Detailed information NTG0000651

Experimentally validated

Overview

• Name: recO
• Type: Machinery gene
• Locus tag: SPR_RS00200
• Genome accession: NC_003098
• Coordinates: 36977..37747 (+)
• Length: 256 a.a.
• NCBI ID: WP_000616166.1
• Uniprot ID: Q8DRN4
• Organism: Streptococcus pneumoniae R6
• Function: homologous recombination Homologous recombination

Function

A ∼1.45-fold drop in transformation was observed with total chromosomal DNA in recFOR mutants. Transformation with chromosomal DNA stimulated unexpectedly high frequency (>30% of cells) formation of chromosome dimers as an intermediate in the generation of tandem duplications, and that RecFOR were crucial for dimer resolution. The site-specific recombinase XerS was also crucial for dimer resolution.

Genomic Context

Location: 31977..42747

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SPR_RS00185 (spr0033)	-	34024..34461 (+)	438	WP_000076479.1	CoA-binding protein	-
SPR_RS00190 (spr0034)	-	34652..35662 (-)	1011	WP_000009141.1	YeiH family protein	-
SPR_RS00195 (spr0035)	-	35811..36980 (+)	1170	WP_000366378.1	pyridoxal phosphate-dependent aminotransferase	-
SPR_RS00200 (spr0036)	recO	36977..37747 (+)	771	WP_000616166.1	DNA repair protein RecO	Machinery gene
SPR_RS00205 (spr0037)	plsX	37744..38736 (+)	993	WP_000717456.1	phosphate acyltransferase PlsX	-
SPR_RS00210 (spr0038)	-	38742..38975 (+)	234	WP_000136447.1	acyl carrier protein	-
SPR_RS00215 (spr0039)	-	39012..39311 (+)	300	Protein_34	transposase family protein	-
SPR_RS00220 (spr0040)	blpU	39514..39744 (+)	231	WP_001093075.1	bacteriocin-like peptide BlpU	-
SPR_RS10920	-	39747..39872 (+)	126	WP_000346297.1	PncF family bacteriocin immunity protein	-

Sequence

Protein

• Length: 256 a.a.
• Molecular weight: 29818.32 Da
• Isoelectric Point: 5.3615

>NTDB_id=651 SPR_RS00200 WP_000616166.1 36977..37747(+) (recO) [Streptococcus pneumoniae R6]
MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKRMFFVKHAGQSKLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFP
KINSDLFVMAYATYVAALADASLQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
QAFDFSFKYGTCLCPEHYHEDERRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQELRQFMDQLYEEYVGIHLKSKKF
IDSLADWGQLLKEEKK

Nucleotide

• Length: 771 bp

>NTDB_id=651 SPR_RS00200 WP_000616166.1 36977..37747(+) (recO) [Streptococcus pneumoniae R6]
ATGATTCAGTCTATCACGAGTCAAGGCTTGGTGCTTTACAATCGCAATTTTCGTGAGGATGACAAGCTCGTCAAAATTTT
TACAGAGCAGGTTGGCAAACGCATGTTTTTTGTCAAACACGCTGGTCAGTCTAAGCTGGCGCCTGTTATTCAGCCCTTGG
TGCTGGCACGATTTCTCTTGCGAATCAATGATGACGGACTCAGTTACATCGAAGACTATCATGAGGTCATGACTTTTCCC
AAGATTAATAGTGACCTCTTTGTCATGGCCTATGCGACCTATGTGGCAGCTCTTGCAGATGCTAGTTTGCAGGACAATCA
GCAGGATGCTCCCTTGTTTGCTTTTTTGCAAAAGACTTTGGAGTTGATGGAAGCAGGCTTGGATTATCAGGTTTTGACCA
ATATTTTTGAAATTCAAATTTTGACTCGATTTGGAATCAGCCTCAATTTTAATGAGTGTGTCTTCTGCCATCGGGTTGGT
CAGGCTTTTGACTTTTCTTTCAAATATGGAACCTGTCTCTGTCCAGAGCATTATCATGAGGATGAGAGACGTTGCCATCT
CAATCCCAATATCCCCTATCTGCTCAATCAATTTCAAGCTATTGATTTTGAGACTTTGGAGACCATTTCGCTCAAGCCTG
GAATCAAGCAAGAGCTACGCCAATTTATGGATCAACTATATGAAGAGTACGTTGGGATTCACCTAAAATCAAAGAAATTT
ATTGATTCCCTAGCAGACTGGGGACAATTACTAAAAGAGGAAAAGAAATGA

Domains

Predicted by InterProScan.

• Recombination protein O C terminal (RecO_C) (84-238)

• Recombination protein O N terminal (RecO_N) (4-72)

3D structure

• AlphaFold DB: Q8DRN4

References

• [1]: Vanessa Khemici et al. (2021) Tight Interplay between Replication Stress and Competence Induction in Streptococcus pneumoniae. Cells 10(8):1938. [PMID: 34440707]
• [2]: Calum Johnston et al. (2015) RecFOR is not required for pneumococcal transformation but together with XerS for resolution of chromosome dimers frequently formed in the process. PLoS Genetics 11(1):e1004934. [PMID: 25569614]