Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilA1   Type   Machinery gene
Locus tag   J1M35_RS20840 Genome accession   NZ_CP071796
Coordinates   252043..252468 (+) Length   141 a.a.
NCBI ID   WP_208009328.1    Uniprot ID   A0A975CG45
Organism   Ottowia testudinis strain 27C     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 247043..257468
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J1M35_RS01205 (J1M35_01205) - 247162..247872 (+) 711 WP_208009324.1 TerC family protein -
  J1M35_RS01210 (J1M35_01210) - 247951..248736 (+) 786 WP_208009325.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  J1M35_RS01215 (J1M35_01215) - 248824..249453 (+) 630 WP_208009326.1 FHA domain-containing protein -
  J1M35_RS01220 (J1M35_01220) - 249450..251723 (+) 2274 WP_208009327.1 adenylate/guanylate cyclase domain-containing protein -
  J1M35_RS20840 (J1M35_01225) pilA/pilA1 252043..252468 (+) 426 WP_208009328.1 pilin Machinery gene
  J1M35_RS01230 (J1M35_01230) - 252570..254363 (+) 1794 WP_208009329.1 tetratricopeptide repeat protein -
  J1M35_RS01235 (J1M35_01235) - 254464..255915 (+) 1452 WP_208009330.1 hypothetical protein -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 14499.69 Da        Isoelectric Point: 9.0355

>NTDB_id=547818 J1M35_RS20840 WP_208009328.1 252043..252468(+) (pilA/pilA1) [Ottowia testudinis strain 27C]
MKSLQKGFTLIELMIVVAIIGILAAIALPAYQDYVARAQASEALKATAGLQADIGVNYADSNSLAPTSTMSAQASSLGGK
YFSVGGVSLGSTGQIMVAFNNGALASQGMTITPTAANGQIQYWICSGLSKPAHIPSTCRQR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=547818 J1M35_RS20840 WP_208009328.1 252043..252468(+) (pilA/pilA1) [Ottowia testudinis strain 27C]
ATGAAATCGCTCCAAAAAGGTTTTACCCTGATCGAATTGATGATTGTCGTGGCGATCATCGGTATTCTGGCTGCCATCGC
TCTGCCAGCATATCAGGATTATGTAGCACGCGCCCAGGCCTCTGAAGCACTGAAGGCGACCGCAGGCCTGCAGGCGGACA
TTGGCGTTAACTATGCGGACAGCAACAGCCTTGCGCCTACTTCTACAATGAGCGCTCAAGCCAGTTCGCTGGGCGGTAAG
TACTTCTCTGTTGGGGGTGTATCGCTTGGCAGTACGGGGCAAATTATGGTTGCATTTAACAATGGTGCGCTCGCCAGCCA
GGGCATGACCATTACACCTACTGCTGCTAATGGTCAAATCCAATACTGGATTTGCAGCGGCCTGTCAAAGCCCGCCCATA
TTCCTTCAACCTGCCGTCAACGTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(4-28)

Pilin (bacterial filament) (Pilin)

(36-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilA1 Eikenella corrodens VA1

53.595

100

0.582
  pilE Neisseria gonorrhoeae strain FA1090

37.037

100

0.426
  pilE Neisseria gonorrhoeae MS11

36.42

100

0.418
  pilA2 Legionella pneumophila strain ERS1305867

42.143

99.291

0.418
  pilA Acinetobacter nosocomialis M2

44.531

90.78

0.404
  comP Acinetobacter baylyi ADP1

39.583

100

0.404
  pilA2 Legionella pneumophila str. Paris

40.288

98.582

0.397
  pilA Pseudomonas aeruginosa PAK

35.526

100

0.383
  pilA Vibrio cholerae C6706

36.62

100

0.369
  pilA Vibrio cholerae strain A1552

36.62

100

0.369
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

36.62

100

0.369