Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   HU773_RS02770 Genome accession   NZ_CP077085
Coordinates   618062..619393 (+) Length   443 a.a.
NCBI ID   WP_057958081.1    Uniprot ID   -
Organism   Pseudomonas shahriarae strain SWRI52     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 613062..624393
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HU773_RS02750 (HU773_002750) pilM 615659..616312 (+) 654 WP_057437230.1 type IV pilus biogenesis protein PilM -
  HU773_RS02755 (HU773_002755) - 616312..616863 (+) 552 WP_057437231.1 PilN domain-containing protein -
  HU773_RS02760 (HU773_002760) - 616860..617480 (+) 621 WP_186625789.1 type 4a pilus biogenesis protein PilO -
  HU773_RS02765 (HU773_002765) - 617477..617992 (+) 516 WP_186625790.1 pilus assembly protein PilP -
  HU773_RS02770 (HU773_002770) pilQ 618062..619393 (+) 1332 WP_057958081.1 type IV pilus secretin PilQ Machinery gene
  HU773_RS02775 (HU773_002775) aroK 619398..619916 (+) 519 WP_057437234.1 shikimate kinase AroK -
  HU773_RS02780 (HU773_002780) aroB 620064..621164 (+) 1101 WP_057958082.1 3-dehydroquinate synthase -
  HU773_RS02785 (HU773_002785) - 621175..622749 (+) 1575 WP_120731330.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 443 a.a.        Molecular weight: 48279.16 Da        Isoelectric Point: 4.8378

>NTDB_id=578931 HU773_RS02770 WP_057958081.1 618062..619393(+) (pilQ) [Pseudomonas shahriarae strain SWRI52]
MTEAVPKRIDLIQLPPPGSSPLNTARGPFTGNRLSLNFQDIEVRAVLQQIADVAGLNLVAGDDVQGSITLRLKDVPWDQA
LDLVLQAKGLDRRVTANVLLVAPAEELAARELLALESRKLMTELTPLRRELLQVNYAKASELAKLFQSVTGLEGATDERG
SVTVDDRTNNIIAYQTGERLEELRRIVSQLDVPVRQVMIEARIVEANVDYDKSVGVRWGGLLRSKGNWSGGGVDKKPSGE
GESPDSSPFVDLGVVSPSSGLGIAFITDNVLLDLELTAMEKGGNGEVISQPKVVTSNKETARILKGTEIPYQEATSEGAT
SVSFKEASLSLEVTPQITPDDQVIMEVRVTKDEPDYLNKLNDVPPIKKNEVNAKVLVRDGETIVLGGVFSKNQSKVVDKV
PFLGDVPYLGRLFRRDVVAQRKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 1332 bp        

>NTDB_id=578931 HU773_RS02770 WP_057958081.1 618062..619393(+) (pilQ) [Pseudomonas shahriarae strain SWRI52]
ATGACTGAGGCTGTGCCCAAGCGAATCGACCTGATTCAATTGCCTCCTCCCGGTAGTTCGCCCTTGAACACAGCGCGTGG
CCCATTTACCGGCAACAGGCTGTCCCTCAACTTTCAGGACATTGAGGTGCGCGCCGTCTTGCAGCAGATTGCCGATGTGG
CCGGGCTCAACCTGGTGGCCGGTGATGATGTGCAAGGCTCGATAACCCTGCGCCTCAAGGATGTGCCGTGGGACCAGGCC
CTGGACCTGGTGTTGCAGGCCAAGGGCCTGGACCGGCGGGTGACCGCCAACGTGCTGCTGGTGGCCCCGGCCGAGGAACT
GGCCGCACGGGAGTTGCTGGCCCTGGAGTCGCGCAAGTTGATGACCGAGCTGACGCCACTGCGTCGGGAGCTGCTGCAAG
TCAATTACGCCAAGGCGTCTGAGCTGGCCAAGCTGTTCCAGTCGGTGACCGGGCTGGAGGGGGCGACCGATGAGCGCGGT
TCGGTGACGGTGGATGACCGCACCAACAACATCATCGCCTACCAGACGGGCGAGCGCCTGGAGGAGCTGCGGCGCATCGT
CAGTCAGTTGGATGTGCCGGTACGCCAGGTGATGATCGAGGCGCGGATAGTCGAGGCCAATGTCGACTATGACAAAAGCG
TGGGGGTGCGCTGGGGCGGGTTGCTGCGCAGTAAAGGTAATTGGAGTGGGGGCGGGGTCGATAAAAAACCGTCCGGTGAG
GGCGAGTCGCCCGATAGCTCACCGTTCGTTGACCTGGGCGTGGTCAGCCCGAGCTCCGGCCTGGGCATCGCCTTTATCAC
CGATAACGTGCTGCTGGACCTGGAACTCACGGCCATGGAAAAAGGCGGCAATGGCGAAGTGATCTCCCAGCCCAAGGTGG
TCACCTCCAACAAGGAAACTGCGCGGATTCTCAAGGGCACCGAGATTCCTTACCAGGAAGCCACGTCCGAGGGGGCGACC
TCGGTCTCGTTCAAGGAAGCCTCGCTGTCCCTGGAGGTGACGCCGCAAATCACCCCCGATGACCAGGTGATCATGGAGGT
TCGGGTGACCAAGGATGAGCCCGACTACCTGAACAAACTCAACGATGTGCCGCCGATCAAGAAGAACGAGGTCAACGCCA
AGGTTCTGGTCAGGGACGGCGAGACCATCGTCCTCGGTGGGGTTTTCTCCAAAAACCAAAGCAAGGTGGTAGATAAAGTG
CCATTTTTGGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGTTGCCCAAAGAAAATCCGAGCTGCTGGT
ATTCCTGACTCCGCGTATTATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(278-434)

Secretin and TonB N terminus short domain (STN)

(57-103)

Bacterial type II/III secretion system short domain (Secretin_N)

(131-196)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

70.023

98.646

0.691
  pilQ Acinetobacter baumannii D1279779

41.81

100

0.438
  pilQ Acinetobacter baumannii strain A118

41.81

100

0.438
  comQ Acinetobacter baylyi ADP1

42.667

100

0.433
  pilQ Legionella pneumophila strain ERS1305867

44.524

94.808

0.422
  pilQ Vibrio campbellii strain DS40M4

43.972

95.485

0.42
  pilQ Legionella pneumophila strain Lp02

44.286

94.808

0.42
  pilQ Vibrio cholerae strain A1552

43.541

94.357

0.411
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

43.541

94.357

0.411
  pilQ Neisseria gonorrhoeae strain FA1090

37.097

97.968

0.363
  pilQ Neisseria meningitidis MC58

36.674

99.097

0.363
  pilQ Neisseria meningitidis M1080

36.674

99.097

0.363