Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   KSS90_RS23780 Genome accession   NZ_CP077077
Coordinates   5350155..5351384 (-) Length   409 a.a.
NCBI ID   WP_225933107.1    Uniprot ID   -
Organism   Pseudomonas maumuensis strain COW77     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5345155..5356384
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KSS90_RS23765 (KSS90_23765) - 5346801..5348423 (-) 1623 WP_217867507.1 AAA family ATPase -
  KSS90_RS23770 (KSS90_23770) aroB 5348439..5349536 (-) 1098 WP_217867508.1 3-dehydroquinate synthase -
  KSS90_RS23775 (KSS90_23775) aroK 5349632..5350150 (-) 519 WP_038706967.1 shikimate kinase AroK -
  KSS90_RS23780 (KSS90_23780) pilQ 5350155..5351384 (-) 1230 WP_225933107.1 type IV pilus secretin PilQ Machinery gene
  KSS90_RS23785 (KSS90_23785) - 5351335..5352345 (-) 1011 WP_217867510.1 pilus assembly protein PilP -
  KSS90_RS23790 (KSS90_23790) - 5352342..5352881 (-) 540 WP_217867511.1 PilN domain-containing protein -
  KSS90_RS23795 (KSS90_23795) pilM 5352878..5353771 (-) 894 WP_217867512.1 type IV pilus biogenesis protein PilM -

Sequence

Protein


Download         Length: 409 a.a.        Molecular weight: 44374.10 Da        Isoelectric Point: 4.8050

>NTDB_id=578740 KSS90_RS23780 WP_225933107.1 5350155..5351384(-) (pilQ) [Pseudomonas maumuensis strain COW77]
MGLVSILGVQAAEFQGEPMSLDFQDVELRSVLQVMAEHAGVNLLASDSVQGRITLRLEEVPWDQALDLVLRSKGLGKRQE
GNVLLIAPLAELADQARRMAQDVSMEPLRRELIPVFHAKAGELVELLLASLADDGILAGRGSLGVDARTNTLVVTQPAGR
LVELRRLVAELDVPVRQVMIEARIVEASVDYEKALGVRWGGPLYGAESRLGSDLFVDLGVERAGAGLGLGLLRSDILLDL
ELTAMEKSGNGEIISQPKVVTADKETARILKGTEVPYQETTRSGATSVSFREASLSLEVTPQITPDGKVIMAVRVTKDEP
DFVNALNDVPPIRKNEVNAKVRVADGQTIVIGGVYSTSQNKVVDKVPFFGDLPYVGRLFRRDALQEKKSELLVFLTPRIM
SDQAIAVSR

Nucleotide


Download         Length: 1230 bp        

>NTDB_id=578740 KSS90_RS23780 WP_225933107.1 5350155..5351384(-) (pilQ) [Pseudomonas maumuensis strain COW77]
ATGGGTCTGGTGAGCATCCTGGGGGTGCAGGCAGCGGAGTTTCAGGGTGAGCCGATGTCGCTCGACTTCCAGGATGTCGA
GCTGCGCTCGGTGCTTCAGGTCATGGCGGAGCATGCCGGCGTCAACCTGCTGGCCAGCGATTCGGTGCAGGGGCGAATAA
CTCTGCGCCTGGAAGAAGTGCCTTGGGACCAGGCGCTCGATTTGGTGCTGCGCAGCAAGGGATTGGGCAAGCGGCAGGAG
GGAAATGTGCTGCTGATTGCCCCGCTGGCCGAACTCGCCGACCAGGCGCGTAGAATGGCGCAGGATGTGTCGATGGAGCC
ATTGCGGCGCGAGCTGATCCCGGTCTTCCATGCCAAGGCCGGCGAGTTGGTGGAGTTACTGCTGGCCAGCCTGGCCGATG
ATGGCATTCTGGCTGGGCGTGGCAGCCTGGGTGTAGATGCCCGCACCAACACGCTGGTGGTCACACAGCCCGCCGGACGG
CTAGTCGAACTACGCAGGCTGGTGGCGGAACTGGATGTGCCGGTGCGCCAGGTCATGATCGAGGCGCGCATCGTCGAGGC
CAGCGTCGATTACGAGAAGGCGCTCGGTGTGCGTTGGGGTGGTCCGTTGTATGGTGCGGAGAGTCGGTTGGGTAGTGATT
TGTTCGTCGACCTCGGCGTCGAGCGGGCTGGCGCCGGGCTTGGGTTGGGGCTGCTGCGCAGCGACATCCTCCTGGACCTG
GAGCTCACTGCCATGGAAAAAAGCGGCAACGGCGAGATCATCTCCCAGCCAAAAGTGGTAACCGCCGACAAGGAAACAGC
CCGTATCCTCAAGGGCACCGAAGTGCCCTATCAGGAAACCACCCGCAGTGGCGCCACTTCCGTGTCCTTTCGCGAGGCCT
CCCTGTCGCTTGAGGTGACGCCGCAGATCACGCCGGACGGCAAGGTGATCATGGCGGTGCGGGTGACCAAGGACGAGCCC
GACTTCGTCAATGCTTTGAACGATGTGCCGCCGATTCGCAAGAACGAGGTCAACGCCAAGGTTCGGGTGGCCGACGGGCA
GACCATTGTGATTGGCGGGGTATATTCGACGTCGCAGAACAAAGTGGTCGACAAGGTGCCATTTTTCGGCGATCTGCCGT
ATGTTGGGCGGCTATTTCGACGCGATGCACTACAAGAGAAAAAATCCGAGCTGCTGGTCTTCCTGACTCCGCGTATCATG
AGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(244-400)

Secretin and TonB N terminus short domain (STN)

(41-88)

Bacterial type II/III secretion system short domain (Secretin_N)

(112-177)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

56.881

100

0.606
  pilQ Acinetobacter baumannii D1279779

41.775

100

0.472
  pilQ Acinetobacter baumannii strain A118

41.775

100

0.472
  comQ Acinetobacter baylyi ADP1

43.049

100

0.469
  pilQ Legionella pneumophila strain ERS1305867

43.541

100

0.445
  pilQ Legionella pneumophila strain Lp02

43.062

100

0.44
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

39.286

100

0.403
  pilQ Vibrio cholerae strain A1552

39.286

100

0.403
  pilQ Vibrio campbellii strain DS40M4

38.443

100

0.399
  pilQ Neisseria gonorrhoeae strain FA1090

37.56

100

0.384
  pilQ Neisseria meningitidis M1080

36.842

100

0.377
  pilQ Neisseria meningitidis MC58

36.364

100

0.372