Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   KHO27_RS20355 Genome accession   NZ_CP075595
Coordinates   4470957..4472180 (-) Length   407 a.a.
NCBI ID   WP_214502011.1    Uniprot ID   -
Organism   Pseudomonas sp. RC3H12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4465957..4477180
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KHO27_RS20340 (KHO27_20340) - 4467600..4469225 (-) 1626 WP_214500693.1 AAA family ATPase -
  KHO27_RS20345 (KHO27_20345) aroB 4469241..4470338 (-) 1098 WP_023631794.1 3-dehydroquinate synthase -
  KHO27_RS20350 (KHO27_20350) aroK 4470434..4470952 (-) 519 WP_038706967.1 shikimate kinase AroK -
  KHO27_RS20355 (KHO27_20355) pilQ 4470957..4472180 (-) 1224 WP_214502011.1 type IV pilus secretin PilQ Machinery gene
  KHO27_RS20360 (KHO27_20360) - 4472137..4473150 (-) 1014 WP_110697375.1 pilus assembly protein PilP -
  KHO27_RS20365 (KHO27_20365) - 4473147..4473686 (-) 540 WP_146230396.1 PilN domain-containing protein -
  KHO27_RS20370 (KHO27_20370) pilM 4473683..4474579 (-) 897 WP_214500694.1 pilus assembly protein PilM -

Sequence

Protein


Download         Length: 407 a.a.        Molecular weight: 44274.90 Da        Isoelectric Point: 4.7487

>NTDB_id=569807 KHO27_RS20355 WP_214502011.1 4470957..4472180(-) (pilQ) [Pseudomonas sp. RC3H12]
MLSILAVQAAELQGEPMSLNFQDVELRSVLKVMAEHAGVNLVASDSVQGRITLRLEDVPWEQALDLVLRSKGLGRRQEGN
VLLIAPVAELAGQARGMSEEVLLEPLRRELIPVFHAKAAELAELLLASLADDGILTGRGSLSVDARTNTLVVTQPGERLA
ELRRLVTQLDVPVRQVMIEARIVEASVDYEKVLGVRWGGPLYGGDSSLGSDLFVDLGVERANAGLGVGLLRSDILLDLEL
SAMEKSGNGEIISQPKVVTADKETARILKGTEVPYQETTRSGATSISFREASLSLEVTPQITPDGKVVMDVRVTKDEPDF
INALNDVPPIRRNEVTAKVRVTDGQTIVIGGVYSTSQNKVVDKVPFFGDLPYVGRLFRRDVLQEKKSELLVFLTPRIMSD
QAIAVSR

Nucleotide


Download         Length: 1224 bp        

>NTDB_id=569807 KHO27_RS20355 WP_214502011.1 4470957..4472180(-) (pilQ) [Pseudomonas sp. RC3H12]
ATGTTGAGCATCCTGGCGGTGCAGGCAGCGGAGCTTCAGGGTGAGCCGATGTCGCTCAACTTCCAGGATGTCGAGCTGCG
CTCGGTGCTTAAGGTCATGGCCGAGCACGCCGGCGTCAACCTGGTTGCCAGTGATTCGGTGCAGGGACGGATAACCCTGC
GCCTGGAGGACGTGCCCTGGGAGCAGGCGCTCGACCTGGTGCTGCGCAGCAAGGGGCTGGGTAGGCGCCAGGAGGGCAAT
GTGCTGCTGATCGCCCCCGTTGCGGAACTGGCCGGGCAGGCCCGGGGTATGAGCGAGGAGGTGTTGCTGGAGCCCTTGCG
GCGTGAACTGATCCCGGTCTTTCATGCCAAGGCCGCCGAGTTGGCGGAGCTGTTGCTGGCGAGCCTGGCCGATGACGGCA
TTCTGACGGGGCGCGGCAGCCTCAGTGTGGATGCGCGGACCAACACGCTGGTGGTCACCCAGCCTGGCGAACGGCTTGCC
GAGCTGCGCAGGCTGGTGACGCAACTGGATGTGCCGGTGCGCCAGGTCATGATCGAGGCGCGTATCGTCGAGGCCAGCGT
CGATTACGAGAAGGTGCTGGGCGTGCGATGGGGTGGCCCGTTGTATGGGGGGGACAGTAGCCTGGGTAGCGACCTGTTCG
TCGATCTCGGGGTCGAGCGGGCCAATGCCGGGCTCGGCGTGGGGTTGCTGCGCAGCGACATTCTGCTGGACTTGGAGCTC
AGCGCCATGGAGAAGAGCGGCAATGGCGAGATCATCTCCCAGCCCAAGGTGGTCACGGCCGACAAGGAGACTGCGCGCAT
ACTCAAGGGCACAGAAGTGCCGTACCAGGAGACCACGCGCAGTGGTGCCACCTCGATTTCCTTTCGCGAGGCCTCCCTGT
CGCTGGAGGTGACGCCGCAGATCACCCCGGATGGCAAGGTAGTGATGGATGTGCGTGTGACCAAGGATGAGCCCGACTTC
ATCAATGCGCTGAACGATGTGCCGCCGATCCGCAGGAACGAGGTTACGGCGAAGGTCCGGGTGACCGATGGGCAGACCAT
TGTGATTGGCGGCGTGTACTCGACGTCGCAGAACAAAGTGGTCGACAAGGTGCCATTTTTCGGCGATCTGCCGTATGTTG
GGCGGCTGTTTCGACGCGATGTATTACAAGAGAAAAAATCCGAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGAC
CAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(110-175)

Secretin and TonB N terminus short domain (STN)

(40-86)

Bacterial type II and III secretion system protein (Secretin)

(242-398)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

57.604

100

0.614
  pilQ Acinetobacter baumannii D1279779

41.667

100

0.467
  pilQ Acinetobacter baumannii strain A118

41.667

100

0.467
  comQ Acinetobacter baylyi ADP1

42.664

100

0.464
  pilQ Legionella pneumophila strain Lp02

44.286

100

0.457
  pilQ Legionella pneumophila strain ERS1305867

44.286

100

0.457
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

41.667

100

0.43
  pilQ Vibrio cholerae strain A1552

41.667

100

0.43
  pilQ Vibrio campbellii strain DS40M4

41.038

100

0.428
  pilQ Neisseria gonorrhoeae strain FA1090

36.429

100

0.376
  pilQ Neisseria meningitidis MC58

35.646

100

0.366
  pilQ Neisseria meningitidis M1080

35.646

100

0.366