Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   KCG54_RS08880 Genome accession   NZ_CP073115
Coordinates   1841629..1842102 (+) Length   157 a.a.
NCBI ID   WP_254323969.1    Uniprot ID   -
Organism   Neisseria subflava strain TT0077     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1836629..1847102
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KCG54_RS08865 (KCG54_08900) - 1837096..1837536 (+) 441 WP_254323967.1 TIGR01244 family sulfur transferase -
  KCG54_RS08870 (KCG54_08905) - 1837963..1838259 (+) 297 WP_003680870.1 helix-turn-helix transcriptional regulator -
  KCG54_RS08875 (KCG54_08910) glnD 1838279..1840837 (+) 2559 WP_254323968.1 [protein-PII] uridylyltransferase -
  KCG54_RS08880 (KCG54_08915) pilE 1841629..1842102 (+) 474 WP_254323969.1 pilin Machinery gene
  KCG54_RS08885 (KCG54_08920) - 1842217..1842675 (+) 459 WP_254323970.1 pilin -
  KCG54_RS08890 (KCG54_08925) - 1843101..1844627 (-) 1527 WP_254323971.1 DHA2 family efflux MFS transporter permease subunit -
  KCG54_RS08895 (KCG54_08930) - 1844729..1845919 (-) 1191 WP_070823422.1 efflux RND transporter periplasmic adaptor subunit -

Sequence

Protein


Download         Length: 157 a.a.        Molecular weight: 16697.14 Da        Isoelectric Point: 8.4489

>NTDB_id=558046 KCG54_RS08880 WP_254323969.1 1841629..1842102(+) (pilE) [Neisseria subflava strain TT0077]
MKAIQKGFTLIELMIVIAIIGILAVIALPAYQDYTARAQVSEAISLMEGQKSAVVEYYADKGKWPTSNEEAGIATNTSIQ
GKYVAQVDVGANGVITAKMKGENVNNEIKNKTVSLTPHATTNNGSFTWTCAPGKAEGTTGVDTKFLPSSCRPAVEKK

Nucleotide


Download         Length: 474 bp        

>NTDB_id=558046 KCG54_RS08880 WP_254323969.1 1841629..1842102(+) (pilE) [Neisseria subflava strain TT0077]
ATGAAAGCAATCCAAAAAGGTTTCACCCTGATCGAATTGATGATCGTCATCGCGATTATCGGCATCTTGGCAGTTATCGC
ACTGCCTGCATACCAAGACTACACTGCACGTGCCCAAGTTTCTGAAGCCATCTCTTTGATGGAAGGTCAAAAGTCTGCTG
TGGTTGAATACTATGCAGATAAAGGTAAATGGCCTACTAGCAATGAAGAAGCAGGTATCGCAACAAATACAAGCATTCAA
GGTAAATATGTTGCACAAGTAGATGTCGGAGCTAATGGTGTTATTACTGCCAAAATGAAAGGCGAAAATGTAAATAACGA
AATTAAAAATAAAACCGTATCTCTTACTCCTCATGCTACCACTAACAATGGCTCCTTTACTTGGACTTGTGCCCCAGGTA
AGGCCGAAGGCACCACAGGCGTAGATACAAAATTCTTACCTTCTTCTTGCCGCCCAGCTGTGGAAAAAAAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(4-28)

Pilin (bacterial filament) (Pilin)

(36-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae MS11

58.025

100

0.599
  pilE Neisseria gonorrhoeae strain FA1090

55.901

100

0.573
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

37.895

100

0.459
  pilA/pilA1 Eikenella corrodens VA1

44.099

100

0.452
  comP Acinetobacter baylyi ADP1

39.286

100

0.42
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.935

98.726

0.414
  pilA2 Legionella pneumophila str. Paris

43.243

94.268

0.408
  pilA2 Legionella pneumophila strain ERS1305867

42.568

94.268

0.401
  pilA Pseudomonas aeruginosa PAK

36.81

100

0.382
  pilA Acinetobacter baumannii strain A118

40.268

94.904

0.382
  pilA Ralstonia pseudosolanacearum GMI1000

35.329

100

0.376