Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   JEZ96_RS13450 Genome accession   NZ_CP066370
Coordinates   3012029..3013141 (-) Length   370 a.a.
NCBI ID   WP_011788675.1    Uniprot ID   A0A6N3J369
Organism   Shewanella putrefaciens strain ATCC 8071     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3007029..3018141
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JEZ96_RS13415 - 3007562..3008122 (-) 561 WP_011788682.1 DUF4136 domain-containing protein -
  JEZ96_RS13420 - 3008301..3008615 (+) 315 WP_011919670.1 hypothetical protein -
  JEZ96_RS13425 yciH 3008781..3009110 (+) 330 WP_014611015.1 stress response translation initiation inhibitor YciH -
  JEZ96_RS13430 - 3009223..3009786 (+) 564 WP_011788679.1 YqgE/AlgH family protein -
  JEZ96_RS13435 ruvX 3009818..3010243 (+) 426 WP_025008700.1 Holliday junction resolvase RuvX -
  JEZ96_RS13440 hemH 3010305..3011318 (-) 1014 WP_011788677.1 ferrochelatase -
  JEZ96_RS13445 - 3011351..3011890 (-) 540 WP_025008699.1 glutathione peroxidase -
  JEZ96_RS13450 pilU 3012029..3013141 (-) 1113 WP_011788675.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  JEZ96_RS13455 pilT 3013151..3014188 (-) 1038 WP_011788674.1 type IV pilus twitching motility protein PilT Machinery gene
  JEZ96_RS13460 - 3014224..3014922 (+) 699 WP_128090277.1 YggS family pyridoxal phosphate-dependent enzyme -
  JEZ96_RS13465 proC 3015103..3015921 (+) 819 WP_014611018.1 pyrroline-5-carboxylate reductase -
  JEZ96_RS13470 - 3015968..3016516 (+) 549 WP_061783209.1 YggT family protein -
  JEZ96_RS13475 yggU 3016516..3016806 (+) 291 WP_011919678.1 DUF167 family protein YggU -
  JEZ96_RS13480 - 3016884..3017318 (+) 435 WP_025008695.1 DUF4426 domain-containing protein -
  JEZ96_RS13485 rdgB 3017429..3018046 (+) 618 WP_025008694.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41097.28 Da        Isoelectric Point: 6.3723

>NTDB_id=519507 JEZ96_RS13450 WP_011788675.1 3012029..3013141(-) (pilU) [Shewanella putrefaciens strain ATCC 8071]
MDVRPFLKVIVERKASDLFITAGFPPSAKIDGELRPLAESAFTPAQSLDFVESLMTEAQKQEFHSTRECNFAFAAKDLGR
FRVSAFWQRESPGCVMRRIETKIPEVDDLKLPPILKDLVMSKRGLIIMVGGTGTGKSTSLAALVGYRNAHARGHILTIED
PVEFVHDHRKSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHQQLLFDLSLNLRGIVAQQLIPKVDGTGRRAAIEVLINTPRVASLIAKNELHLLKETMAKSNEQGMQTFDQA
LLKLYVDGEISYADALHHADSPNDLRLMIKLQNKEPTGSSFMEGVTLDMD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=519507 JEZ96_RS13450 WP_011788675.1 3012029..3013141(-) (pilU) [Shewanella putrefaciens strain ATCC 8071]
ATGGATGTACGTCCGTTTTTAAAAGTCATCGTTGAGCGTAAAGCCTCTGATTTGTTTATTACCGCGGGCTTTCCTCCCAG
TGCAAAAATTGATGGCGAATTACGTCCCCTCGCCGAGAGTGCATTTACGCCTGCACAGTCTTTAGATTTTGTTGAGTCAC
TCATGACTGAAGCGCAGAAACAGGAATTTCACTCAACCCGTGAATGTAACTTTGCTTTTGCGGCAAAGGATCTCGGTCGT
TTCCGAGTCAGCGCTTTTTGGCAAAGGGAATCACCAGGATGTGTTATGCGCCGGATTGAGACCAAAATTCCCGAAGTCGA
TGACTTAAAATTACCTCCTATTTTAAAAGATCTGGTGATGAGCAAGCGTGGGCTTATCATTATGGTCGGCGGCACAGGTA
CGGGTAAGTCAACCTCATTAGCAGCATTGGTTGGATACCGTAATGCCCATGCCCGTGGCCATATATTGACGATTGAAGAT
CCTGTTGAATTTGTTCACGATCATAGAAAAAGCATTATCACCCAACGTGAAGTAGGCATAGATACGGAGTCATTCGATGC
AGCATTAAAAAGCTCCCTTCGTCAGGCGCCCGATGTGATCTTAATCGGTGAAATTCGTACCCAAGAGACGATGGAATTTG
CATTGTCGTTTGCAGAAACAGGTCATCTTTGTATGGCAACCCTACATGCCAATAATGCGAACCAAGCACTCGATCGGATT
ATGCACTTAGTGCCTGAGAGTAAACATCAGCAGTTACTATTTGATTTATCCTTAAATTTACGTGGTATTGTGGCGCAGCA
ATTGATCCCCAAAGTCGATGGAACGGGGCGCCGCGCCGCGATTGAAGTGTTAATTAATACTCCAAGGGTGGCGAGTTTAA
TTGCCAAAAATGAACTGCATTTACTCAAGGAAACCATGGCAAAATCGAATGAGCAGGGGATGCAGACATTCGACCAAGCG
TTACTGAAATTATATGTTGATGGTGAAATTAGTTATGCCGATGCCTTACACCATGCTGACTCACCAAACGATTTACGTTT
GATGATCAAATTACAAAATAAAGAGCCTACTGGTTCAAGTTTTATGGAGGGTGTAACACTCGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6N3J369

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.215

100

0.605
  pilU Vibrio cholerae strain A1552

58.649

100

0.586
  pilU Acinetobacter baylyi ADP1

61.08

95.135

0.581
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.87

93.243

0.381
  pilT Vibrio cholerae strain A1552

42.727

89.189

0.381
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.727

89.189

0.381
  pilT Acinetobacter baumannii D1279779

39.181

92.432

0.362
  pilT Acinetobacter baumannii strain A118

39.181

92.432

0.362
  pilT Acinetobacter nosocomialis M2

39.181

92.432

0.362
  pilT Legionella pneumophila strain Lp02

40.483

89.459

0.362
  pilT Legionella pneumophila strain ERS1305867

40.483

89.459

0.362