Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   J0J33_RS03040 Genome accession   NZ_CP071295
Coordinates   605830..606774 (+) Length   314 a.a.
NCBI ID   WP_173816876.1    Uniprot ID   -
Organism   Lactococcus sp. LG1267     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 600830..611774
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J0J33_RS10735 - 601452..603695 (-) 2244 WP_242526937.1 GH25 family lysozyme -
  J0J33_RS03030 (J0J33_03030) guaB 603861..605342 (+) 1482 WP_003133714.1 IMP dehydrogenase -
  J0J33_RS03035 (J0J33_03035) rpsU 605468..605644 (+) 177 WP_003134539.1 30S ribosomal protein S21 -
  J0J33_RS03040 (J0J33_03040) comGA 605830..606774 (+) 945 WP_173816876.1 competence type IV pilus ATPase ComGA Machinery gene
  J0J33_RS03045 (J0J33_03045) comGB/cglB 606713..607732 (+) 1020 WP_256440196.1 competence type IV pilus assembly protein ComGB Machinery gene
  J0J33_RS03050 (J0J33_03050) comYC 607751..608056 (+) 306 WP_040087209.1 competence type IV pilus major pilin ComGC Machinery gene
  J0J33_RS10740 comGD 608031..608447 (+) 417 WP_019293010.1 competence type IV pilus minor pilin ComGD Machinery gene
  J0J33_RS10870 (J0J33_03055) comGE 608479..608712 (+) 234 WP_019293009.1 competence type IV pilus minor pilin ComGE -
  J0J33_RS03060 (J0J33_03060) comGF 608699..609133 (+) 435 WP_242526938.1 competence type IV pilus minor pilin ComGF -
  J0J33_RS03065 (J0J33_03065) - 609365..610210 (+) 846 WP_019336001.1 metal ABC transporter solute-binding protein, Zn/Mn family -
  J0J33_RS03070 (J0J33_03070) - 610281..611165 (-) 885 WP_086582775.1 RluA family pseudouridine synthase -

Sequence

Protein


Download         Length: 314 a.a.        Molecular weight: 35861.27 Da        Isoelectric Point: 7.2081

>NTDB_id=544707 J0J33_RS03040 WP_173816876.1 605830..606774(+) (comGA) [Lactococcus sp. LG1267]
MIQVLAKNLLQKAVDQMVHDLYLVALEGKYFLYFRTATERRFERELTFEQGQALIAHMKFLSGMNLGESRRVQLGACTYI
LDKGEQRLRLSTVGDFHGQESLVIRLLHHQQNQLHFWNSEIFNTIIGGRGLYLFSGPVGSGKTSLMYKFAREHFKNQQVI
CIEDPVELVETEFLQLQVNKVIGNDYDALIKLSLRHRPDLLIVGEIRDSQTAKAVLRASLTGYTVFSTVHARSISGVIAR
LKELGLSDWELQSSLQRVIYQRLIAGKGLLVCEKEKFEEWKPDEWNDQIDQLVADGFISAIAAAREKIEFSKAD

Nucleotide


Download         Length: 945 bp        

>NTDB_id=544707 J0J33_RS03040 WP_173816876.1 605830..606774(+) (comGA) [Lactococcus sp. LG1267]
ATGATACAGGTTTTGGCAAAAAACCTTTTACAAAAAGCAGTAGATCAGATGGTGCACGATCTTTATTTAGTAGCACTAGA
AGGAAAGTATTTTCTCTATTTTCGTACAGCAACAGAAAGGAGGTTTGAAAGAGAACTCACCTTTGAGCAAGGGCAAGCAC
TGATTGCTCATATGAAATTTTTATCTGGTATGAATCTAGGAGAAAGTAGACGAGTACAGTTGGGGGCTTGCACTTATATT
TTAGATAAAGGTGAACAGCGCTTACGTTTATCTACTGTCGGTGATTTTCATGGTCAAGAAAGTTTGGTTATTCGACTCTT
GCATCATCAACAAAACCAACTTCATTTTTGGAATTCAGAGATCTTTAATACTATTATTGGGGGAAGAGGCCTTTACCTTT
TTTCAGGTCCTGTAGGTTCAGGTAAGACCAGTCTTATGTACAAATTTGCAAGGGAGCATTTTAAAAATCAACAAGTTATT
TGTATAGAAGATCCTGTGGAGTTAGTAGAAACTGAATTTTTACAACTTCAAGTCAATAAAGTAATAGGGAATGATTATGA
TGCCTTGATTAAGCTTTCGCTTCGCCACAGACCTGATTTACTTATTGTTGGTGAAATTAGAGATAGTCAAACGGCAAAAG
CTGTTCTCCGTGCAAGTTTGACTGGGTATACAGTATTCTCTACTGTTCATGCACGCTCAATATCAGGTGTAATTGCACGC
TTAAAAGAACTTGGATTAAGTGATTGGGAGCTACAATCCAGTCTTCAGCGGGTGATTTATCAACGATTAATTGCAGGAAA
GGGATTGCTTGTTTGTGAAAAGGAAAAGTTTGAAGAATGGAAACCAGACGAATGGAATGACCAGATCGATCAGTTGGTTG
CAGATGGATTTATCTCAGCTATTGCAGCTGCGCGCGAAAAAATTGAGTTTAGCAAAGCAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-264)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Lactococcus lactis subsp. cremoris KW2

60.51

100

0.605
  comYA Streptococcus gordonii str. Challis substr. CH1

53.651

100

0.538
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

53.035

99.682

0.529
  comGA/cglA/cilD Streptococcus pneumoniae R6

52.716

99.682

0.525
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

52.716

99.682

0.525
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

52.716

99.682

0.525
  comGA/cglA/cilD Streptococcus pneumoniae D39

52.716

99.682

0.525
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

52.564

99.363

0.522
  comYA Streptococcus mutans UA159

50.949

100

0.513
  comYA Streptococcus mutans UA140

50.949

100

0.513