Detailed information NTG0530477

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: LLUC073_RS09105
• Genome accession: NZ_CP068698
• Coordinates: 1768682..1769128 (-)
• Length: 148 a.a.
• NCBI ID: WP_318299045.1
• Uniprot ID: -
• Organism: Lactococcus cremoris strain UC073
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
IScluster/Tn	1763519..1768177	1768682..1769128	flank	505

Gene organization within MGE regions

Location: 1763519..1769128

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLUC073_RS09075 (LLUC073_14060)	comEC	1764450..1764959 (-)	510	WP_021165649.1	MBL fold metallo-hydrolase	Machinery gene
LLUC073_RS09080 (LLUC073_14065)	-	1765053..1765424 (-)	372	Protein_1767	ComEC/Rec2 family competence protein	-
LLUC073_RS09085 (LLUC073_08865)	-	1765416..1766591 (+)	1176	WP_120784980.1	IS256-like element IS905 family transposase	-
LLUC073_RS09090 (LLUC073_08870)	comEC	1766638..1767090 (-)	453	WP_405029274.1	ComEC/Rec2 family competence protein	Machinery gene
LLUC073_RS09095 (LLUC073_08875)	-	1767323..1768212 (+)	890	Protein_1770	IS982-like element IS982B family transposase	-
LLUC073_RS09100 (LLUC073_08880)	comEC	1768213..1768554 (-)	342	WP_282671604.1	ComEC/Rec2 family competence protein	Machinery gene
LLUC073_RS09105 (LLUC073_08885)	comEA	1768682..1769128 (-)	447	WP_318299045.1	helix-hairpin-helix domain-containing protein	Machinery gene

Sequence

Protein

• Length: 148 a.a.
• Molecular weight: 16107.12 Da
• Isoelectric Point: 4.8371

>NTDB_id=530477 LLUC073_RS09105 WP_318299045.1 1768682..1769128(-) (comEA) [Lactococcus cremoris strain UC073]
MVDLKGAVAKPNVYQISSDERLVDLIRQAGGFTDQADQKSINLSAKLKDEEVIYVPKLGESSSSESTDSPISSSVSNQVS
TTRGPKININKADLTELQKLTGIGQKKAQDIIDFRMKNGDFKSIEDLGKVSGFGDKTLEKLKDEISID

Nucleotide

• Length: 447 bp

>NTDB_id=530477 LLUC073_RS09105 WP_318299045.1 1768682..1769128(-) (comEA) [Lactococcus cremoris strain UC073]
ATGGTTGATTTGAAAGGAGCGGTTGCAAAACCTAATGTATACCAAATTTCGTCAGATGAGCGCCTTGTTGATTTAATTAG
GCAAGCGGGAGGATTTACTGACCAAGCAGACCAAAAATCAATCAATCTGTCAGCGAAACTTAAAGATGAAGAAGTAATTT
ATGTACCAAAACTTGGGGAAAGTTCAAGTTCAGAAAGTACTGACAGTCCTATTAGCAGCTCTGTCAGTAATCAAGTTTCA
ACGACAAGGGGTCCAAAAATAAATATCAATAAAGCAGACCTGACAGAATTACAAAAACTGACTGGAATTGGTCAAAAAAA
AGCGCAAGATATTATTGATTTTCGCATGAAAAATGGTGACTTTAAATCAATAGAGGATTTGGGTAAAGTATCTGGCTTTG
GGGATAAAACATTAGAAAAATTGAAAGATGAGATTTCTATTGATTAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (1-56)

• Helix-hairpin-helix motif (HHH_3) (85-145)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	95.946	100	0.959
comEA/celA/cilE	Streptococcus mitis SK321	51.02	99.324	0.507
comEA	Latilactobacillus sakei subsp. sakei 23K	44.512	100	0.493
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	48.98	99.324	0.486
comEA/celA/cilE	Streptococcus pneumoniae Rx1	48.299	99.324	0.48
comEA/celA/cilE	Streptococcus pneumoniae D39	48.299	99.324	0.48
comEA/celA/cilE	Streptococcus pneumoniae R6	48.299	99.324	0.48
comEA/celA/cilE	Streptococcus mitis NCTC 12261	47.619	99.324	0.473
comEA	Streptococcus thermophilus LMD-9	43.919	100	0.439
comEA	Staphylococcus aureus N315	41.781	98.649	0.412
comEA	Staphylococcus aureus MW2	41.781	98.649	0.412
comEA	Bacillus subtilis subsp. subtilis str. 168	40.816	99.324	0.405