Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   KM433_RS05735 Genome accession   NZ_CP076257
Coordinates   1282733..1283143 (+) Length   136 a.a.
NCBI ID   WP_039955568.1    Uniprot ID   A0A1M4L1M0
Organism   Xanthomonas translucens pv. graminis strain ART-Xtg29     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1284111..1297848 1282733..1283143 flank 968


Gene organization within MGE regions


Location: 1282733..1297848
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KM433_RS05735 (KM433_05720) pilA/pilAI 1282733..1283143 (+) 411 WP_039955568.1 pilin Machinery gene
  KM433_RS05740 - 1283805..1284110 (-) 306 WP_231103532.1 hypothetical protein -
  KM433_RS05745 - 1284111..1284362 (-) 252 WP_009580414.1 transposase -
  KM433_RS05750 (KM433_05730) - 1284462..1284782 (-) 321 WP_009580416.1 DUF86 domain-containing protein -
  KM433_RS05755 (KM433_05735) - 1284779..1285069 (-) 291 WP_009580418.1 nucleotidyltransferase family protein -
  KM433_RS05760 (KM433_05740) pilB 1285276..1287012 (+) 1737 WP_009580420.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KM433_RS05765 (KM433_05745) - 1287040..1287333 (-) 294 WP_009580422.1 addiction module antidote protein -
  KM433_RS05770 (KM433_05750) - 1287336..1287632 (-) 297 WP_009580424.1 type II toxin-antitoxin system RelE/ParE family toxin -
  KM433_RS05775 (KM433_05755) pilR 1287797..1289185 (-) 1389 WP_009580426.1 sigma-54-dependent transcriptional regulator Regulator
  KM433_RS05780 (KM433_05760) - 1289260..1290873 (-) 1614 WP_009580428.1 sensor histidine kinase -
  KM433_RS05785 (KM433_05765) sucC 1291172..1292335 (+) 1164 WP_009580518.1 ADP-forming succinate--CoA ligase subunit beta -
  KM433_RS05790 (KM433_05770) sucD 1292359..1293231 (+) 873 WP_009580520.1 succinate--CoA ligase subunit alpha -
  KM433_RS05795 (KM433_05775) - 1293631..1295724 (+) 2094 WP_009580522.1 pyrroloquinoline quinone-dependent dehydrogenase -
  KM433_RS05800 (KM433_05780) - 1295965..1297848 (-) 1884 WP_009580524.1 hypothetical protein -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14145.58 Da        Isoelectric Point: 8.4919

>NTDB_id=574110 KM433_RS05735 WP_039955568.1 1282733..1283143(+) (pilA/pilAI) [Xanthomonas translucens pv. graminis strain ART-Xtg29]
MKKQQGFTLIELMIVIAIIAILAAIALPMYQDYVAKSQVTAGLAEINPGKAQYEVALNEGKTAITGIGDLGLKSPTDRCT
ITDITALSPTGSIVCTLKGNTQVLGKTVTLKRNTDGSWTCATTVLAKHAPAGCPGV

Nucleotide


Download         Length: 411 bp        

>NTDB_id=574110 KM433_RS05735 WP_039955568.1 1282733..1283143(+) (pilA/pilAI) [Xanthomonas translucens pv. graminis strain ART-Xtg29]
ATGAAGAAGCAACAGGGCTTTACGCTGATCGAACTGATGATCGTCATTGCGATCATCGCCATTTTGGCCGCCATTGCGCT
GCCGATGTACCAGGATTATGTCGCCAAGTCGCAAGTAACCGCTGGTCTTGCTGAGATCAACCCGGGTAAGGCCCAGTACG
AAGTTGCATTGAACGAAGGTAAGACCGCTATTACCGGCATTGGCGATCTTGGTTTGAAGTCTCCTACTGACCGTTGCACT
ATCACGGACATTACTGCGCTGAGTCCCACGGGAAGCATCGTTTGTACTTTGAAGGGTAACACCCAGGTTCTCGGCAAGAC
GGTTACCCTGAAACGTAATACTGATGGTTCCTGGACTTGTGCTACCACTGTTTTGGCGAAGCACGCTCCGGCCGGTTGCC
CGGGTGTTTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-133)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1M4L1M0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

52.593

99.265

0.522
  pilA Acinetobacter baumannii strain A118

51.095

100

0.515
  pilA Pseudomonas aeruginosa PAK

40.94

100

0.449
  pilA/pilAII Pseudomonas stutzeri DSM 10701

45.522

98.529

0.449
  pilA2 Legionella pneumophila str. Paris

43.796

100

0.441
  pilA2 Legionella pneumophila strain ERS1305867

43.066

100

0.434
  pilA Vibrio cholerae C6706

41.429

100

0.426
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

41.429

100

0.426
  pilA Vibrio cholerae strain A1552

41.429

100

0.426
  pilE Neisseria gonorrhoeae MS11

40.741

99.265

0.404
  comP Acinetobacter baylyi ADP1

35.57

100

0.39