Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ITG12_RS14080 Genome accession   NZ_CP080238
Coordinates   3003994..3004398 (-) Length   134 a.a.
NCBI ID   WP_038878904.1    Uniprot ID   -
Organism   Vibrio sp. ED002     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2998994..3009398
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ITG12_RS14060 (ITG12_13980) coaE 2999498..3000112 (-) 615 WP_038870481.1 dephospho-CoA kinase -
  ITG12_RS14065 (ITG12_13985) pilD 3000113..3000982 (-) 870 WP_038870483.1 A24 family peptidase Machinery gene
  ITG12_RS14070 (ITG12_13990) pilC 3001054..3002277 (-) 1224 WP_038878900.1 type II secretion system F family protein Machinery gene
  ITG12_RS14075 (ITG12_13995) pilB 3002309..3003994 (-) 1686 WP_038878901.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ITG12_RS14080 (ITG12_14000) pilA 3003994..3004398 (-) 405 WP_038878904.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  ITG12_RS14085 (ITG12_14005) nadC 3004663..3005550 (-) 888 WP_038878907.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ITG12_RS14090 (ITG12_14010) ampD 3005643..3006194 (+) 552 WP_038870491.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ITG12_RS14095 (ITG12_14015) pdhR 3006598..3007365 (+) 768 WP_038870493.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 134 a.a.        Molecular weight: 13941.05 Da        Isoelectric Point: 6.2606

>NTDB_id=591588 ITG12_RS14080 WP_038878904.1 3003994..3004398(-) (pilA) [Vibrio sp. ED002]
MKTNKQKKQQGFTLIELMIVVAIIGVLSAVAIPAYKDYVKKSEVASAVATMKSLITPAELYYQENGDITTSTNLGDLGIS
SGSTSLGTLAPKAGQIELSLTNISGAKITITRDANAGWICDATGDAADLVTGCK

Nucleotide


Download         Length: 405 bp        

>NTDB_id=591588 ITG12_RS14080 WP_038878904.1 3003994..3004398(-) (pilA) [Vibrio sp. ED002]
ATGAAAACGAATAAACAGAAGAAGCAGCAAGGTTTTACGCTGATTGAATTGATGATTGTGGTAGCAATTATTGGTGTTTT
GTCAGCTGTAGCTATCCCTGCATATAAAGATTATGTAAAGAAAAGCGAAGTCGCATCTGCCGTTGCAACAATGAAGTCTT
TAATTACACCAGCAGAGCTTTACTACCAAGAAAATGGGGATATCACAACCTCGACTAACTTAGGAGACTTGGGAATATCC
TCTGGCTCAACAAGTTTGGGGACACTTGCACCAAAAGCAGGACAAATTGAGTTGTCATTAACAAACATTAGTGGCGCTAA
AATTACAATAACTAGAGACGCGAATGCTGGTTGGATCTGTGATGCTACAGGTGACGCTGCAGACTTAGTAACTGGCTGTA
AATAG

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(41-133)

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio parahaemolyticus RIMD 2210633

55

100

0.575
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

40.667

100

0.455
  pilA Vibrio cholerae strain A1552

40.667

100

0.455
  pilA Vibrio cholerae C6706

40.667

100

0.455
  pilA Pseudomonas aeruginosa PAK

39.456

100

0.433
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.176

100

0.418
  pilA Haemophilus influenzae 86-028NP

37.879

98.507

0.373
  comP Acinetobacter baylyi ADP1

34.266

100

0.366
  pilA Acinetobacter baumannii strain A118

36.029

100

0.366