Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AMAD1_RS15220 Genome accession   NC_019393
Coordinates   3444414..3445457 (-) Length   347 a.a.
NCBI ID   WP_012519493.1    Uniprot ID   F2GD65
Organism   Alteromonas mediterranea DE1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3439414..3450457
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AMAD1_RS15190 (amad1_15260) - 3439851..3440579 (+) 729 WP_012519487.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AMAD1_RS15195 (amad1_15265) gshB 3440799..3441749 (+) 951 WP_012519488.1 glutathione synthase -
  AMAD1_RS15200 (amad1_15270) - 3441861..3442427 (+) 567 WP_041703495.1 YqgE/AlgH family protein -
  AMAD1_RS15205 (amad1_15275) ruvX 3442420..3442851 (+) 432 WP_012519490.1 Holliday junction resolvase RuvX -
  AMAD1_RS15210 (amad1_15280) - 3442941..3443267 (-) 327 WP_015067844.1 DUF2007 domain-containing protein -
  AMAD1_RS15215 (amad1_15285) pilU 3443293..3444405 (-) 1113 WP_012519492.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AMAD1_RS15220 (amad1_15290) pilT 3444414..3445457 (-) 1044 WP_012519493.1 type IV pilus twitching motility protein PilT Machinery gene
  AMAD1_RS15225 (amad1_15295) - 3445485..3446183 (+) 699 WP_012519494.1 YggS family pyridoxal phosphate-dependent enzyme -
  AMAD1_RS15230 (amad1_15300) proC 3446235..3447056 (+) 822 WP_012519495.1 pyrroline-5-carboxylate reductase -
  AMAD1_RS15235 (amad1_15305) - 3447077..3447613 (+) 537 WP_012519496.1 YggT family protein -
  AMAD1_RS15240 (amad1_15310) - 3447697..3448137 (+) 441 WP_015067845.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38424.10 Da        Isoelectric Point: 6.4436

>NTDB_id=54387 AMAD1_RS15220 WP_012519493.1 3444414..3445457(-) (pilT) [Alteromonas mediterranea DE1]
MDITELLAFSVKNNASDLHLSAGLPPIIRVDGEMRKLNVPALDHKQVHSLVYEIMNDMQRKEYEENLETDFSFEVNGLSR
FRVNAFVQNRGAAAVLRTIPSKVLTLDDLGAPEIFKEIINQPTGIVLVTGATGSGKSTTLAAMVDHINTHKREHILTIED
PIEFVHDNKLSLVNQREVHRDTHSFNNALRSALREDPDVILVGELRDLETIRLAISAAETGHLVFGTLHTNSAPKTIDRI
IDVFPAEEKAMVRSMLSESLRAVISQTLLKKVGGGRVAAHEIMVGIPAIRNLIREDKVPQMYSVIQTGQAHGMQTMDQCL
QRLVAMGVISKEDAAAKSVDKQSMNNF

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=54387 AMAD1_RS15220 WP_012519493.1 3444414..3445457(-) (pilT) [Alteromonas mediterranea DE1]
GTGGATATTACCGAACTTTTGGCTTTCAGTGTTAAGAATAACGCCTCAGATTTACACTTATCAGCGGGCCTACCACCAAT
TATTCGTGTAGATGGCGAGATGCGTAAACTGAACGTGCCTGCCCTCGATCACAAGCAAGTACATAGTCTCGTTTACGAGA
TTATGAACGACATGCAGCGCAAAGAATACGAAGAAAACTTAGAAACCGATTTTTCTTTTGAAGTGAATGGGTTATCTCGT
TTTCGTGTCAATGCCTTTGTTCAGAACCGCGGTGCCGCCGCTGTTCTGCGTACTATCCCCAGCAAAGTATTAACGTTAGA
CGACCTTGGTGCCCCGGAAATATTTAAAGAGATCATCAATCAGCCTACAGGTATTGTGCTGGTAACCGGTGCCACAGGTT
CGGGTAAAAGTACTACCTTGGCGGCCATGGTCGACCATATTAATACGCACAAACGCGAACATATTCTGACCATTGAAGAC
CCCATCGAATTCGTGCACGACAATAAGCTCAGTTTGGTAAACCAGCGTGAAGTGCATCGCGATACCCACTCTTTTAACAA
TGCATTGCGTTCCGCGTTACGTGAAGACCCAGACGTTATTCTTGTCGGTGAGCTGCGTGACCTTGAAACCATTCGCCTTG
CTATCTCGGCCGCGGAAACAGGTCACTTAGTATTTGGTACGCTACATACTAACTCAGCGCCTAAAACTATTGACCGGATT
ATTGACGTTTTTCCTGCAGAAGAAAAAGCCATGGTGCGCTCTATGTTATCTGAGTCTTTACGCGCGGTTATTTCTCAAAC
ACTGCTAAAGAAAGTAGGGGGAGGCCGAGTGGCGGCTCACGAAATCATGGTAGGTATCCCAGCTATTCGTAACCTCATTC
GCGAAGATAAAGTGCCTCAGATGTATTCGGTTATCCAAACCGGTCAGGCCCACGGTATGCAAACCATGGATCAGTGCTTA
CAGCGCCTGGTCGCCATGGGAGTTATAAGTAAAGAAGACGCGGCTGCAAAGTCAGTGGATAAACAGTCGATGAACAATTT
CTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F2GD65

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

75.872

99.135

0.752
  pilT Acinetobacter baumannii strain A118

75.872

99.135

0.752
  pilT Acinetobacter nosocomialis M2

75.581

99.135

0.749
  pilT Pseudomonas stutzeri DSM 10701

75.072

99.424

0.746
  pilT Pseudomonas aeruginosa PAK

75.444

97.406

0.735
  pilT Acinetobacter baylyi ADP1

73.837

99.135

0.732
  pilT Legionella pneumophila strain Lp02

72.141

98.271

0.709
  pilT Legionella pneumophila strain ERS1305867

72.141

98.271

0.709
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.404

97.118

0.703
  pilT Vibrio cholerae strain A1552

72.404

97.118

0.703
  pilT Neisseria meningitidis 8013

65.318

99.712

0.651
  pilT Neisseria gonorrhoeae MS11

65.029

99.712

0.648
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.603

98.847

0.51
  pilU Acinetobacter baylyi ADP1

41.716

97.406

0.406
  pilU Pseudomonas stutzeri DSM 10701

41.791

96.542

0.403
  pilU Vibrio cholerae strain A1552

41.791

96.542

0.403

Multiple sequence alignment