Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   J4N37_RS14405 Genome accession   NZ_CP071855
Coordinates   3021327..3022343 (-) Length   338 a.a.
NCBI ID   WP_252041244.1    Uniprot ID   -
Organism   Vibrio sp. SCSIO 43153     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3016327..3027343
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J4N37_RS14380 (J4N37_14250) aroK 3017185..3017703 (-) 519 WP_010443904.1 shikimate kinase AroK -
  J4N37_RS14385 (J4N37_14255) pilQ 3017893..3019626 (-) 1734 WP_033008928.1 type IV pilus secretin PilQ family protein Machinery gene
  J4N37_RS14390 (J4N37_14260) pilP 3019664..3020179 (-) 516 WP_010443909.1 pilus assembly protein PilP Machinery gene
  J4N37_RS14395 (J4N37_14265) pilO 3020169..3020762 (-) 594 WP_071236468.1 type 4a pilus biogenesis protein PilO Machinery gene
  J4N37_RS14400 (J4N37_14270) pilN 3020759..3021343 (-) 585 WP_252041242.1 PilN domain-containing protein Machinery gene
  J4N37_RS14405 (J4N37_14275) pilM 3021327..3022343 (-) 1017 WP_252041244.1 type IV pilus assembly protein PilM Machinery gene
  J4N37_RS14410 (J4N37_14280) - 3022484..3025051 (+) 2568 WP_010443931.1 PBP1A family penicillin-binding protein -
  J4N37_RS14415 (J4N37_14285) oxyR 3025130..3026038 (-) 909 WP_010443932.1 DNA-binding transcriptional regulator OxyR -
  J4N37_RS14420 (J4N37_14290) - 3026213..3026941 (+) 729 WP_010443933.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38241.63 Da        Isoelectric Point: 6.2468

>NTDB_id=548432 J4N37_RS14405 WP_252041244.1 3021327..3022343(-) (pilM) [Vibrio sp. SCSIO 43153]
MDKLTVAGIDISHNSLKAVILKPAKEGYSLLGYKEIVLNDAIVAENHTINHQEIVKTLKEIKKELPLLHRKAATSIPDSA
VISKSLQLDSQLEESEVEFAIMQAFSLQSPFPIEELSLDFVPNCDSKGNEVAGFRVFATRKEVVESRKQAIEKAGLTPIL
LDVHSHSQGAIWQLATERHPEKAQYCLLYVGERNCSISMFTAQGEFFYKEFSSGLEQVINNLQTSLEENASEQEKVERFN
HETADRVKRQLQLYSSMNGERDIRGIWLVGEGASTLMLAETLERQLQLDCELLNPFSLFEMKVPKRKRRNVDWQRFVTAA
GIAIRAIDWQRGSHVASR

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=548432 J4N37_RS14405 WP_252041244.1 3021327..3022343(-) (pilM) [Vibrio sp. SCSIO 43153]
ATGGATAAGTTAACAGTTGCGGGCATTGATATTAGTCACAATAGCCTAAAAGCCGTAATACTAAAGCCAGCAAAGGAGGG
GTACTCCTTGCTAGGTTATAAAGAAATAGTGCTAAACGACGCTATTGTCGCTGAAAACCATACAATAAATCATCAAGAAA
TTGTAAAGACCCTAAAAGAGATAAAAAAAGAACTACCACTATTACACCGAAAAGCGGCTACATCTATTCCAGATAGCGCT
GTAATTAGCAAAAGCTTACAGCTTGACTCTCAATTGGAAGAAAGTGAGGTTGAGTTTGCAATCATGCAGGCATTTTCTCT
TCAGTCTCCGTTTCCAATTGAGGAATTAAGTTTAGATTTTGTGCCGAACTGTGACAGCAAAGGAAATGAGGTTGCTGGTT
TTCGAGTCTTTGCTACTAGAAAAGAGGTGGTAGAAAGTCGAAAACAGGCAATTGAAAAGGCTGGCCTGACACCTATTTTG
CTTGACGTTCACTCTCACTCTCAGGGAGCAATATGGCAATTGGCGACAGAGCGGCATCCTGAAAAAGCGCAATACTGTTT
GCTTTATGTGGGAGAAAGAAACTGTTCTATTTCCATGTTTACCGCTCAAGGTGAGTTCTTTTATAAGGAGTTTTCATCAG
GCTTAGAGCAAGTTATCAATAACCTTCAAACATCTCTTGAGGAGAATGCGAGCGAACAAGAGAAAGTGGAAAGGTTTAAC
CATGAAACGGCAGATAGGGTAAAGAGACAGCTTCAACTTTACAGCTCAATGAATGGTGAACGAGATATTCGTGGTATTTG
GCTTGTCGGTGAAGGCGCATCCACTTTAATGCTCGCAGAAACGTTGGAGCGTCAGTTACAACTGGATTGTGAATTGTTGA
ATCCATTTAGTCTTTTTGAAATGAAGGTTCCGAAGCGTAAAAGAAGAAATGTTGACTGGCAGCGTTTTGTAACTGCGGCT
GGTATAGCAATAAGAGCTATTGACTGGCAAAGGGGGAGCCATGTTGCATCCCGTTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

59.412

100

0.598
  pilM Vibrio cholerae strain A1552

45.64

100

0.465