Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   J4N37_RS14385 Genome accession   NZ_CP071855
Coordinates   3017893..3019626 (-) Length   577 a.a.
NCBI ID   WP_033008928.1    Uniprot ID   A0A7Y3ZC69
Organism   Vibrio sp. SCSIO 43153     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3012893..3024626
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J4N37_RS14365 (J4N37_14235) - 3013572..3014411 (-) 840 WP_252041238.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  J4N37_RS14370 (J4N37_14240) - 3014481..3016001 (-) 1521 WP_171306862.1 AAA family ATPase -
  J4N37_RS14375 (J4N37_14245) aroB 3016053..3017153 (-) 1101 WP_252041240.1 3-dehydroquinate synthase -
  J4N37_RS14380 (J4N37_14250) aroK 3017185..3017703 (-) 519 WP_010443904.1 shikimate kinase AroK -
  J4N37_RS14385 (J4N37_14255) pilQ 3017893..3019626 (-) 1734 WP_033008928.1 type IV pilus secretin PilQ family protein Machinery gene
  J4N37_RS14390 (J4N37_14260) pilP 3019664..3020179 (-) 516 WP_010443909.1 pilus assembly protein PilP Machinery gene
  J4N37_RS14395 (J4N37_14265) pilO 3020169..3020762 (-) 594 WP_071236468.1 type 4a pilus biogenesis protein PilO Machinery gene
  J4N37_RS14400 (J4N37_14270) pilN 3020759..3021343 (-) 585 WP_252041242.1 PilN domain-containing protein Machinery gene
  J4N37_RS14405 (J4N37_14275) pilM 3021327..3022343 (-) 1017 WP_252041244.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 63536.67 Da        Isoelectric Point: 4.5148

>NTDB_id=548428 J4N37_RS14385 WP_033008928.1 3017893..3019626(-) (pilQ) [Vibrio sp. SCSIO 43153]
MRQGYTLRYRKLFELLWLWSLLLMAPFVWAEESSSNALKSIDFRTNKDKEAVIIVELASPAAIVDVKRVQEGLSIDLLNT
SVEDNQLYLLDVKDFSTAVDSVEVFRDAATTRLLAHIEGDYTHDYRLTGRYLEVKVARLKPNEKVPDKSILEKEGKLISI
NFQDIPVRNVLQLIADYNDFNLVVSDSVEGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKEELDLREKQQLE
KLKLEEEIGELSSEIIKVNFAKASDIAQMINGEGNISMLSVRGSMTIDERTNSLLIRELPENIAVIREIVESLDIPVKQV
QIEARIVTVKEGNLDELGVRWGFTSINGNNMVGGSIENNLAAIGLYDEGGNAEDGSTVGIDDFLNVNLAAASTNASSIAF
QVAKLGSDTLLDLELSALQQESKAEIISSPRLVTTNKKPAYIEQGTEIPYLEASSSGATTITFKKAVLSLKVTPQITPDN
RLVLDLSVTQDRPGKVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSVSNTIDKVPLLGDLPLLGALFRRTYED
VGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=548428 J4N37_RS14385 WP_033008928.1 3017893..3019626(-) (pilQ) [Vibrio sp. SCSIO 43153]
ATGAGACAAGGATATACATTGAGATACAGGAAGTTATTTGAACTCTTGTGGTTATGGAGTTTGTTGTTAATGGCTCCTTT
TGTTTGGGCGGAAGAGTCGTCATCCAATGCATTGAAAAGTATTGATTTCAGGACTAACAAAGATAAAGAAGCGGTCATTA
TTGTTGAACTCGCTTCACCTGCAGCGATTGTAGATGTCAAAAGAGTTCAAGAAGGGCTTAGTATTGATTTGTTGAATACT
TCTGTTGAAGACAACCAGCTTTACTTGCTCGATGTTAAGGATTTCTCTACCGCAGTGGATAGCGTAGAAGTTTTTAGAGA
TGCAGCAACAACTAGATTGTTGGCGCACATTGAAGGTGACTATACACATGATTACAGGCTTACGGGGAGATATCTCGAAG
TAAAAGTTGCACGCTTAAAACCTAATGAAAAGGTGCCAGATAAAAGTATCCTTGAGAAGGAAGGCAAGCTCATCTCTATT
AACTTTCAAGATATACCGGTAAGAAATGTCTTGCAGTTGATCGCTGACTACAACGATTTCAACTTGGTGGTCTCGGATTC
TGTTGAGGGGAACTTAACGTTACGCTTGGATGGTGTGCCATGGCAGCAGGTACTAGATATTATTCTGCAGGTTAAGGGGC
TAGATAAACGCGTTGATGGCAACGTTATTTTAGTCGCACCAAAAGAAGAACTGGACTTACGTGAAAAGCAGCAATTGGAG
AAGTTGAAATTAGAAGAAGAAATAGGAGAGCTTTCTTCAGAAATAATCAAAGTTAATTTTGCTAAAGCTTCAGATATTGC
TCAAATGATCAATGGTGAAGGGAATATTAGCATGCTGTCTGTCCGTGGCAGTATGACCATTGATGAACGTACAAACTCTC
TACTTATCCGTGAACTACCAGAAAATATCGCAGTAATACGTGAGATTGTTGAGTCTCTTGATATTCCTGTTAAGCAAGTT
CAGATAGAGGCCAGAATTGTGACCGTGAAAGAGGGGAACCTAGATGAATTAGGTGTTCGCTGGGGCTTTACTTCTATTAA
TGGTAATAACATGGTTGGTGGTTCAATTGAGAATAACTTGGCTGCGATTGGCTTATACGACGAAGGTGGTAATGCTGAGG
ATGGAAGCACAGTTGGAATTGATGACTTTCTGAATGTCAATTTGGCAGCTGCATCGACAAATGCAAGCAGCATCGCTTTT
CAAGTCGCTAAATTGGGCTCTGACACTCTATTGGATTTGGAGCTTTCAGCTCTACAGCAAGAATCTAAGGCTGAAATCAT
CTCAAGTCCGCGTTTGGTAACGACAAACAAAAAGCCTGCCTATATCGAGCAAGGTACAGAAATTCCTTACTTAGAGGCTT
CCTCCAGTGGTGCAACGACCATTACTTTTAAAAAAGCAGTATTGAGTCTAAAAGTCACCCCCCAGATAACGCCCGATAAC
CGCTTAGTTCTTGATCTCAGCGTGACCCAGGATAGACCTGGTAAAGTTGTTAAAACTGGGACTGGAGAAGCGGTCGCGAT
TGATACACAGCGAATCGGCACGCAAGTGTTAGTAAATAATGGTGAAACCGTTGTTTTAGGCGGCATTTTCCAACATAGTG
TCAGTAATACAATTGACAAAGTTCCGCTGCTTGGAGATTTACCTTTGTTGGGCGCTCTATTTCGCCGTACTTATGAAGAT
GTAGGTAAGAGTGAGCTGTTGATCTTTGTTACGCCTAAGGTAGTAATTCAGTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(417-575)

Bacterial type II/III secretion system short domain (Secretin_N)

(254-319)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Y3ZC69

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

87.37

100

0.875
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

75.779

100

0.759
  pilQ Vibrio cholerae strain A1552

75.779

100

0.759
  pilQ Pseudomonas aeruginosa PAK

40.283

98.094

0.395
  pilQ Legionella pneumophila strain Lp02

40.468

96.36

0.39
  pilQ Legionella pneumophila strain ERS1305867

40.288

96.36

0.388
  comQ Acinetobacter baylyi ADP1

37.889

100

0.38
  pilQ Acinetobacter baumannii strain A118

36.364

100

0.374
  pilQ Acinetobacter baumannii D1279779

36.364

100

0.374