04550 WP_237656042.1 1049640..1050899(-) (pilC) [Xanthomonas cerealis pv. cerealis strain ICMP 16317]
MSATRSAVPKEPVARNTSQQLPFVWEGTDKRGVKMKGEQTAKNANLLRAELRRQGITPSVVKPKPKPLFGSAGSKISAKD
IAFFSRQMATMMKSGVPIVGSLEIIGSGHKNPRMKKMVDQVRTDIEGGSSLYEAISKHPIQFDELYRNLVKAGEGAGVLE
TVLETVATYKENIEALKGKIKKALFYPAMVMAVALLVSSILLVWVVPQFEDVFKGFGAELPAFTQMIVAASRFMVAYWWM
LLLVLIGTIVGFVFAYKRSPSMQHGMDRLILKVPIIGQIMHNSSVARFSRTLGVTFRAGVPLVEALDIVAGATGNSVYEK
AVLRMRDDVSVGYPVNVAMKQTNLFPHMVIQMTAIGEEAGALDAMLFKVAEYFEQEVNNAVDALSSLIEPLIMVFIGTIV
GGMVIGMYLPIFKLASVVG

Nucleotide

• Length: 1260 bp

>NTDB_id=607987 K8O61_RS04550 WP_237656042.1 1049640..1050899(-) (pilC) [Xanthomonas cerealis pv. cerealis strain ICMP 16317]
ATGTCCGCAACTCGTAGCGCAGTACCCAAAGAGCCCGTCGCGCGCAATACCAGCCAGCAGCTGCCGTTCGTCTGGGAAGG
GACGGACAAGCGCGGCGTCAAGATGAAGGGCGAGCAGACCGCCAAGAACGCCAACCTGCTGCGCGCCGAGCTGCGCCGCC
AGGGCATCACGCCGTCGGTGGTCAAGCCCAAGCCCAAGCCGTTGTTCGGCTCTGCCGGCAGCAAGATCAGCGCGAAGGAC
ATCGCTTTTTTCAGCCGCCAGATGGCGACCATGATGAAGTCGGGCGTCCCCATCGTGGGATCGCTGGAGATCATCGGCAG
CGGGCACAAGAACCCGCGCATGAAGAAGATGGTGGATCAGGTCCGCACCGACATCGAGGGTGGCTCGTCGCTCTATGAAG
CGATCAGCAAGCACCCGATTCAGTTCGACGAGCTTTACCGCAATCTGGTCAAGGCCGGCGAAGGCGCCGGCGTGCTGGAG
ACCGTGCTGGAAACGGTTGCGACCTACAAGGAAAACATCGAGGCGCTGAAGGGCAAGATCAAGAAGGCCTTGTTCTACCC
GGCCATGGTCATGGCGGTCGCTCTGCTGGTCAGTTCGATCCTGCTGGTGTGGGTGGTGCCACAGTTCGAGGATGTGTTCA
AGGGCTTTGGCGCGGAGTTGCCTGCGTTTACGCAGATGATCGTGGCCGCATCGCGCTTCATGGTCGCCTATTGGTGGATG
CTGTTGCTGGTGCTGATAGGCACCATCGTCGGCTTCGTCTTCGCCTACAAACGCTCGCCCTCAATGCAGCACGGCATGGA
CCGGCTGATCCTGAAGGTGCCGATCATCGGCCAGATCATGCACAACAGCTCCGTGGCCCGCTTTTCGCGCACATTGGGCG
TGACCTTCCGCGCTGGCGTCCCGTTGGTGGAGGCCCTGGACATCGTCGCCGGCGCCACCGGCAACAGCGTCTACGAGAAA
GCCGTGCTGCGCATGCGCGACGACGTCTCGGTGGGCTACCCGGTCAACGTGGCGATGAAGCAGACCAACCTGTTTCCGCA
CATGGTCATCCAGATGACCGCCATCGGCGAAGAGGCAGGCGCACTGGATGCGATGCTGTTCAAGGTGGCCGAGTACTTCG
AGCAGGAAGTGAACAATGCGGTGGATGCGTTGAGCAGCCTGATCGAGCCGCTGATCATGGTGTTCATTGGTACCATCGTC
GGCGGCATGGTGATCGGCATGTATCTGCCCATCTTCAAGCTGGCCTCCGTCGTTGGATAA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein F (T2SSF) (288-410)

• Type II secretion system (T2SS), protein F (T2SSF) (84-207)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilC	Pseudomonas stutzeri DSM 10701	53.788	94.511	0.508
pilC	Legionella pneumophila strain ERS1305867	51.733	96.42	0.499
pilC	Acinetobacter baylyi ADP1	50.98	97.375	0.496
pilC	Acinetobacter baumannii D1279779	50.49	97.375	0.492
pilG	Neisseria gonorrhoeae MS11	42.965	94.988	0.408
pilC	Vibrio cholerae strain A1552	42.222	96.659	0.408
pilG	Neisseria meningitidis 44/76-A	42.356	95.227	0.403
pilC	Vibrio campbellii strain DS40M4	40.05	94.749	0.379
pilC	Thermus thermophilus HB27	39.25	95.465	0.375