Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   K1X80_RS03015 Genome accession   NZ_CP080494
Coordinates   681819..683150 (+) Length   443 a.a.
NCBI ID   WP_220172948.1    Uniprot ID   -
Organism   Pseudomonas sp. So3.2b     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 676819..688150
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K1X80_RS02995 (K1X80_02995) pilM 679416..680069 (+) 654 WP_057958078.1 type IV pilus biogenesis protein PilM -
  K1X80_RS03000 (K1X80_03000) - 680069..680620 (+) 552 WP_057958079.1 PilN domain-containing protein -
  K1X80_RS03005 (K1X80_03005) - 680617..681237 (+) 621 WP_057958080.1 type 4a pilus biogenesis protein PilO -
  K1X80_RS03010 (K1X80_03010) - 681366..681749 (+) 384 Protein_601 pilus assembly protein PilP -
  K1X80_RS03015 (K1X80_03015) pilQ 681819..683150 (+) 1332 WP_220172948.1 type IV pilus secretin PilQ Machinery gene
  K1X80_RS03020 (K1X80_03020) aroK 683155..683673 (+) 519 WP_057437234.1 shikimate kinase AroK -
  K1X80_RS03025 (K1X80_03025) aroB 683821..684921 (+) 1101 WP_057958082.1 3-dehydroquinate synthase -
  K1X80_RS03030 (K1X80_03030) - 684932..686506 (+) 1575 WP_120731330.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 443 a.a.        Molecular weight: 48233.10 Da        Isoelectric Point: 4.9684

>NTDB_id=593244 K1X80_RS03015 WP_220172948.1 681819..683150(+) (pilQ) [Pseudomonas sp. So3.2b]
MTEAVPKRIDLIQLPPPGSSPLNTARGPFTGNRLSLNFQNIEVRAVLQQIADVAGLNLVAGDDVQGSITLRLKDVPWDQA
LDLVLQAKGLDRRVTANVLLVAPAEELAARELLALESHKLMTELTPLRRELLQVNYAKASELAKLFQSVTGLEGATDERG
SVTVDDRTNNIIAHQTGERLEELRRIVSQLDVPVRQVMIEARIVEANVDYDKSVGVRWGGLLRSKGNWSGGGVDKKPSGE
GESPDSSPFVDLGVVSPSSGLGIAFITDNVLLDLELTAMEKGGNGEVISQPKVVTSNKETARILKGTEIPYQEATSEGAT
SVSFKEASLSLEVTPQITPDDQVIMEVRVTKDEPDYLNKLNDVPPIKKNEVNAKVLVRDGETIVLGGVFSKNQSKVVDKV
PFLGDVPYLGRLFRRDVVAQRKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 1332 bp        

>NTDB_id=593244 K1X80_RS03015 WP_220172948.1 681819..683150(+) (pilQ) [Pseudomonas sp. So3.2b]
ATGACTGAGGCTGTGCCCAAGCGAATCGACCTGATTCAATTGCCTCCTCCCGGTAGTTCGCCCTTGAACACAGCGCGTGG
CCCATTTACCGGCAACAGGCTGTCCCTCAACTTTCAGAACATTGAGGTGCGCGCCGTCTTGCAGCAGATTGCCGATGTGG
CCGGGCTCAACCTGGTGGCCGGTGATGATGTGCAAGGCTCGATAACCCTGCGCCTCAAGGACGTGCCGTGGGACCAGGCC
CTGGACCTGGTGTTGCAGGCCAAGGGCCTGGACCGGCGGGTGACCGCCAACGTGCTGCTGGTGGCCCCGGCCGAGGAACT
GGCCGCACGGGAGTTGCTGGCCCTGGAGTCGCACAAGTTGATGACCGAGCTGACGCCACTGCGTCGGGAGCTGCTGCAAG
TCAATTACGCCAAGGCGTCTGAGCTGGCCAAGCTGTTCCAGTCGGTGACCGGGCTGGAGGGGGCGACCGATGAGCGCGGT
TCGGTGACGGTGGATGACCGCACCAACAACATCATCGCCCACCAGACGGGCGAGCGCCTGGAGGAGCTGCGGCGCATCGT
CAGTCAGTTGGATGTGCCGGTACGCCAGGTGATGATCGAGGCGCGGATAGTCGAGGCCAATGTCGACTATGACAAAAGCG
TGGGGGTGCGCTGGGGCGGGTTGCTGCGCAGTAAAGGTAATTGGAGTGGGGGCGGGGTCGATAAAAAACCGTCCGGTGAG
GGCGAGTCGCCCGATAGCTCACCGTTCGTTGACCTGGGCGTGGTCAGCCCGAGCTCCGGCCTGGGCATCGCCTTTATCAC
CGATAACGTGCTGCTGGACCTGGAACTCACGGCCATGGAAAAAGGCGGCAATGGCGAAGTGATCTCCCAGCCCAAGGTGG
TCACCTCCAACAAGGAAACTGCGCGGATTCTCAAGGGCACCGAGATTCCTTACCAGGAAGCCACGTCCGAGGGGGCGACC
TCGGTCTCGTTCAAGGAAGCCTCGCTGTCCCTGGAGGTGACGCCGCAAATCACCCCCGATGACCAGGTGATCATGGAGGT
TCGGGTGACCAAGGATGAGCCCGACTACCTGAACAAACTCAACGATGTGCCGCCGATCAAGAAGAACGAGGTCAACGCCA
AGGTTCTGGTCAGGGACGGCGAGACCATCGTCCTCGGTGGGGTTTTCTCCAAAAACCAAAGCAAGGTGGTAGATAAAGTG
CCATTTTTGGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGTTGCCCAAAGAAAATCCGAGCTGCTGGT
ATTCCTGACTCCGCGTATTATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(57-103)

Bacterial type II/III secretion system short domain (Secretin_N)

(131-196)

Bacterial type II and III secretion system protein (Secretin)

(278-434)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

69.565

98.646

0.686
  pilQ Acinetobacter baumannii D1279779

41.595

100

0.436
  pilQ Acinetobacter baumannii strain A118

41.595

100

0.436
  comQ Acinetobacter baylyi ADP1

42.444

100

0.431
  pilQ Legionella pneumophila strain ERS1305867

44.762

94.808

0.424
  pilQ Legionella pneumophila strain Lp02

44.524

94.808

0.422
  pilQ Vibrio campbellii strain DS40M4

43.735

95.485

0.418
  pilQ Vibrio cholerae strain A1552

43.301

94.357

0.409
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

43.301

94.357

0.409
  pilQ Neisseria gonorrhoeae strain FA1090

36.866

97.968

0.361
  pilQ Neisseria meningitidis MC58

36.446

99.097

0.361
  pilQ Neisseria meningitidis M1080

36.446

99.097

0.361