Detailed information NTG0593045

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: K1T74_RS02465
• Genome accession: NZ_CP080462
• Coordinates: 512248..512646 (-)
• Length: 132 a.a.
• NCBI ID: WP_142732788.1
• Uniprot ID: -
• Organism: Vibrio cholerae strain ICDC-VC702
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	512248..528416	512248..512646	within	0

Gene organization within MGE regions

Location: 512248..528416

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K1T74_RS02465	pilA	512248..512646 (-)	399	WP_142732788.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	Machinery gene
K1T74_RS02470	nadC	512891..513781 (-)	891	WP_000665276.1	carboxylating nicotinate-nucleotide diphosphorylase	-
K1T74_RS02475	ampD	514077..514622 (+)	546	WP_088128396.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
K1T74_RS02480	fldB	514772..515290 (-)	519	WP_000690112.1	flavodoxin FldB	-
K1T74_RS02485	xerD	515428..516336 (+)	909	WP_001287472.1	site-specific tyrosine recombinase XerD	-
K1T74_RS02490	dsbC	516353..517105 (+)	753	WP_000115052.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
K1T74_RS02495	recJ	517164..518903 (+)	1740	WP_220105997.1	single-stranded-DNA-specific exonuclease RecJ	-
K1T74_RS02500	-	518948..520852 (-)	1905	WP_182293216.1	bifunctional UDP-sugar hydrolase/5'-nucleotidase	-
K1T74_RS02505	pdhR	521336..522106 (+)	771	WP_000331318.1	pyruvate dehydrogenase complex transcriptional repressor PdhR	-
K1T74_RS02510	aceE	522156..524816 (+)	2661	WP_001281965.1	pyruvate dehydrogenase (acetyl-transferring), homodimeric type	-
K1T74_RS02515	aceF	524835..526721 (+)	1887	WP_220105998.1	pyruvate dehydrogenase complex dihydrolipoyllysine-residue acetyltransferase	-
K1T74_RS02520	lpdA	526989..528416 (+)	1428	WP_000031534.1	dihydrolipoyl dehydrogenase	-

Sequence

Protein

• Length: 132 a.a.
• Molecular weight: 13701.77 Da
• Isoelectric Point: 9.4205

>NTDB_id=593045 K1T74_RS02465 WP_142732788.1 512248..512646(-) (pilA) [Vibrio cholerae strain ICDC-VC702]
MKAYKNKLQKGFTLIELMIVVAVIGVLAAIAVPQYQKYTQKAALASALATATALKTNYENYFAESGAEPTTSAAIDATPF
SLGTVTVDNTGITVAINKGPAQNSNIKLTRQGLGQWSCTISGAATGITINGC

Nucleotide

• Length: 399 bp

>NTDB_id=593045 K1T74_RS02465 WP_142732788.1 512248..512646(-) (pilA) [Vibrio cholerae strain ICDC-VC702]
ATGAAAGCGTATAAAAACAAACTACAGAAAGGTTTTACCTTAATTGAATTGATGATTGTAGTGGCAGTGATTGGTGTGTT
GGCAGCAATTGCGGTACCGCAGTACCAGAAGTACACACAGAAAGCAGCTTTAGCCAGTGCATTAGCTACAGCAACTGCAT
TAAAAACAAATTATGAAAATTATTTTGCTGAAAGTGGAGCTGAACCAACTACTTCAGCGGCAATTGATGCAACGCCATTT
AGTCTTGGAACAGTAACTGTTGATAACACAGGTATTACAGTAGCAATAAATAAAGGCCCAGCACAAAACTCTAACATTAA
ACTAACAAGGCAAGGATTAGGGCAATGGTCATGTACCATTTCAGGTGCAGCAACTGGCATCACGATTAACGGCTGTTGA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (7-31)

• Pilin (bacterial filament) (Pilin) (41-121)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	47.059	100	0.545
pilA	Vibrio cholerae strain A1552	47.059	100	0.545
pilA	Vibrio cholerae C6706	47.059	100	0.545
pilA	Acinetobacter baumannii strain A118	42.222	100	0.432
pilA	Vibrio parahaemolyticus RIMD 2210633	38.571	100	0.409
pilA/pilA1	Eikenella corrodens VA1	37.589	100	0.402
pilA/pilAII	Pseudomonas stutzeri DSM 10701	39.231	98.485	0.386
pilA/pilAI	Pseudomonas stutzeri DSM 10701	40.8	94.697	0.386
comP	Acinetobacter baylyi ADP1	37.692	98.485	0.371
pilA	Pseudomonas aeruginosa PAK	46.226	80.303	0.371
pilA	Haemophilus influenzae 86-028NP	37.5	96.97	0.364