Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   K5H97_RS27515 Genome accession   NZ_CP081966
Coordinates   5911897..5913102 (-) Length   401 a.a.
NCBI ID   WP_088852014.1    Uniprot ID   -
Organism   Pseudomonas mosselii strain DSM 17497     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5906897..5918102
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5H97_RS27500 (K5H97_27500) - 5908546..5910165 (-) 1620 WP_028689107.1 AAA family ATPase -
  K5H97_RS27505 (K5H97_27505) aroB 5910181..5911278 (-) 1098 WP_028689108.1 3-dehydroquinate synthase -
  K5H97_RS27510 (K5H97_27510) aroK 5911374..5911892 (-) 519 WP_028689109.1 shikimate kinase AroK -
  K5H97_RS27515 (K5H97_27515) pilQ 5911897..5913102 (-) 1206 WP_088852014.1 type IV pilus secretin PilQ Machinery gene
  K5H97_RS27520 (K5H97_27520) - 5913059..5914072 (-) 1014 WP_051555621.1 pilus assembly protein PilP -
  K5H97_RS27525 (K5H97_27525) - 5914069..5914608 (-) 540 WP_028689111.1 PilN domain-containing protein -
  K5H97_RS27530 (K5H97_27530) pilM 5914605..5915501 (-) 897 WP_028689112.1 type IV pilus biogenesis protein PilM -

Sequence

Protein


Download         Length: 401 a.a.        Molecular weight: 43437.87 Da        Isoelectric Point: 4.9947

>NTDB_id=598689 K5H97_RS27515 WP_088852014.1 5911897..5913102(-) (pilQ) [Pseudomonas mosselii strain DSM 17497]
MLGLQATQAETFQGEPMSLNLQDVELRSVLQVMADHAGVNLVVGDSVQGRITLRLEDVPWEQALDLVLRSKGLGRRREGN
VLLVAPVAELAGQVQGIEPLQRELIPVFHAKAGELAELLLASLADDGALAGRGSLSVDARTNTLVATQPAARLAELRRLV
TQLDVPVRQVMIEARIVEANVDYEKALGVRWGGPLYGSDRRLGSDLFVDLGVERGGAALGVGLLRSDILLDLELSAMEKS
GNGEIISQPKVVTADKETARILKGTEVPYQETTRSGATSVSFREASLSLEVTPQITPDGKVIMAVRVTKDEPDFINALND
VPPIRKNEVNAKVRVTDGQTIVIGGVYSTSQNKVVDKVPFFGDLPYVGRLFRRDALQEKKSELLVFLTPRIMSDQAIAVS
R

Nucleotide


Download         Length: 1206 bp        

>NTDB_id=598689 K5H97_RS27515 WP_088852014.1 5911897..5913102(-) (pilQ) [Pseudomonas mosselii strain DSM 17497]
ATGTTGGGGCTTCAGGCGACGCAGGCCGAGACGTTTCAGGGTGAGCCGATGTCGCTCAACCTCCAGGACGTCGAGCTGCG
TTCGGTGCTTCAGGTCATGGCCGATCACGCGGGCGTCAACCTGGTGGTCGGCGACTCGGTGCAGGGACGGATTACCCTGC
GCCTGGAAGACGTGCCCTGGGAGCAGGCGCTTGATCTGGTGCTGCGCAGCAAGGGCTTGGGCAGGCGCCGGGAGGGGAAT
GTGCTGCTGGTTGCTCCTGTGGCAGAGCTCGCTGGGCAGGTCCAGGGCATCGAGCCACTGCAGCGTGAATTGATCCCTGT
CTTCCATGCCAAGGCCGGCGAGCTGGCGGAGCTGCTGCTGGCGAGCCTGGCGGACGACGGTGCCCTGGCCGGGCGTGGCA
GCCTCAGTGTGGACGCTCGCACCAATACCCTGGTAGCCACCCAGCCTGCCGCGCGGCTCGCCGAACTGCGCAGGCTGGTC
ACTCAGCTGGATGTGCCGGTGCGCCAGGTCATGATCGAGGCACGGATCGTCGAGGCCAATGTCGATTACGAAAAGGCCCT
GGGCGTGCGCTGGGGTGGACCGCTGTATGGCTCGGACCGCCGGCTGGGCAGCGACCTTTTCGTCGATCTTGGCGTCGAGC
GGGGAGGTGCCGCGTTGGGAGTGGGGCTGCTGCGCAGCGATATCCTGCTCGATCTGGAGCTTAGCGCCATGGAAAAAAGC
GGCAATGGCGAGATCATCTCCCAACCCAAGGTGGTCACGGCCGACAAGGAAACCGCGCGCATACTCAAGGGCACCGAAGT
GCCCTACCAGGAAACCACCCGCAGTGGCGCCACTTCGGTCTCCTTCCGAGAGGCGTCCCTGTCGCTGGAGGTGACGCCGC
AGATCACGCCGGACGGCAAGGTGATCATGGCGGTGCGGGTGACCAAGGACGAGCCGGACTTCATCAATGCCCTTAACGAT
GTGCCGCCGATCCGCAAGAACGAGGTCAACGCCAAGGTGCGGGTCACCGATGGGCAGACCATTGTGATTGGTGGCGTGTA
CTCGACGTCGCAGAACAAAGTGGTCGACAAGGTGCCATTTTTTGGCGATCTGCCGTATGTTGGGCGGCTGTTTCGACGCG
ATGCATTACAAGAGAAAAAATCCGAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGGCGATTGCTGTGAGT
CGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(39-86)

Bacterial type II/III secretion system short domain (Secretin_N)

(104-169)

Bacterial type II and III secretion system protein (Secretin)

(236-392)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

57.11

100

0.621
  comQ Acinetobacter baylyi ADP1

42.163

100

0.476
  pilQ Acinetobacter baumannii D1279779

41.087

100

0.471
  pilQ Acinetobacter baumannii strain A118

41.087

100

0.471
  pilQ Legionella pneumophila strain ERS1305867

43.365

100

0.456
  pilQ Legionella pneumophila strain Lp02

43.365

100

0.456
  pilQ Vibrio campbellii strain DS40M4

40.094

100

0.424
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

40.144

100

0.416
  pilQ Vibrio cholerae strain A1552

40.144

100

0.416
  pilQ Neisseria meningitidis M1080

35.461

100

0.374
  pilQ Neisseria gonorrhoeae strain FA1090

35.071

100

0.369
  pilQ Neisseria meningitidis MC58

34.834

100

0.367