Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AMAD1_RS15215 Genome accession   NC_019393
Coordinates   3443293..3444405 (-) Length   370 a.a.
NCBI ID   WP_012519492.1    Uniprot ID   F2GD64
Organism   Alteromonas mediterranea DE1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3438293..3449405
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AMAD1_RS15190 (amad1_15260) - 3439851..3440579 (+) 729 WP_012519487.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AMAD1_RS15195 (amad1_15265) gshB 3440799..3441749 (+) 951 WP_012519488.1 glutathione synthase -
  AMAD1_RS15200 (amad1_15270) - 3441861..3442427 (+) 567 WP_041703495.1 YqgE/AlgH family protein -
  AMAD1_RS15205 (amad1_15275) ruvX 3442420..3442851 (+) 432 WP_012519490.1 Holliday junction resolvase RuvX -
  AMAD1_RS15210 (amad1_15280) - 3442941..3443267 (-) 327 WP_015067844.1 DUF2007 domain-containing protein -
  AMAD1_RS15215 (amad1_15285) pilU 3443293..3444405 (-) 1113 WP_012519492.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AMAD1_RS15220 (amad1_15290) pilT 3444414..3445457 (-) 1044 WP_012519493.1 type IV pilus twitching motility protein PilT Machinery gene
  AMAD1_RS15225 (amad1_15295) - 3445485..3446183 (+) 699 WP_012519494.1 YggS family pyridoxal phosphate-dependent enzyme -
  AMAD1_RS15230 (amad1_15300) proC 3446235..3447056 (+) 822 WP_012519495.1 pyrroline-5-carboxylate reductase -
  AMAD1_RS15235 (amad1_15305) - 3447077..3447613 (+) 537 WP_012519496.1 YggT family protein -
  AMAD1_RS15240 (amad1_15310) - 3447697..3448137 (+) 441 WP_015067845.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40798.64 Da        Isoelectric Point: 6.0479

>NTDB_id=54386 AMAD1_RS15215 WP_012519492.1 3443293..3444405(-) (pilU) [Alteromonas mediterranea DE1]
MLDSLLQKMVDTNASDLFVTAGFPVSAKINGKLTPVTDSATSEEASLALVHEAMTDKQRDEFHTSKECNFAIVRDGIGRF
RCSAFWQRDQAGMVIRRIVTQIPKADDLGLPRVLKEIIMSKRGLVLFVGGTGTGKSTSLAALIGHRNENSHGHILTIEDP
IEFVHEHKNCVITQREVGIDTQSFDDALKSSLRQAPDVILIGEIRSMETMEYAMSFADTGHLCVATLHANNANQAIERIM
HLAPKDQHDKLRFDLSLNIRAIVAQQLVPTPDGKGRVAAIEILLNSPLVRDLIQRNEIGALKEAMKKGKEQGMQSFDMAL
YDLYKAGRIDMDQALHHADSPNDLRLMIKLDTSDGSSLGTLSNVSIDMDD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=54386 AMAD1_RS15215 WP_012519492.1 3443293..3444405(-) (pilU) [Alteromonas mediterranea DE1]
ATGCTAGATTCACTACTTCAAAAAATGGTTGATACCAACGCGTCAGATTTATTTGTCACTGCGGGCTTTCCGGTTAGCGC
TAAAATAAATGGTAAACTTACCCCTGTCACTGATAGCGCAACGTCAGAAGAGGCATCACTCGCGCTGGTTCATGAAGCCA
TGACCGATAAGCAGCGAGACGAGTTTCATACGTCGAAGGAATGCAACTTTGCTATCGTGCGTGATGGCATTGGTCGTTTT
CGTTGTTCTGCGTTTTGGCAACGAGACCAAGCGGGTATGGTTATCCGTCGTATTGTGACGCAAATACCGAAAGCCGATGA
CTTGGGTCTACCCCGAGTATTAAAAGAAATTATCATGTCTAAGCGCGGTCTTGTTCTATTCGTAGGTGGGACAGGTACGG
GTAAATCAACCTCGCTAGCTGCACTCATTGGGCATAGGAATGAAAACTCTCACGGGCATATACTGACCATTGAGGACCCT
ATTGAATTTGTACACGAGCATAAAAACTGTGTGATTACACAGCGTGAGGTGGGTATTGATACCCAGTCATTCGACGATGC
GCTTAAGAGCTCGTTACGACAAGCGCCAGACGTTATTCTAATTGGTGAGATTCGGTCTATGGAAACCATGGAATATGCCA
TGTCGTTCGCCGATACGGGCCACCTGTGTGTGGCAACGCTGCATGCGAACAACGCGAACCAAGCCATAGAGCGTATTATG
CACTTAGCGCCTAAAGATCAGCACGACAAGCTGCGCTTTGATTTAAGCTTGAACATTCGTGCTATTGTTGCCCAGCAACT
GGTGCCGACGCCAGACGGTAAAGGGCGTGTGGCTGCCATCGAGATACTGCTTAATTCACCATTAGTGCGTGACCTTATTC
AGCGCAATGAAATAGGCGCGTTGAAAGAAGCCATGAAAAAAGGCAAAGAGCAGGGCATGCAAAGTTTTGATATGGCGCTC
TACGACCTTTACAAGGCCGGCCGAATTGACATGGATCAGGCGCTGCATCATGCTGACTCTCCGAATGATTTACGCTTAAT
GATTAAGCTGGATACCAGCGATGGCTCAAGCCTAGGTACGCTGTCTAATGTGTCGATTGATATGGACGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F2GD64

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.377

100

0.605
  pilU Acinetobacter baylyi ADP1

57.26

98.649

0.565
  pilU Vibrio cholerae strain A1552

55.435

99.459

0.551
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.765

91.892

0.384
  pilT Acinetobacter nosocomialis M2

39.65

92.703

0.368
  pilT Acinetobacter baumannii D1279779

39.65

92.703

0.368
  pilT Acinetobacter baumannii strain A118

39.65

92.703

0.368
  pilT Pseudomonas stutzeri DSM 10701

39.067

92.703

0.362
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.729

88.919

0.362
  pilT Vibrio cholerae strain A1552

40.729

88.919

0.362

Multiple sequence alignment