Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   J0J33_RS03045 Genome accession   NZ_CP071295
Coordinates   606713..607732 (+) Length   339 a.a.
NCBI ID   WP_256440196.1    Uniprot ID   -
Organism   Lactococcus sp. LG1267     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 601713..612732
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J0J33_RS03030 (J0J33_03030) guaB 603861..605342 (+) 1482 WP_003133714.1 IMP dehydrogenase -
  J0J33_RS03035 (J0J33_03035) rpsU 605468..605644 (+) 177 WP_003134539.1 30S ribosomal protein S21 -
  J0J33_RS03040 (J0J33_03040) comGA 605830..606774 (+) 945 WP_173816876.1 competence type IV pilus ATPase ComGA Machinery gene
  J0J33_RS03045 (J0J33_03045) comGB/cglB 606713..607732 (+) 1020 WP_256440196.1 competence type IV pilus assembly protein ComGB Machinery gene
  J0J33_RS03050 (J0J33_03050) comYC 607751..608056 (+) 306 WP_040087209.1 competence type IV pilus major pilin ComGC Machinery gene
  J0J33_RS10740 comGD 608031..608447 (+) 417 WP_019293010.1 competence type IV pilus minor pilin ComGD Machinery gene
  J0J33_RS10870 (J0J33_03055) comGE 608479..608712 (+) 234 WP_019293009.1 competence type IV pilus minor pilin ComGE -
  J0J33_RS03060 (J0J33_03060) comGF 608699..609133 (+) 435 WP_242526938.1 competence type IV pilus minor pilin ComGF -
  J0J33_RS03065 (J0J33_03065) - 609365..610210 (+) 846 WP_019336001.1 metal ABC transporter solute-binding protein, Zn/Mn family -
  J0J33_RS03070 (J0J33_03070) - 610281..611165 (-) 885 WP_086582775.1 RluA family pseudouridine synthase -

Sequence

Protein


Download         Length: 339 a.a.        Molecular weight: 39540.17 Da        Isoelectric Point: 9.3792

>NTDB_id=544708 J0J33_RS03045 WP_256440196.1 606713..607732(+) (comGB/cglB) [Lactococcus sp. LG1267]
MDLSQLLQLRAKKLSLAKQIKLIQLMNNLFKSGFHLSEIIDFLERSGLTEAYFISKMREGLLNGQSFSGILAKLNFYEDV
VTQLSLAESHGNVEYTLGLIEEKLIRVLNIRKKLVQVATYPIVLLAFLIFIMLGLKNYLLPQLEENKGLATYAIQNLPTL
FLGWLFSLFVFFYLCRYYWKKKTALEALRLMVKIPFVGRFVQLYLTAYFAREWGNLIAQAVDLRQICFIMQEQKSRIFKE
FGFELLQILDSGQKFEDTLRFYPIFTKELSLIVEYGELKSKLGKELMIFSEENWSLFFERMERAMQLIQPIVFLLVALLI
ILIYAAMLLPIYSNMGNLI

Nucleotide


Download         Length: 1020 bp        

>NTDB_id=544708 J0J33_RS03045 WP_256440196.1 606713..607732(+) (comGB/cglB) [Lactococcus sp. LG1267]
ATGGATTTATCTCAGCTATTGCAGCTGCGCGCGAAAAAATTGAGTTTAGCAAAGCAGATTAAGCTCATTCAACTCATGAA
TAATCTTTTCAAAAGTGGCTTTCACCTCTCTGAAATTATTGATTTCCTTGAACGATCGGGTCTTACAGAAGCATACTTTA
TTTCCAAAATGCGTGAAGGTTTACTTAATGGGCAAAGTTTCTCTGGTATTTTGGCGAAGCTAAATTTTTATGAGGATGTT
GTGACCCAGCTCTCTTTGGCTGAGTCTCATGGGAATGTTGAGTATACTTTAGGATTGATTGAAGAAAAACTGATACGTGT
TTTGAACATCCGCAAAAAGCTTGTCCAAGTTGCCACTTATCCGATTGTTCTTCTGGCATTTCTAATCTTTATTATGCTTG
GATTGAAAAATTATTTACTGCCCCAACTGGAAGAAAACAAAGGATTAGCAACTTATGCCATACAGAATCTTCCGACCCTC
TTTTTAGGGTGGCTCTTCAGTTTGTTCGTTTTCTTTTATCTGTGCAGATACTATTGGAAGAAAAAAACTGCTTTAGAAGC
GCTGAGGCTAATGGTCAAAATACCTTTTGTTGGCCGTTTTGTCCAGCTTTATTTGACAGCTTATTTTGCAAGAGAATGGG
GAAACTTAATTGCTCAAGCTGTCGATTTACGTCAGATTTGTTTTATTATGCAAGAACAAAAAAGTCGAATTTTCAAAGAA
TTCGGTTTTGAGCTTCTTCAAATATTAGATAGTGGTCAGAAGTTTGAAGATACTCTTCGATTTTACCCCATATTTACCAA
GGAGCTCTCATTAATTGTGGAATATGGTGAACTAAAGTCCAAGCTAGGGAAAGAGTTAATGATTTTTTCTGAAGAAAACT
GGTCGCTCTTTTTTGAAAGGATGGAAAGAGCAATGCAACTTATCCAGCCTATCGTTTTCTTGCTCGTGGCGCTGTTGATT
ATTTTAATTTATGCGGCAATGCTACTTCCTATATATAGCAATATGGGAAACTTGATTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(23-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-330)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus mitis NCTC 12261

52.836

98.82

0.522
  comYB Streptococcus gordonii str. Challis substr. CH1

52.695

98.525

0.519
  comGB Lactococcus lactis subsp. cremoris KW2

52.381

99.115

0.519
  comGB/cglB Streptococcus pneumoniae R6

52.537

98.82

0.519
  comGB/cglB Streptococcus pneumoniae TIGR4

52.537

98.82

0.519
  comGB/cglB Streptococcus mitis SK321

52.537

98.82

0.519
  comGB/cglB Streptococcus pneumoniae Rx1

52.537

98.82

0.519
  comGB/cglB Streptococcus pneumoniae D39

52.537

98.82

0.519
  comYB Streptococcus mutans UA140

52.548

92.625

0.487
  comYB Streptococcus mutans UA159

52.229

92.625

0.484