Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   J9537_RS00885 Genome accession   NZ_CP072888
Coordinates   165043..165480 (+) Length   145 a.a.
NCBI ID   WP_218256382.1    Uniprot ID   -
Organism   Enterococcus raffinosus strain F162_2     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 151906..197463 165043..165480 within 0


Gene organization within MGE regions


Location: 151906..197463
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J9537_RS00770 (J9537_00770) recA 151906..152955 (+) 1050 WP_218256362.1 recombinase RecA Machinery gene
  J9537_RS00775 (J9537_00775) rny 153199..154755 (+) 1557 WP_010747259.1 ribonuclease Y -
  J9537_RS00780 (J9537_00780) - 154835..155977 (-) 1143 WP_218256363.1 site-specific integrase -
  J9537_RS00785 (J9537_00785) - 155990..156301 (-) 312 WP_218256364.1 hypothetical protein -
  J9537_RS00790 (J9537_00790) - 156303..156473 (-) 171 WP_218256365.1 hypothetical protein -
  J9537_RS00795 (J9537_00795) - 156580..157221 (-) 642 WP_218256366.1 hypothetical protein -
  J9537_RS00800 (J9537_00800) - 157296..158000 (-) 705 WP_218256367.1 XRE family transcriptional regulator -
  J9537_RS00805 (J9537_00805) - 158191..158403 (+) 213 WP_218256368.1 helix-turn-helix transcriptional regulator -
  J9537_RS00810 (J9537_00810) - 158452..159213 (+) 762 WP_218256369.1 phage antirepressor KilAC domain-containing protein -
  J9537_RS00815 (J9537_00815) - 159225..159530 (+) 306 WP_218256370.1 hypothetical protein -
  J9537_RS00820 (J9537_00820) - 159544..159720 (+) 177 WP_218256371.1 hypothetical protein -
  J9537_RS00825 (J9537_00825) - 159725..159907 (+) 183 WP_218256372.1 hypothetical protein -
  J9537_RS00830 (J9537_00830) - 159900..160139 (-) 240 WP_218256373.1 hypothetical protein -
  J9537_RS00835 (J9537_00835) - 160191..160343 (+) 153 WP_176673295.1 hypothetical protein -
  J9537_RS00840 (J9537_00840) - 160420..160758 (-) 339 WP_218256374.1 hypothetical protein -
  J9537_RS00845 (J9537_00845) - 160815..160961 (+) 147 WP_218256375.1 hypothetical protein -
  J9537_RS00850 (J9537_00850) - 160997..161224 (+) 228 WP_218256376.1 hypothetical protein -
  J9537_RS00855 (J9537_00855) - 161237..161773 (+) 537 WP_218256377.1 host-nuclease inhibitor Gam family protein -
  J9537_RS00860 (J9537_00860) - 161775..162773 (+) 999 WP_144319158.1 AAA family ATPase -
  J9537_RS00865 (J9537_00865) - 162778..163542 (+) 765 WP_218256378.1 putative HNHc nuclease -
  J9537_RS00870 (J9537_00870) - 163532..164332 (+) 801 WP_218256379.1 helix-turn-helix domain-containing protein -
  J9537_RS00875 (J9537_00875) - 164325..164633 (+) 309 WP_218256380.1 hypothetical protein -
  J9537_RS00880 (J9537_00880) - 164630..165046 (+) 417 WP_218256381.1 RusA family crossover junction endodeoxyribonuclease -
  J9537_RS00885 (J9537_00885) ssb 165043..165480 (+) 438 WP_218256382.1 single-stranded DNA-binding protein Machinery gene
  J9537_RS00890 (J9537_00890) - 165492..165713 (+) 222 WP_218256383.1 hypothetical protein -
  J9537_RS00895 (J9537_00895) - 165715..165981 (+) 267 WP_185969649.1 hypothetical protein -
  J9537_RS00900 (J9537_00900) - 165983..166237 (+) 255 WP_218256384.1 hypothetical protein -
  J9537_RS00905 (J9537_00905) - 166212..167213 (+) 1002 WP_218257301.1 tyrosine-type recombinase/integrase -
  J9537_RS00910 (J9537_00910) - 167282..167548 (+) 267 WP_218256385.1 hypothetical protein -
  J9537_RS00915 (J9537_00915) - 167615..167869 (+) 255 WP_218256386.1 hypothetical protein -
  J9537_RS00920 (J9537_00920) - 167927..168664 (+) 738 WP_218256387.1 hypothetical protein -
  J9537_RS00925 (J9537_00925) - 168689..169090 (+) 402 WP_218256388.1 hypothetical protein -
  J9537_RS00930 (J9537_00930) - 169124..169543 (+) 420 WP_218256389.1 hypothetical protein -
  J9537_RS00935 (J9537_00935) - 169574..170524 (+) 951 WP_218256390.1 site-specific integrase -
  J9537_RS00940 (J9537_00940) - 170538..171056 (+) 519 WP_218256391.1 hypothetical protein -
  J9537_RS00945 (J9537_00945) - 171059..171652 (+) 594 WP_218256392.1 N-6 DNA methylase -
  J9537_RS00950 (J9537_00950) - 171720..171986 (+) 267 WP_218256393.1 hypothetical protein -
  J9537_RS00955 (J9537_00955) - 172009..172458 (+) 450 WP_218256394.1 hypothetical protein -
  J9537_RS00960 (J9537_00960) - 173363..173587 (+) 225 WP_218256395.1 hypothetical protein -
  J9537_RS00965 (J9537_00965) - 173613..173969 (+) 357 WP_218256396.1 phBC6A51 family helix-turn-helix protein -
  J9537_RS00970 (J9537_00970) - 173962..175245 (+) 1284 WP_245001305.1 PBSX family phage terminase large subunit -
  J9537_RS00975 (J9537_00975) - 175258..176679 (+) 1422 WP_218256397.1 phage portal protein -
  J9537_RS00980 (J9537_00980) - 176672..176875 (+) 204 WP_218256398.1 hypothetical protein -
  J9537_RS00985 (J9537_00985) - 176880..177188 (+) 309 WP_218256399.1 ribosomal-processing cysteine protease Prp -
  J9537_RS00990 (J9537_00990) - 177281..177502 (-) 222 WP_218256400.1 hypothetical protein -
  J9537_RS00995 (J9537_00995) - 177580..178968 (+) 1389 WP_218256401.1 minor capsid protein -
  J9537_RS01000 (J9537_01000) - 179032..179214 (+) 183 WP_245001270.1 hypothetical protein -
  J9537_RS01005 (J9537_01005) - 179333..179971 (+) 639 WP_218256403.1 DUF4355 domain-containing protein -
  J9537_RS01010 (J9537_01010) - 179987..181027 (+) 1041 WP_218256404.1 major capsid protein -
  J9537_RS01015 (J9537_01015) - 181040..181387 (+) 348 WP_218256405.1 phage head-tail connector protein -
  J9537_RS01020 (J9537_01020) - 181368..181721 (+) 354 WP_218256406.1 hypothetical protein -
  J9537_RS01025 (J9537_01025) - 181711..182250 (+) 540 WP_218256407.1 HK97 gp10 family phage protein -
  J9537_RS01030 (J9537_01030) - 182247..182612 (+) 366 WP_010743585.1 hypothetical protein -
  J9537_RS01035 (J9537_01035) - 182628..183065 (+) 438 WP_218256408.1 phage tail protein -
  J9537_RS01040 (J9537_01040) - 183136..183507 (+) 372 WP_218256409.1 DUF6096 family protein -
  J9537_RS01045 (J9537_01045) - 183540..183887 (+) 348 WP_185937995.1 hypothetical protein -
  J9537_RS20570 - 183891..185417 (+) 1527 WP_245001271.1 hypothetical protein -
  J9537_RS01050 (J9537_01050) - 185305..188550 (+) 3246 WP_245001272.1 hypothetical protein -
  J9537_RS01055 (J9537_01055) - 188563..188922 (+) 360 WP_016178327.1 DUF6711 family protein -
  J9537_RS01060 (J9537_01060) - 188934..191543 (+) 2610 WP_218256410.1 gp58-like family protein -
  J9537_RS01065 (J9537_01065) - 191540..191698 (+) 159 WP_218256411.1 hypothetical protein -
  J9537_RS01070 (J9537_01070) - 191699..193240 (+) 1542 WP_218256412.1 hypothetical protein -
  J9537_RS01075 (J9537_01075) - 193307..193579 (+) 273 WP_195967677.1 hypothetical protein -
  J9537_RS01080 (J9537_01080) - 193613..193819 (+) 207 WP_218256413.1 phage holin -
  J9537_RS01085 (J9537_01085) - 193831..194835 (+) 1005 WP_218256414.1 N-acetylmuramoyl-L-alanine amidase -
  J9537_RS01095 (J9537_01095) - 195387..196034 (+) 648 WP_218256415.1 hypothetical protein -
  J9537_RS01100 (J9537_01100) - 196027..196449 (+) 423 WP_218256416.1 hypothetical protein -
  J9537_RS01105 (J9537_01105) - 196779..197048 (-) 270 WP_218256417.1 hypothetical protein -
  J9537_RS01110 (J9537_01110) - 197059..197463 (-) 405 WP_218256418.1 hypothetical protein -

Sequence


Protein


Download         Length: 145 a.a.        Molecular weight: 16208.73 Da        Isoelectric Point: 4.6968

>NTDB_id=556622 J9537_RS00885 WP_218256382.1 165043..165480(+) (ssb) [Enterococcus raffinosus strain F162_2]
MINNVVLVGRLTADPNLRYTASGTGVATFTLAVNRNFTNQDGNREADFINCVIWRKSAETLANYARKGTLIGVTGRIQTR
NYENQQGQRVYVTEVVADNFQLLESRSNNEKRQDDKSQQNTNTNNVESDPFNGSSIDIGDDDLPF

Nucleotide


Download         Length: 438 bp        

>NTDB_id=556622 J9537_RS00885 WP_218256382.1 165043..165480(+) (ssb) [Enterococcus raffinosus strain F162_2]
ATGATAAACAATGTGGTTCTAGTAGGAAGGTTAACGGCTGATCCAAACCTACGATATACAGCGAGCGGCACCGGGGTTGC
TACATTCACTCTAGCAGTGAACAGAAATTTCACGAATCAAGATGGAAATCGAGAAGCTGACTTCATCAATTGTGTGATTT
GGCGTAAGTCTGCAGAGACACTAGCGAACTATGCACGAAAAGGAACATTGATTGGTGTAACAGGACGTATCCAAACACGA
AATTATGAAAATCAGCAGGGCCAACGTGTCTATGTGACAGAAGTTGTTGCTGACAATTTCCAGTTATTGGAATCTCGTAG
TAACAATGAGAAACGTCAAGATGATAAGTCACAGCAGAATACAAATACAAACAATGTGGAGTCTGATCCATTCAATGGTT
CATCGATCGATATTGGTGACGACGATCTGCCGTTCTAG


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Latilactobacillus sakei subsp. sakei 23K

61.176

100

0.717

  ssbA Bacillus subtilis subsp. subtilis str. 168

52.907

100

0.628

  ssbB Streptococcus sobrinus strain NIDR 6715-7

46.207

100

0.462

  ssbB Bacillus subtilis subsp. subtilis str. 168

57.018

78.621

0.448

  ssbB/cilA Streptococcus pneumoniae TIGR4

43.448

100

0.434

  ssbB/cilA Streptococcus pneumoniae Rx1

42.759

100

0.428

  ssbB/cilA Streptococcus mitis NCTC 12261

42.759

100

0.428

  ssbB/cilA Streptococcus pneumoniae D39

42.759

100

0.428

  ssbB/cilA Streptococcus pneumoniae R6

42.759

100

0.428

  ssbB/cilA Streptococcus mitis SK321

42.759

100

0.428

  ssbA Streptococcus mutans UA159

42.069

100

0.421

  ssbB Lactococcus lactis subsp. cremoris KW2

46.903

77.931

0.366