Detailed information NTG0523213

Bioinformatically predicted

Overview

• Name: pilA2
• Type: Machinery gene
• Locus tag: JH295_RS05470
• Genome accession: NZ_CP066923
• Coordinates: 1297499..1297918 (+)
• Length: 139 a.a.
• NCBI ID: WP_070690005.1
• Uniprot ID: -
• Organism: Xanthomonas campestris pv. incanae strain CFBP1606
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	1295887..1307677	1297499..1297918	within	0

Gene organization within MGE regions

Location: 1295887..1307677

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JH295_RS05465 (JH295_05450)	pilC	1295887..1297146 (-)	1260	WP_070690003.1	type II secretion system F family protein	Machinery gene
JH295_RS05470 (JH295_05455)	pilA2	1297499..1297918 (+)	420	WP_070690005.1	pilin	Machinery gene
JH295_RS05475 (JH295_05460)	pilB	1298161..1299867 (+)	1707	WP_176748120.1	type IV-A pilus assembly ATPase PilB	Machinery gene
JH295_RS05480 (JH295_05465)	-	1300050..1300787 (-)	738	WP_070690009.1	zeta toxin family protein	-
JH295_RS05485 (JH295_05470)	-	1300787..1301149 (-)	363	WP_217694196.1	hypothetical protein	-
JH295_RS05490 (JH295_05475)	pilR	1301341..1302735 (-)	1395	WP_070690014.1	sigma-54 dependent transcriptional regulator	Regulator
JH295_RS05495 (JH295_05480)	-	1302944..1304554 (-)	1611	WP_070690016.1	HAMP domain-containing sensor histidine kinase	-
JH295_RS05500 (JH295_05485)	sucC	1304788..1305957 (+)	1170	WP_011038209.1	ADP-forming succinate--CoA ligase subunit beta	-
JH295_RS05505 (JH295_05490)	sucD	1305982..1306857 (+)	876	WP_011038208.1	succinate--CoA ligase subunit alpha	-
JH295_RS05510 (JH295_05495)	-	1306961..1307371 (+)	411	WP_029628876.1	DNA-binding protein	-
JH295_RS05515 (JH295_05500)	-	1307375..1307677 (+)	303	WP_011038207.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Sequence

Protein

• Length: 139 a.a.
• Molecular weight: 14028.18 Da
• Isoelectric Point: 9.0169

>NTDB_id=523213 JH295_RS05470 WP_070690005.1 1297499..1297918(+) (pilA2) [Xanthomonas campestris pv. incanae strain CFBP1606]
MKKQQGFTLIELMIVVAIIAILAAIALPAYQDYLIKARVSEVVLAASSARTTVSEAAAADTGTAMPAAVTIESQASKYVA
SVAYAGGTTNGIITATSKDLGGTATGTITLTGTKSTNGQVAWVCGGTIPAKYRPATCQG

Nucleotide

• Length: 420 bp

>NTDB_id=523213 JH295_RS05470 WP_070690005.1 1297499..1297918(+) (pilA2) [Xanthomonas campestris pv. incanae strain CFBP1606]
ATGAAGAAGCAACAAGGTTTTACCCTGATTGAATTGATGATCGTCGTGGCCATCATTGCCATTCTTGCCGCTATCGCGCT
GCCGGCCTATCAGGATTACCTGATCAAGGCTCGCGTATCCGAGGTGGTTCTGGCTGCATCTTCCGCGCGTACTACAGTTT
CTGAAGCCGCGGCGGCTGACACTGGTACGGCCATGCCTGCCGCAGTTACGATTGAGAGCCAGGCGTCCAAGTACGTAGCA
TCTGTTGCTTATGCCGGCGGCACCACAAATGGTATTATCACTGCAACCTCCAAGGATCTTGGCGGTACTGCTACTGGAAC
GATTACGTTGACCGGTACCAAGTCTACTAATGGACAGGTCGCCTGGGTCTGCGGCGGCACGATCCCGGCTAAGTATCGTC
CGGCAACTTGCCAGGGCTAA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (1-27)

• Pilin (bacterial filament) (Pilin) (36-138)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA2	Legionella pneumophila str. Paris	53.901	100	0.547
pilA2	Legionella pneumophila strain ERS1305867	53.191	100	0.54
comP	Acinetobacter baylyi ADP1	47.651	100	0.511
pilE	Neisseria elongata subsp. glycolytica ATCC 29315	37.5	100	0.496
pilA	Ralstonia pseudosolanacearum GMI1000	41.104	100	0.482
pilA/pilA1	Eikenella corrodens VA1	39.474	100	0.432
pilE	Neisseria gonorrhoeae MS11	36.875	100	0.424
pilE	Neisseria gonorrhoeae strain FA1090	35.404	100	0.41
pilA	Vibrio parahaemolyticus RIMD 2210633	39.855	99.281	0.396
pilA	Acinetobacter baumannii strain A118	38.571	100	0.388
pilA/pilAI	Pseudomonas stutzeri DSM 10701	37.324	100	0.381