Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   K6748_RS01570 Genome accession   NZ_CP082307
Coordinates   334036..334629 (+) Length   197 a.a.
NCBI ID   WP_053350733.1    Uniprot ID   -
Organism   Vibrio alginolyticus strain HNV2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 329036..339629
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6748_RS01555 (K6748_01555) - 329762..332323 (-) 2562 WP_206958471.1 PBP1A family penicillin-binding protein -
  K6748_RS01560 (K6748_01560) pilM 332468..333484 (+) 1017 WP_005381324.1 type IV pilus assembly protein PilM Machinery gene
  K6748_RS01565 (K6748_01565) pilN 333468..334043 (+) 576 WP_005381323.1 PilN domain-containing protein Machinery gene
  K6748_RS01570 (K6748_01570) pilO 334036..334629 (+) 594 WP_053350733.1 type 4a pilus biogenesis protein PilO Machinery gene
  K6748_RS01575 (K6748_01575) pilP 334619..335134 (+) 516 WP_205461596.1 pilus assembly protein PilP Machinery gene
  K6748_RS01580 (K6748_01580) pilQ 335160..336905 (+) 1746 WP_238958948.1 type IV pilus secretin PilQ family protein Machinery gene
  K6748_RS01585 (K6748_01585) aroK 337093..337611 (+) 519 WP_005381319.1 shikimate kinase AroK -
  K6748_RS01590 (K6748_01590) aroB 337644..338744 (+) 1101 WP_005381318.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23029.56 Da        Isoelectric Point: 5.0948

>NTDB_id=601173 K6748_RS01570 WP_053350733.1 334036..334629(+) (pilO) [Vibrio alginolyticus strain HNV2]
MVDWQDLEIDEMAEWPLLPQIVVIALLIMVIQGLGYWFYLKPKEEQLNHLIQQEQTLKVALKIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGEKQSEHFLYRLPLNIELTGDFHDIGHFSQAIATLPRII
TFKDVTWQRVSQESETLHFRVRANTYQFKPEETKNDK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=601173 K6748_RS01570 WP_053350733.1 334036..334629(+) (pilO) [Vibrio alginolyticus strain HNV2]
GTGGTTGATTGGCAGGATCTCGAAATAGATGAAATGGCCGAGTGGCCATTGTTGCCCCAAATTGTGGTAATCGCGCTTCT
GATCATGGTGATTCAAGGCTTAGGTTATTGGTTTTACCTTAAACCGAAAGAAGAACAGTTGAATCACTTGATCCAGCAGG
AGCAGACACTCAAGGTGGCATTGAAGATCAAAGCGAACAAAGTGGCAGCGTTGCCTCAGCTTCAATCTCAATTGGATGAA
CTTACCACTCGTTACGAGTTATTACTGCAACAACTGCCTGCGCAAAAAGAGCTGGCTACAATGCTCGCTTCAGTGAATGA
GCTTGGCATCGACAATAAGCTGACTTTCACCCGAATCGACTGGGGCGAAAAACAATCAGAGCACTTCCTTTATCGTCTCC
CTTTAAACATTGAGTTAACGGGCGACTTTCATGACATCGGCCATTTTTCTCAGGCGATAGCGACGCTACCTCGAATCATC
ACGTTTAAAGATGTGACTTGGCAACGTGTCAGCCAAGAGAGCGAAACTCTGCACTTTCGGGTGCGCGCTAACACCTATCA
GTTTAAGCCGGAGGAGACGAAAAATGACAAGTAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(48-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

87.895

96.447

0.848
  pilO Vibrio cholerae strain A1552

70.681

96.954

0.685