Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   LLUC073_RS11775 Genome accession   NZ_CP068698
Coordinates   2270303..2271160 (-) Length   285 a.a.
NCBI ID   WP_318299077.1    Uniprot ID   -
Organism   Lactococcus cremoris strain UC073     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 2263059..2271160 2270303..2271160 within 0
IS/Tn 2271227..2272117 2270303..2271160 flank 67


Gene organization within MGE regions


Location: 2263059..2272117
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LLUC073_RS11725 (LLUC073_11460) - 2264519..2265328 (-) 810 WP_011677177.1 metal ABC transporter permease -
  LLUC073_RS11730 (LLUC073_11465) - 2265321..2266058 (-) 738 WP_011677178.1 metal ABC transporter ATP-binding protein -
  LLUC073_RS11735 (LLUC073_11470) - 2266237..2267079 (-) 843 WP_011677179.1 metal ABC transporter solute-binding protein, Zn/Mn family -
  LLUC073_RS11740 (LLUC073_11475) - 2267076..2267513 (-) 438 WP_282670666.1 zinc-dependent MarR family transcriptional regulator -
  LLUC073_RS11745 (LLUC073_11480) comGG 2267593..2267892 (-) 300 WP_282670667.1 competence type IV pilus minor pilin ComGG Machinery gene
  LLUC073_RS11750 (LLUC073_11485) comGF 2267916..2268341 (-) 426 WP_014735174.1 competence type IV pilus minor pilin ComGF Machinery gene
  LLUC073_RS11755 (LLUC073_11490) comGE 2268325..2268561 (-) 237 WP_014573335.1 competence type IV pilus minor pilin ComGE Machinery gene
  LLUC073_RS11760 (LLUC073_14140) comGD 2268593..2268781 (-) 189 WP_014573336.1 hypothetical protein Machinery gene
  LLUC073_RS11765 (LLUC073_11500) comGC 2268983..2269333 (-) 351 WP_050574401.1 competence type IV pilus major pilin ComGC Machinery gene
  LLUC073_RS11770 (LLUC073_11505) comGB 2269378..2270403 (-) 1026 WP_050574400.1 competence type IV pilus assembly protein ComGB Machinery gene
  LLUC073_RS11775 (LLUC073_11510) comGA 2270303..2271160 (-) 858 WP_318299077.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  LLUC073_RS11780 (LLUC073_11515) - 2271227..2272117 (+) 891 WP_014572485.1 IS982-like element IS982B family transposase -

Sequence

Protein


Download         Length: 285 a.a.        Molecular weight: 32141.73 Da        Isoelectric Point: 6.8466

>NTDB_id=530496 LLUC073_RS11775 WP_318299077.1 2270303..2271160(-) (comGA) [Lactococcus cremoris strain UC073]
MKLTSNSGIGLVLLTHFKFLAGMNTGERRRVQLGACWYELEGSSAKRLRLSTVGDFEGNESLVIRLLHDQKQELEFWFDD
KLQDFRCKRGLYLFAGPVGSGKTSLMFDLAHRHFSNAQVITIEEPVELIESDFIQLQVNDVIGNSYDELIKLSLRHRPDL
LIVGEIRDQQTARAVLRASLTGYTVFSTIHAASVKGVVQRLLELGLSDWKLKNGLEAVVYQRLIAGKGVLDIAKSKFDTW
SPKKWNEKIENLYANGHLTAIEAEREKISINQASKIDTTDGESFE

Nucleotide


Download         Length: 858 bp        

>NTDB_id=530496 LLUC073_RS11775 WP_318299077.1 2270303..2271160(-) (comGA) [Lactococcus cremoris strain UC073]
ATGAAATTAACTAGCAATTCGGGTATTGGTTTAGTATTACTTACTCATTTTAAATTTCTTGCTGGCATGAATACTGGTGA
ACGCCGGCGTGTTCAGCTGGGTGCTTGTTGGTATGAACTAGAGGGAAGTTCGGCAAAACGTTTGCGCCTTTCAACAGTGG
GAGATTTTGAAGGAAATGAATCATTGGTTATCCGTCTTTTGCATGACCAAAAACAAGAACTTGAGTTTTGGTTTGACGAT
AAACTTCAGGATTTTCGATGTAAACGAGGACTTTATTTATTTGCTGGGCCAGTAGGGTCTGGGAAAACTTCACTAATGTT
TGACTTAGCCCACCGTCATTTTTCAAATGCACAGGTTATCACTATTGAGGAGCCTGTAGAATTAATTGAATCTGATTTTA
TTCAGTTACAAGTTAATGATGTAATTGGGAATAGTTATGATGAATTAATTAAACTTTCTTTAAGACACCGACCAGATTTA
CTAATTGTTGGAGAAATTCGAGATCAGCAGACAGCTCGTGCTGTATTGCGTGCAAGTTTAACAGGCTATACAGTTTTTTC
TACTATCCATGCGGCTTCCGTGAAGGGAGTGGTTCAACGTTTGTTGGAATTGGGTTTGAGTGATTGGAAATTGAAAAATG
GATTAGAGGCAGTTGTTTATCAACGGTTAATAGCGGGAAAAGGAGTATTAGATATTGCGAAAAGTAAATTTGACACTTGG
TCGCCGAAAAAATGGAATGAAAAGATTGAGAACTTATATGCAAACGGACATCTTACGGCTATTGAGGCCGAAAGGGAAAA
AATTAGCATTAATCAAGCAAGCAAAATTGATACAACTGATGGGGAATCTTTTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-224)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Lactococcus lactis subsp. cremoris KW2

98.214

98.246

0.965
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

59.073

90.877

0.537
  comGA/cglA/cilD Streptococcus pneumoniae D39

59.073

90.877

0.537
  comGA/cglA/cilD Streptococcus pneumoniae R6

59.073

90.877

0.537
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

59.073

90.877

0.537
  comYA Streptococcus gordonii str. Challis substr. CH1

57.795

92.281

0.533
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

58.915

90.526

0.533
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

58.687

90.877

0.533
  comYA Streptococcus mutans UA159

56.589

90.526

0.512
  comYA Streptococcus mutans UA140

56.589

90.526

0.512
  comGA Latilactobacillus sakei subsp. sakei 23K

42.57

87.368

0.372