NTDB

Overview

Name	comEA	Type	Machinery gene
Locus tag	M5C73_RS09700	Genome accession	NZ_CP098790
Coordinates	1972188..1972835 (-)	Length	215 a.a.
NCBI ID	WP_058203544.1	Uniprot ID	-
Organism	Lactococcus lactis strain WiKim0124
Function	dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1967188..1977835

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M5C73_RS09675 (M5C73_09675)	atpF	1967257..1967763 (-)	507	WP_010906128.1	F0F1 ATP synthase subunit B	-
M5C73_RS09680 (M5C73_09680)	atpB	1967778..1968491 (-)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
M5C73_RS09685 (M5C73_09685)	-	1968536..1968751 (-)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
M5C73_RS09690 (M5C73_09690)	-	1968936..1969712 (-)	777	WP_004255244.1	alpha/beta hydrolase family protein	-
M5C73_RS09695 (M5C73_09695)	comEC	1969997..1972207 (-)	2211	WP_252160076.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
M5C73_RS09700 (M5C73_09700)	comEA	1972188..1972835 (-)	648	WP_058203544.1	ComEA family DNA-binding protein	Machinery gene
M5C73_RS09705 (M5C73_09705)	-	1972895..1974256 (-)	1362	WP_237579516.1	ABC transporter permease	-
M5C73_RS09710 (M5C73_09710)	-	1974253..1975185 (-)	933	WP_004255238.1	ABC transporter ATP-binding protein	-
M5C73_RS09715 (M5C73_09715)	-	1975290..1975688 (-)	399	WP_004255236.1	hypothetical protein	-
M5C73_RS09720 (M5C73_09720)	-	1975792..1976355 (-)	564	WP_004255233.1	GNAT family N-acetyltransferase	-
M5C73_RS09725 (M5C73_09725)	-	1976528..1977706 (-)	1179	WP_012898345.1	SLC13 family permease	-

Sequence

Protein

Download Length: 215 a.a. Molecular weight: 23634.80 Da Isoelectric Point: 4.8659

                                >NTDB_id=601174 M5C73_RS09700 WP_058203544.1 1972188..1972835(-) (comEA) [Lactococcus lactis strain WiKim0124]

MDKILEKVKEYWKMIVLIVCGLIAGGIFYVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP

NVYQISPDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSTTTEKININQAD

VTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide

Download Length: 648 bp

                                >NTDB_id=601174 M5C73_RS09700 WP_058203544.1 1972188..1972835(-) (comEA) [Lactococcus lactis strain WiKim0124]

ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAATTGTTTGTGGGCTCATTGCTGGTGGGAT

TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT

CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT

AATGTCTATCAAATTTCGCCAGATGAACGTCTAGTTGATTTAATTAAGGAAGCTGGTGGTTTTACTGACCAAGCTGACCA

GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG

AAAATACTGACAGCTCTGCTAATTCTGTCACTAGTCAAGTCTCAACAACCACTGAAAAAATAAATATCAATCAAGCAGAT

GTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATTGATTTTCGGACGAAAAATGGTGA

TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT

TTGAATAA

Domains

Predicted by InterProScan.

Helix-hairpin-helix motif (HHH_3)

(151-212)

SLBB domain (SLBB)

(70-125)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comEA	Lactococcus lactis subsp. cremoris KW2	74.771	100	0.758
comEA/celA/cilE	Streptococcus mitis SK321	44.444	100	0.447
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	43.119	100	0.437
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.096	100	0.419
comEA/celA/cilE	Streptococcus pneumoniae D39	41.096	100	0.419
comEA/celA/cilE	Streptococcus pneumoniae R6	41.096	100	0.419
comEA/celA/cilE	Streptococcus mitis NCTC 12261	41.284	100	0.419
comEA	Latilactobacillus sakei subsp. sakei 23K	36.123	100	0.381

Detailed information NTG0601174