Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   HU772_RS23245 Genome accession   NZ_CP077095
Coordinates   5232138..5233370 (-) Length   410 a.a.
NCBI ID   WP_186656737.1    Uniprot ID   A0A9E6Q0L3
Organism   Pseudomonas xantholysinigenes strain RW9S1A     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5227138..5238370
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HU772_RS23230 (HU772_023230) - 5228781..5230403 (-) 1623 WP_186656630.1 AAA family ATPase -
  HU772_RS23235 (HU772_023235) aroB 5230419..5231516 (-) 1098 WP_186656632.1 3-dehydroquinate synthase -
  HU772_RS23240 (HU772_023240) aroK 5231615..5232133 (-) 519 WP_186656639.1 shikimate kinase AroK -
  HU772_RS23245 (HU772_023245) pilQ 5232138..5233370 (-) 1233 WP_186656737.1 type IV pilus secretin PilQ Machinery gene
  HU772_RS23250 (HU772_023250) - 5233330..5234277 (-) 948 WP_186656643.1 pilus assembly protein PilP -
  HU772_RS23255 (HU772_023255) - 5234337..5234876 (-) 540 WP_186656645.1 PilN domain-containing protein -
  HU772_RS23260 (HU772_023260) pilM 5234873..5235721 (-) 849 WP_186656647.1 type IV pilus biogenesis protein PilM -
  HU772_RS23265 (HU772_023265) - 5235891..5238344 (+) 2454 WP_186656649.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 410 a.a.        Molecular weight: 44441.01 Da        Isoelectric Point: 4.8626

>NTDB_id=579198 HU772_RS23245 WP_186656737.1 5232138..5233370(-) (pilQ) [Pseudomonas xantholysinigenes strain RW9S1A]
MLAGMRGALAVEGEPMSLNFQDVEVRAVLQVMADYAGVNLVASDTVQGRITLRLEQVPWQQALDLVLRSRGLGRRQEGNV
LLVAPLAELAERSLGARQEQALEAQLEPLQRALIPVFHAQAAELAELLAASLAEEGGPAGRGSVSVDARTNTLVVALPAQ
RLAELRRLVEQLDLPVRQVMIEARIVEAGVDYEKALGVRWGAPLYGQDSRLGNELFVDLGVERATSGVGVGLLRSDILLD
LELSAMEKSGNGEIISQPKVVTADKETARILKGTEVPYQETTRSGATSISFREASLSLEVTPQITPDGKVVMAVRVTKDE
PDFVNALNNVPPIRKNEVNAKVRVADGETIVIGGVYSTSQNKVVDKVPFFGDLPYVGRLFRRDALQEKKSELLVFLTPRI
MSDQAIAVSR

Nucleotide


Download         Length: 1233 bp        

>NTDB_id=579198 HU772_RS23245 WP_186656737.1 5232138..5233370(-) (pilQ) [Pseudomonas xantholysinigenes strain RW9S1A]
ATGCTGGCGGGGATGCGGGGGGCGCTGGCGGTGGAGGGTGAGCCGATGTCGCTGAATTTCCAGGACGTCGAGGTGCGCGC
GGTGTTGCAGGTCATGGCCGATTATGCCGGTGTCAACCTGGTGGCCAGCGACACCGTGCAGGGGCGCATCACCTTGCGGT
TGGAGCAGGTGCCCTGGCAGCAGGCGCTTGACCTGGTGTTGCGCAGCCGGGGCCTGGGGCGCCGCCAGGAGGGCAACGTG
CTGCTGGTCGCGCCGCTGGCGGAACTGGCCGAGCGATCGCTGGGCGCTCGCCAGGAGCAGGCCCTGGAGGCGCAGTTGGA
GCCTTTGCAGCGCGCGTTGATTCCTGTGTTTCATGCCCAGGCGGCGGAACTCGCCGAGCTGCTGGCGGCCAGTCTTGCCG
AAGAGGGCGGCCCTGCCGGACGTGGCAGTGTGAGCGTGGATGCGCGAACCAATACCTTGGTGGTTGCCCTGCCAGCCCAA
AGGCTGGCGGAACTGCGCAGGCTGGTCGAACAACTGGATCTGCCGGTGCGCCAGGTCATGATCGAGGCGCGGATCGTCGA
GGCCGGCGTCGATTATGAAAAGGCCCTGGGCGTGCGTTGGGGCGCGCCGCTTTACGGCCAGGACAGCCGCCTGGGTAACG
AGCTGTTTGTCGATCTTGGTGTTGAGCGGGCGACGTCGGGTGTCGGCGTGGGGCTATTGCGCAGCGACATTTTGCTCGAC
CTGGAGCTCAGCGCCATGGAAAAAAGCGGCAATGGCGAGATCATTTCCCAGCCCAAGGTGGTCACTGCCGACAAGGAGAC
CGCGCGGATCCTCAAGGGTACCGAGGTGCCGTACCAGGAGACCACCCGCAGTGGCGCCACGTCGATTTCCTTTCGCGAAG
CCTCGCTGTCACTGGAGGTGACCCCGCAGATCACCCCCGACGGCAAGGTGGTGATGGCGGTGCGGGTGACCAAGGACGAG
CCGGATTTTGTCAATGCCCTGAACAATGTGCCGCCGATTCGCAAGAACGAGGTCAATGCCAAGGTGCGTGTGGCCGATGG
CGAAACCATTGTGATTGGTGGCGTGTACTCGACGTCGCAGAACAAAGTGGTCGACAAGGTGCCATTTTTTGGCGATCTGC
CGTATGTTGGACGGCTGTTTCGACGCGATGCATTACAAGAGAAAAAATCCGAGCTGCTGGTCTTCCTGACTCCGCGTATC
ATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(39-85)

Bacterial type II/III secretion system short domain (Secretin_N)

(113-178)

Bacterial type II and III secretion system protein (Secretin)

(245-401)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

58.465

100

0.632
  comQ Acinetobacter baylyi ADP1

42.953

100

0.468
  pilQ Acinetobacter baumannii strain A118

40.7

100

0.454
  pilQ Acinetobacter baumannii D1279779

40.7

100

0.454
  pilQ Legionella pneumophila strain ERS1305867

43.198

100

0.441
  pilQ Legionella pneumophila strain Lp02

43.198

100

0.441
  pilQ Vibrio campbellii strain DS40M4

41.339

100

0.437
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

41.176

100

0.427
  pilQ Vibrio cholerae strain A1552

41.176

100

0.427
  pilQ Neisseria gonorrhoeae strain FA1090

37.019

100

0.376
  pilQ Neisseria meningitidis M1080

36.058

100

0.366
  pilQ Neisseria meningitidis MC58

35.817

100

0.363