Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   K6748_RS01580 Genome accession   NZ_CP082307
Coordinates   335160..336905 (+) Length   581 a.a.
NCBI ID   WP_238958948.1    Uniprot ID   -
Organism   Vibrio alginolyticus strain HNV2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 330160..341905
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6748_RS01560 (K6748_01560) pilM 332468..333484 (+) 1017 WP_005381324.1 type IV pilus assembly protein PilM Machinery gene
  K6748_RS01565 (K6748_01565) pilN 333468..334043 (+) 576 WP_005381323.1 PilN domain-containing protein Machinery gene
  K6748_RS01570 (K6748_01570) pilO 334036..334629 (+) 594 WP_053350733.1 type 4a pilus biogenesis protein PilO Machinery gene
  K6748_RS01575 (K6748_01575) pilP 334619..335134 (+) 516 WP_205461596.1 pilus assembly protein PilP Machinery gene
  K6748_RS01580 (K6748_01580) pilQ 335160..336905 (+) 1746 WP_238958948.1 type IV pilus secretin PilQ family protein Machinery gene
  K6748_RS01585 (K6748_01585) aroK 337093..337611 (+) 519 WP_005381319.1 shikimate kinase AroK -
  K6748_RS01590 (K6748_01590) aroB 337644..338744 (+) 1101 WP_005381318.1 3-dehydroquinate synthase -
  K6748_RS01595 (K6748_01595) - 338797..340308 (+) 1512 WP_205461597.1 AAA family ATPase -
  K6748_RS01600 (K6748_01600) - 340378..341217 (+) 840 WP_005382762.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 581 a.a.        Molecular weight: 63647.67 Da        Isoelectric Point: 4.4533

>NTDB_id=601175 K6748_RS01580 WP_238958948.1 335160..336905(+) (pilQ) [Vibrio alginolyticus strain HNV2]
MKMRQGLTRTPRILTGLFSLWLFVFLAPLAAAEEVSSNALKSIDFRTNKDKDAVIVVELASPAAIVDIKRVQEGLSIDLL
NTSVKDEQLYLLDVKDFSTVVESVEVFRDTSTTRLVAHINEEYMHDYRLTGPFLEIKVAKIKPNEKAPDKSILEREGKLI
SINFQDIPVRNVLQLIADYNQFNLVVSDSVEGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPTDELDLREKQQ
LEKQKLTEEMGELSSEIIKVNFAKASDIAEMINGEGNISMLSERGSMTIDERTNSLLIRELPENIEVIREIIESLDIPVK
QVQIEARIVTINEGNMDELGIRWGFTSINGSNTVGGSIENNLATIGLYNGEGGDDGGDGDGVGINDFLNVNLAATSPNAT
SIAFQVAKLGSDTLLDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATTVTFKKAVLSLKVTPQI
TPDNRLVLDLSVTQDRPGQVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPLLGALFRR
SYENVGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1746 bp        

>NTDB_id=601175 K6748_RS01580 WP_238958948.1 335160..336905(+) (pilQ) [Vibrio alginolyticus strain HNV2]
ATGAAAATGAGACAAGGATTAACACGTACACCAAGGATACTTACTGGGCTCTTCTCCTTATGGTTGTTTGTATTTTTAGC
CCCTTTAGCGGCAGCTGAAGAAGTTTCGTCCAATGCCCTAAAAAGTATCGACTTTCGAACTAATAAAGATAAAGACGCCG
TGATTGTCGTCGAATTGGCGTCACCGGCCGCGATTGTAGATATCAAGCGTGTACAGGAAGGGTTAAGTATCGATTTGCTT
AACACTTCAGTAAAAGACGAGCAATTGTATCTCCTAGACGTGAAAGACTTTTCGACTGTGGTTGAGAGCGTAGAAGTGTT
TCGCGACACCTCGACGACGCGCTTGGTTGCGCACATCAATGAAGAATACATGCATGATTACCGTTTAACGGGGCCTTTTT
TGGAAATCAAAGTAGCGAAGATCAAACCCAATGAGAAAGCCCCAGATAAAAGCATTTTAGAAAGAGAAGGTAAGCTTATT
TCGATTAACTTCCAAGATATTCCTGTGCGAAACGTCTTGCAGCTTATTGCTGATTACAACCAATTCAATTTGGTTGTCTC
CGACTCTGTAGAAGGCAACCTCACGCTTCGTCTTGATGGTGTCCCTTGGCAGCAAGTGCTGGATATTATTTTGCAAGTTA
AGGGGTTAGATAAGCGTGTTGATGGCAACGTGATTTTAGTTGCTCCGACTGATGAGTTGGACTTGCGTGAGAAGCAGCAG
TTAGAAAAACAAAAACTTACGGAAGAAATGGGAGAGCTTTCATCTGAAATCATCAAAGTTAATTTTGCTAAAGCATCAGA
CATTGCGGAGATGATCAATGGAGAAGGAAATATCAGCATGCTGTCAGAGCGCGGCAGTATGACCATTGATGAGCGAACTA
ACTCGTTATTGATTAGAGAGTTACCTGAAAACATCGAGGTGATTCGAGAAATTATTGAGTCTTTGGATATTCCAGTTAAA
CAGGTTCAAATTGAGGCGCGTATTGTGACCATCAATGAAGGTAACATGGACGAGTTAGGGATTCGTTGGGGTTTTACGTC
TATTAATGGCAGTAATACAGTGGGCGGTTCTATTGAGAACAACCTCGCCACGATCGGTCTCTATAATGGAGAAGGGGGTG
ATGACGGTGGTGACGGTGATGGAGTAGGGATCAATGACTTCCTAAATGTAAACTTAGCTGCAACCAGTCCGAATGCCACC
AGCATTGCTTTTCAGGTCGCAAAGTTAGGTTCAGACACCTTGTTGGATCTTGAGCTTTCTGCTCTGCAACAAGAGTCTAA
AGCTGAGATTATCTCGAGCCCACGTTTGATCACTACCAATAAGAAACCTGCCTATATCGAACAAGGTACAGAGATTCCTT
ATCTTGAGTCATCATCAAGTGGTGCAACGACGGTGACGTTCAAAAAGGCGGTATTAAGTTTGAAGGTAACCCCGCAAATC
ACACCGGATAACCGCTTAGTATTGGATTTAAGTGTCACGCAAGATAGACCTGGACAAGTCGTGAAGACAGGCACTGGTGA
AGCTGTTGCGATTGATACTCAACGTATCGGGACGCAAGTGTTAGTGAATAACGGTGAAACGGTCGTTCTTGGTGGTATTT
TTCAGCACAGCATTACTAACTCGGTGGACAAAGTGCCTTTGTTGGGCGATCTTCCGCTATTAGGTGCGCTTTTTCGTCGA
AGTTATGAAAATGTTGGTAAAAGTGAGCTGTTAATTTTCGTCACGCCTAAGGTGGTGATTCAGTAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(256-321)

Bacterial type II and III secretion system protein (Secretin)

(421-579)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

92.599

100

0.926
  pilQ Vibrio cholerae strain A1552

74.57

100

0.747
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

74.57

100

0.747
  pilQ Pseudomonas aeruginosa PAK

39.415

100

0.394
  pilQ Legionella pneumophila strain Lp02

40.608

96.213

0.391
  pilQ Legionella pneumophila strain ERS1305867

40.608

96.213

0.391
  comQ Acinetobacter baylyi ADP1

37.414

99.828

0.373
  pilQ Acinetobacter baumannii D1279779

35.953

100

0.37
  pilQ Acinetobacter baumannii strain A118

35.953

100

0.37