Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC/cglC   Type   Machinery gene
Locus tag   K2228_RS09030 Genome accession   NZ_CP080759
Coordinates   1805206..1805481 (-) Length   91 a.a.
NCBI ID   WP_002356991.1    Uniprot ID   A0A1B4XPV0
Organism   Enterococcus faecalis strain M817     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1761700..1805182 1805206..1805481 flank 24


Gene organization within MGE regions


Location: 1761700..1805481
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K2228_RS08715 (K2228_08675) - 1761700..1762707 (-) 1008 WP_002357063.1 class I SAM-dependent methyltransferase -
  K2228_RS08720 (K2228_08680) comGG 1762836..1763189 (-) 354 WP_002362054.1 competence type IV pilus minor pilin ComGG -
  K2228_RS08725 (K2228_08685) comGF 1763189..1763623 (-) 435 WP_002378441.1 competence type IV pilus minor pilin ComGF -
  K2228_RS08730 (K2228_08690) - 1763613..1764014 (-) 402 WP_002410542.1 type II secretion system protein -
  K2228_RS14410 - 1764318..1764443 (+) 126 WP_002364184.1 hypothetical protein -
  K2228_RS08735 (K2228_08695) hemH 1764741..1765682 (-) 942 WP_116491831.1 ferrochelatase -
  K2228_RS08745 (K2228_08705) - 1766004..1766825 (-) 822 WP_114232967.1 DUF3800 domain-containing protein -
  K2228_RS08750 (K2228_08710) - 1767450..1768751 (-) 1302 WP_114232968.1 LysM peptidoglycan-binding domain-containing protein -
  K2228_RS08755 (K2228_08715) - 1768754..1768960 (-) 207 WP_114232969.1 phage holin -
  K2228_RS08760 (K2228_08720) - 1768957..1769178 (-) 222 WP_002397443.1 hypothetical protein -
  K2228_RS08765 (K2228_08725) - 1769206..1769361 (-) 156 WP_002364192.1 XkdX family protein -
  K2228_RS08770 (K2228_08730) - 1769363..1769683 (-) 321 WP_002388445.1 hypothetical protein -
  K2228_RS08775 (K2228_08735) - 1769697..1770188 (-) 492 WP_010711694.1 hypothetical protein -
  K2228_RS08780 (K2228_08740) - 1770188..1770466 (-) 279 WP_116491832.1 hypothetical protein -
  K2228_RS08785 (K2228_08745) - 1770463..1771059 (-) 597 WP_116491833.1 hypothetical protein -
  K2228_RS08790 (K2228_08750) - 1771052..1771933 (-) 882 WP_116491834.1 phage baseplate upper protein -
  K2228_RS08795 (K2228_08755) - 1771952..1774759 (-) 2808 WP_164549299.1 phage tail spike protein -
  K2228_RS08800 (K2228_08760) - 1774741..1775475 (-) 735 WP_104670712.1 phage tail protein -
  K2228_RS08805 (K2228_08765) - 1775465..1778362 (-) 2898 WP_164549302.1 tape measure protein -
  K2228_RS08810 (K2228_08770) - 1778610..1778960 (-) 351 WP_010820912.1 hypothetical protein -
  K2228_RS08815 (K2228_08775) - 1779012..1779860 (-) 849 WP_002387287.1 major tail protein -
  K2228_RS08820 (K2228_08780) - 1779861..1780235 (-) 375 WP_116491837.1 DUF6838 family protein -
  K2228_RS08825 (K2228_08785) - 1780238..1780636 (-) 399 WP_002357036.1 HK97 gp10 family phage protein -
  K2228_RS08830 (K2228_08790) - 1780629..1781003 (-) 375 WP_002387285.1 hypothetical protein -
  K2228_RS08835 (K2228_08795) - 1781000..1781344 (-) 345 WP_002387282.1 hypothetical protein -
  K2228_RS08840 (K2228_08800) - 1781358..1781540 (-) 183 WP_002387281.1 hypothetical protein -
  K2228_RS08845 (K2228_08805) - 1781569..1782456 (-) 888 WP_002387280.1 DUF5309 domain-containing protein -
  K2228_RS08850 (K2228_08810) - 1782470..1783093 (-) 624 WP_010706493.1 DUF4355 domain-containing protein -
  K2228_RS08855 (K2228_08815) - 1783312..1783632 (-) 321 WP_116491838.1 hypothetical protein -
  K2228_RS08860 (K2228_08820) - 1783689..1783919 (-) 231 WP_002380437.1 hypothetical protein -
  K2228_RS08865 (K2228_08825) - 1783920..1785821 (-) 1902 WP_116491839.1 head protein -
  K2228_RS08870 (K2228_08830) - 1785799..1787271 (-) 1473 WP_116491840.1 phage portal protein -
  K2228_RS08875 (K2228_08835) terL 1787283..1788671 (-) 1389 WP_164549303.1 phage terminase large subunit -
  K2228_RS08880 (K2228_08840) - 1788664..1789125 (-) 462 WP_002399680.1 helix-turn-helix domain-containing protein -
  K2228_RS08885 (K2228_08845) - 1789156..1789386 (-) 231 WP_114232998.1 hypothetical protein -
  K2228_RS08895 (K2228_08855) - 1790227..1790643 (-) 417 WP_116491841.1 ArpU family phage packaging/lysis transcriptional regulator -
  K2228_RS08900 (K2228_08860) - 1791017..1791223 (-) 207 WP_116491842.1 hypothetical protein -
  K2228_RS08905 (K2228_08865) - 1791237..1791821 (-) 585 WP_010711679.1 DUF3850 domain-containing protein -
  K2228_RS08910 (K2228_08870) - 1791833..1792081 (-) 249 WP_002415426.1 hypothetical protein -
  K2228_RS08915 (K2228_08875) - 1792078..1792431 (-) 354 WP_002415427.1 hypothetical protein -
  K2228_RS08920 (K2228_08880) - 1792581..1793339 (-) 759 WP_025191955.1 N-6 DNA methylase -
  K2228_RS08925 (K2228_08885) - 1793392..1793562 (-) 171 WP_033603020.1 hypothetical protein -
  K2228_RS08930 (K2228_08890) - 1793626..1794054 (-) 429 WP_164549300.1 RusA family crossover junction endodeoxyribonuclease -
  K2228_RS08935 (K2228_08895) - 1794051..1795004 (-) 954 WP_116491844.1 phage replisome organizer N-terminal domain-containing protein -
  K2228_RS08940 (K2228_08900) - 1795008..1795688 (-) 681 WP_116491845.1 putative HNHc nuclease -
  K2228_RS08945 (K2228_08905) - 1795685..1796623 (-) 939 WP_116491846.1 DUF1351 domain-containing protein -
  K2228_RS08950 (K2228_08910) - 1796608..1797285 (-) 678 WP_116491847.1 ERF family protein -
  K2228_RS08955 (K2228_08915) - 1797278..1797523 (-) 246 WP_116491848.1 hypothetical protein -
  K2228_RS08960 (K2228_08920) - 1797703..1798026 (-) 324 WP_002368201.1 hypothetical protein -
  K2228_RS08965 (K2228_08925) - 1798099..1798842 (-) 744 WP_002368200.1 hypothetical protein -
  K2228_RS08970 (K2228_08930) - 1798855..1799262 (-) 408 WP_002363338.1 hypothetical protein -
  K2228_RS08975 (K2228_08935) - 1799331..1799531 (-) 201 WP_116491849.1 hypothetical protein -
  K2228_RS08980 (K2228_08940) - 1799542..1799727 (-) 186 WP_099704312.1 hypothetical protein -
  K2228_RS08985 (K2228_08945) - 1799738..1800450 (-) 713 Protein_1750 Rha family transcriptional regulator -
  K2228_RS08990 (K2228_08950) - 1800489..1800806 (-) 318 WP_002356999.1 hypothetical protein -
  K2228_RS08995 (K2228_08955) - 1800812..1801003 (-) 192 WP_002356998.1 hypothetical protein -
  K2228_RS09000 (K2228_08960) - 1801296..1801637 (+) 342 WP_002396615.1 helix-turn-helix transcriptional regulator -
  K2228_RS09005 (K2228_08965) - 1801642..1802289 (+) 648 WP_002372050.1 ImmA/IrrE family metallo-endopeptidase -
  K2228_RS09010 (K2228_08970) - 1802407..1803171 (+) 765 WP_229077509.1 LysM domain-containing protein -
  K2228_RS09015 (K2228_08975) - 1803186..1803515 (+) 330 WP_002369911.1 hypothetical protein -
  K2228_RS09020 (K2228_08980) - 1803590..1804738 (+) 1149 WP_116491852.1 site-specific integrase -
  K2228_RS09025 (K2228_08985) comGD 1804766..1805209 (-) 444 WP_116491816.1 competence type IV pilus minor pilin ComGD -
  K2228_RS09030 (K2228_08990) comGC/cglC 1805206..1805481 (-) 276 WP_002356991.1 competence type IV pilus major pilin ComGC Machinery gene

Sequence

Protein


Download         Length: 91 a.a.        Molecular weight: 10464.41 Da        Isoelectric Point: 9.3192

>NTDB_id=594685 K2228_RS09030 WP_002356991.1 1805206..1805481(-) (comGC/cglC) [Enterococcus faecalis strain M817]
MKKKQKYAGFTLLEMLIVLLIISVLILLFVPNLAKHKETVDKKGNEAIVKIVESQIELYTLEKNKTPSLNELVNEGYITK
EQLDKYTAEKQ

Nucleotide


Download         Length: 276 bp        

>NTDB_id=594685 K2228_RS09030 WP_002356991.1 1805206..1805481(-) (comGC/cglC) [Enterococcus faecalis strain M817]
ATGAAAAAGAAACAAAAATACGCAGGGTTTACATTATTAGAAATGTTGATTGTCTTATTGATTATTTCCGTATTGATTTT
ACTTTTTGTTCCTAACTTAGCGAAACATAAAGAAACAGTTGATAAAAAAGGCAATGAAGCAATCGTAAAAATTGTAGAAT
CACAAATCGAGCTCTACACACTAGAAAAAAATAAGACGCCTTCCTTAAATGAATTAGTCAACGAAGGCTACATTACTAAA
GAGCAGTTAGATAAATATACAGCAGAAAAGCAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(5-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B4XPV0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC/cglC Streptococcus pneumoniae Rx1

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae D39

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae R6

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae TIGR4

63.095

92.308

0.582
  comGC/cglC Streptococcus mitis NCTC 12261

61.905

92.308

0.571
  comGC/cglC Streptococcus mitis SK321

61.176

93.407

0.571
  comGC Lactococcus lactis subsp. cremoris KW2

58.14

94.505

0.549
  comYC Streptococcus gordonii str. Challis substr. CH1

56.322

95.604

0.538
  comYC Streptococcus suis isolate S10

52.326

94.505

0.495
  comYC Streptococcus mutans UA159

57.692

85.714

0.495
  comYC Streptococcus mutans UA140

57.692

85.714

0.495
  comGC Latilactobacillus sakei subsp. sakei 23K

46.154

100

0.462
  comGC Staphylococcus aureus MW2

46.835

86.813

0.407
  comGC Staphylococcus aureus N315

46.835

86.813

0.407
  comGC Bacillus subtilis subsp. subtilis str. 168

48.649

81.319

0.396