Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   JDW18_RS22765 Genome accession   NZ_CP066783
Coordinates   4807308..4807754 (-) Length   148 a.a.
NCBI ID   WP_218241755.1    Uniprot ID   -
Organism   Comamonas fluminis strain CJ34     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4802308..4812754
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JDW18_RS22260 - 4802538..4802987 (+) 450 WP_218241750.1 hypothetical protein -
  JDW18_RS22265 - 4803105..4803455 (+) 351 WP_218241751.1 phage holin family protein -
  JDW18_RS22270 - 4803414..4804964 (-) 1551 WP_246610182.1 M48 family metalloprotease -
  JDW18_RS22275 moaC 4805258..4805728 (+) 471 WP_218241753.1 cyclic pyranopterin monophosphate synthase MoaC -
  JDW18_RS22280 - 4805635..4807173 (-) 1539 WP_218241754.1 O-antigen ligase family protein -
  JDW18_RS22765 pilE 4807308..4807754 (-) 447 WP_218241755.1 pilin Machinery gene
  JDW18_RS22290 - 4807949..4808662 (-) 714 WP_218241756.1 FHA domain-containing protein -
  JDW18_RS22295 ssb 4808959..4809507 (-) 549 WP_218241757.1 single-stranded DNA-binding protein Machinery gene
  JDW18_RS22300 - 4809624..4810799 (-) 1176 WP_218244031.1 MFS transporter -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 15013.13 Da        Isoelectric Point: 8.9423

>NTDB_id=522523 JDW18_RS22765 WP_218241755.1 4807308..4807754(-) (pilE) [Comamonas fluminis strain CJ34]
MKRSIQKGFTLIELMIVVAIIGILAAVALPAYQDYTTRAKVSEMVLAASAGRTSLSEAYAAKSAFPAAASAADYITNASS
KYVASVAYQDSNSGAQAQITVTGTSALGGTANGTTLTLTGVGDTDTGTVKWTCAAGTMPAKYLPASCK

Nucleotide


Download         Length: 447 bp        

>NTDB_id=522523 JDW18_RS22765 WP_218241755.1 4807308..4807754(-) (pilE) [Comamonas fluminis strain CJ34]
ATGAAGCGTTCCATTCAAAAGGGTTTTACCCTGATCGAACTGATGATCGTTGTGGCGATCATCGGTATTCTGGCTGCCGT
GGCTCTGCCTGCTTATCAGGATTACACCACACGTGCTAAGGTTTCTGAAATGGTTCTGGCTGCGTCTGCTGGTCGTACTT
CGTTGTCTGAAGCTTATGCTGCCAAGAGTGCATTCCCTGCAGCTGCATCTGCTGCCGATTACATTACCAATGCTTCCTCG
AAGTATGTTGCCAGCGTTGCTTACCAAGACTCGAATTCTGGTGCACAAGCGCAGATCACTGTGACTGGTACTAGTGCTTT
AGGTGGTACTGCAAATGGAACAACTTTGACTTTGACTGGTGTGGGGGACACTGATACTGGCACAGTTAAGTGGACATGTG
CTGCGGGAACTATGCCTGCAAAGTATCTGCCTGCTAGCTGCAAGTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(4-29)

Pilin (bacterial filament) (Pilin)

(37-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

40.957

100

0.52
  pilA2 Legionella pneumophila str. Paris

51.049

96.622

0.493
  pilA2 Legionella pneumophila strain ERS1305867

50.35

96.622

0.486
  pilA Ralstonia pseudosolanacearum GMI1000

41.52

100

0.48
  pilA/pilA1 Eikenella corrodens VA1

40.625

100

0.439
  pilE Neisseria gonorrhoeae MS11

38.323

100

0.432
  comP Acinetobacter baylyi ADP1

42.282

100

0.426
  pilE Neisseria gonorrhoeae strain FA1090

38.608

100

0.412
  pilA/pilAI Pseudomonas stutzeri DSM 10701

39.456

99.324

0.392
  pilA Acinetobacter nosocomialis M2

38.732

95.946

0.372