Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   J4N37_RS14395 Genome accession   NZ_CP071855
Coordinates   3020169..3020762 (-) Length   197 a.a.
NCBI ID   WP_071236468.1    Uniprot ID   A0A2K7SSG0
Organism   Vibrio sp. SCSIO 43153     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3015169..3025762
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J4N37_RS14375 (J4N37_14245) aroB 3016053..3017153 (-) 1101 WP_252041240.1 3-dehydroquinate synthase -
  J4N37_RS14380 (J4N37_14250) aroK 3017185..3017703 (-) 519 WP_010443904.1 shikimate kinase AroK -
  J4N37_RS14385 (J4N37_14255) pilQ 3017893..3019626 (-) 1734 WP_033008928.1 type IV pilus secretin PilQ family protein Machinery gene
  J4N37_RS14390 (J4N37_14260) pilP 3019664..3020179 (-) 516 WP_010443909.1 pilus assembly protein PilP Machinery gene
  J4N37_RS14395 (J4N37_14265) pilO 3020169..3020762 (-) 594 WP_071236468.1 type 4a pilus biogenesis protein PilO Machinery gene
  J4N37_RS14400 (J4N37_14270) pilN 3020759..3021343 (-) 585 WP_252041242.1 PilN domain-containing protein Machinery gene
  J4N37_RS14405 (J4N37_14275) pilM 3021327..3022343 (-) 1017 WP_252041244.1 type IV pilus assembly protein PilM Machinery gene
  J4N37_RS14410 (J4N37_14280) - 3022484..3025051 (+) 2568 WP_010443931.1 PBP1A family penicillin-binding protein -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 22814.34 Da        Isoelectric Point: 5.7559

>NTDB_id=548430 J4N37_RS14395 WP_071236468.1 3020169..3020762(-) (pilO) [Vibrio sp. SCSIO 43153]
MSNWQELELDEITDWPLLPQSLVVFLLVIVLLAGGYWAFVKPDKQRLNSLIAEEQKLKVMLKIKANKVAALPKLQAQLDE
LTHRYEYLLQQLPAQKELATMLASVNELGIDNNLTFTRIDWGKKQSEQFLYRLPLNIELTGAFHDIGSFAQAIATLPRII
TFKDVTWQRVSQESETLHFRVRANTYQFKPEEKQDES

Nucleotide


Download         Length: 594 bp        

>NTDB_id=548430 J4N37_RS14395 WP_071236468.1 3020169..3020762(-) (pilO) [Vibrio sp. SCSIO 43153]
ATGAGCAACTGGCAAGAACTAGAATTAGATGAAATAACAGATTGGCCTCTATTACCCCAGTCTTTGGTCGTTTTTTTATT
GGTAATAGTTCTACTAGCAGGGGGCTATTGGGCTTTTGTTAAGCCGGATAAACAGCGTTTGAACTCGTTAATCGCCGAAG
AGCAGAAACTCAAAGTAATGTTGAAAATCAAAGCCAATAAGGTCGCAGCATTACCCAAACTGCAAGCTCAATTGGATGAA
CTGACTCATCGATATGAATATCTATTGCAGCAATTACCAGCACAGAAAGAGCTGGCCACAATGCTTGCTTCTGTCAACGA
ACTTGGCATCGATAATAATCTGACTTTCACACGGATCGATTGGGGGAAGAAACAATCTGAACAATTTTTGTATAGGCTCC
CATTGAATATCGAACTTACTGGCGCATTTCATGATATTGGGAGCTTCGCACAAGCGATAGCAACGCTTCCTCGCATCATT
ACATTTAAGGATGTGACTTGGCAGAGAGTCAGTCAAGAGAGTGAAACCTTACATTTTCGAGTTAGAGCCAATACTTATCA
GTTTAAGCCAGAGGAAAAACAAGATGAAAGCTAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(48-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2K7SSG0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

78.421

96.447

0.756
  pilO Vibrio cholerae strain A1552

65.445

96.954

0.635