Detailed information NTG0592404

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: KY495_RS04275
• Genome accession: NZ_CP080378
• Coordinates: 968890..970026 (+)
• Length: 378 a.a.
• NCBI ID: WP_219882515.1
• Uniprot ID: -
• Organism: Massilia sp. PAMC28688
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 963890..975026

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KY495_RS04255 (KY495_04255)	-	964850..965875 (-)	1026	WP_219882512.1	hypothetical protein	-
KY495_RS04260 (KY495_04260)	proC	966208..967026 (-)	819	WP_219882513.1	pyrroline-5-carboxylate reductase	-
KY495_RS04265 (KY495_04265)	-	967041..967742 (-)	702	WP_219884084.1	YggS family pyridoxal phosphate-dependent enzyme	-
KY495_RS04270 (KY495_04270)	pilT	967831..968874 (+)	1044	WP_219882514.1	type IV pilus twitching motility protein PilT	Machinery gene
KY495_RS04275 (KY495_04275)	pilU	968890..970026 (+)	1137	WP_219882515.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
KY495_RS04280 (KY495_04280)	-	970026..970508 (+)	483	WP_219882516.1	glutathione peroxidase	-
KY495_RS04285 (KY495_04285)	-	970671..973646 (-)	2976	WP_219882517.1	SIR2 family protein	-

Sequence

Protein

• Length: 378 a.a.
• Molecular weight: 41879.29 Da
• Isoelectric Point: 6.4187

>NTDB_id=592404 KY495_RS04275 WP_219882515.1 968890..970026(+) (pilU) [Massilia sp. PAMC28688]
MERDQASKFMFDLLRLMTSKGGSDLFITAGFPPAIKIDGKMTPVSSQALTPSHTADLARSIMNDKQTAGFELSKEANFAI
SPGDLGRFRVSAFVQMSCVGMVLRTITTTIPRLDDLGLPEVLKDVVMTKRGLVIMVGATGSGKSTTLAAMVGYRNENSYG
HIITIEDPVEYVHPHRNCVITQREVGVDTETFEAALKNSLRQAPDVIQIGEIRDRETMEHAIAFAETGHLCLATLHANSA
NQALDRIINFFPEERRQQLLMDLSLNLKGLISQRLIPMKETKGRCVAIEIMLNSPLISDLIFKGNVHEIKEIMKKSRELG
MQTFDQALFDLYENDLISYEDALRNADSVNDLRLAIKLKGKDAKNRDLSAGTEHLGIV

Nucleotide

• Length: 1137 bp

>NTDB_id=592404 KY495_RS04275 WP_219882515.1 968890..970026(+) (pilU) [Massilia sp. PAMC28688]
ATGGAACGCGACCAGGCTTCAAAATTCATGTTCGACTTGCTGCGCTTGATGACCAGCAAGGGCGGATCCGACCTGTTCAT
CACCGCCGGCTTCCCGCCCGCAATCAAGATCGACGGCAAGATGACACCGGTGTCGAGCCAGGCGCTGACGCCCTCGCACA
CCGCCGACCTGGCCCGTTCGATCATGAACGACAAGCAGACGGCCGGCTTTGAGCTGTCCAAGGAAGCCAACTTCGCCATC
AGCCCGGGCGACCTGGGACGCTTCCGCGTTTCGGCCTTCGTGCAGATGAGCTGCGTCGGCATGGTACTGCGGACCATCAC
CACCACCATTCCCCGTCTGGACGACCTGGGCCTGCCGGAAGTGCTCAAGGATGTGGTGATGACCAAGCGCGGCCTGGTGA
TCATGGTGGGCGCGACCGGCTCCGGCAAATCGACCACGCTGGCGGCCATGGTTGGCTACCGCAACGAAAACAGCTACGGC
CACATCATCACCATTGAAGACCCGGTGGAATATGTTCACCCGCACCGCAACTGCGTGATCACCCAGCGCGAAGTGGGCGT
GGATACGGAAACGTTTGAAGCGGCGCTGAAAAACTCGCTGCGCCAGGCGCCGGACGTGATCCAGATCGGCGAGATCCGCG
ACCGCGAAACCATGGAGCACGCCATCGCGTTTGCGGAAACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTCGACCGCATCATCAACTTCTTCCCCGAAGAGCGCCGCCAGCAGCTGCTGATGGACCTGTCGCTGAACCT
GAAGGGACTGATCTCGCAGCGCCTGATCCCGATGAAGGAAACCAAGGGTCGCTGCGTGGCGATCGAGATCATGTTAAATT
CTCCCCTGATCTCGGACCTGATCTTCAAGGGCAATGTCCATGAAATCAAGGAGATCATGAAGAAGTCGCGCGAGCTGGGC
ATGCAAACCTTCGACCAGGCGCTGTTCGACCTGTACGAAAATGACCTGATCAGCTACGAAGACGCACTGCGCAACGCCGA
CTCGGTCAACGACCTGCGCCTGGCGATCAAACTCAAGGGCAAGGACGCAAAGAACCGCGACCTGTCGGCAGGCACTGAAC
ACCTGGGGATTGTGTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (99-275)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.708	96.032	0.593
pilU	Acinetobacter baylyi ADP1	58.543	94.444	0.553
pilU	Vibrio cholerae strain A1552	56.936	91.534	0.521
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	44.179	88.624	0.392
pilT	Pseudomonas aeruginosa PAK	43.284	88.624	0.384
pilT	Acinetobacter baylyi ADP1	42.433	89.153	0.378
pilT	Acinetobacter baumannii strain A118	42.9	87.566	0.376
pilT	Acinetobacter baumannii D1279779	42.9	87.566	0.376
pilT	Acinetobacter nosocomialis M2	42.9	87.566	0.376
pilT	Pseudomonas stutzeri DSM 10701	42.09	88.624	0.373