Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   JET17_RS01935 Genome accession   NZ_CP066306
Coordinates   406014..407282 (+) Length   422 a.a.
NCBI ID   WP_012312329.1    Uniprot ID   -
Organism   Pseudomonas putida strain BR-PH17     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 401014..412282
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JET17_RS01920 (JET17_01920) pilM 403693..404541 (+) 849 WP_012312326.1 type IV pilus biogenesis protein PilM -
  JET17_RS01925 (JET17_01925) - 404541..405077 (+) 537 WP_012312327.1 PilN domain-containing protein -
  JET17_RS01930 (JET17_01930) - 405074..406090 (+) 1017 WP_012312328.1 pilus assembly protein PilP -
  JET17_RS01935 (JET17_01935) pilQ 406014..407282 (+) 1269 WP_012312329.1 type IV pilus secretin PilQ Machinery gene
  JET17_RS01940 (JET17_01940) aroK 407287..407805 (+) 519 WP_039602073.1 shikimate kinase AroK -
  JET17_RS01945 (JET17_01945) aroB 407900..408997 (+) 1098 WP_012312331.1 3-dehydroquinate synthase -
  JET17_RS01950 (JET17_01950) - 409013..410593 (+) 1581 WP_012312332.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 422 a.a.        Molecular weight: 46017.86 Da        Isoelectric Point: 4.6998

>NTDB_id=519183 JET17_RS01935 WP_012312329.1 406014..407282(+) (pilQ) [Pseudomonas putida strain BR-PH17]
MNGLMKWLVAALLPMPLLAATPYRGEPISLNFQDVEVRLVLQVLADYAGINLVASDTVQGSITLRLQDVPWDQALELVLR
SKGLGRREEGNVLLVAPAVELAEQALGARMGLELEAQSRPLRRELLPIHHAEASELAELLLATLADDGILTGRGSLSVDV
RTNTLVAHQPEDRLAELRQLVAQLDVPVRQVEIEARIVEANVDYENSLGVRWGPRLYSEAPALGKDVFVDLGMERAGSSI
GFGVLRGGVLLDLELSAMEKTGNGEIISQPKVVTANNETARILKGTEVPYQETTKSGATSVTFREASLSLEVTPQITPDG
KVIMTVKVTKDEPDFVNALNDVPPIRKNEVNAKVRVADGETIVIGGVYSTTQNNVVDKVPFFGDLPYVGRLFRRDALQEK
KSELLVFLTPRIMNDQAIAVSR

Nucleotide


Download         Length: 1269 bp        

>NTDB_id=519183 JET17_RS01935 WP_012312329.1 406014..407282(+) (pilQ) [Pseudomonas putida strain BR-PH17]
ATGAACGGGTTGATGAAGTGGCTGGTGGCGGCATTGCTGCCGATGCCGTTGCTGGCGGCAACGCCTTATCGGGGTGAACC
GATTTCGCTGAACTTCCAGGACGTAGAAGTGCGCCTTGTGTTGCAGGTGCTTGCCGATTATGCCGGGATCAATCTGGTCG
CAAGCGACACGGTGCAGGGCAGTATTACCTTGCGTCTGCAGGATGTTCCTTGGGATCAGGCTCTGGAACTGGTGTTGCGC
AGCAAAGGGCTGGGCCGGCGGGAGGAGGGCAATGTGCTGTTGGTGGCGCCAGCGGTTGAGCTGGCCGAACAGGCTCTGGG
AGCGCGCATGGGGTTGGAGCTGGAGGCCCAATCGCGGCCGTTGCGCCGCGAGTTGCTGCCGATACACCACGCCGAGGCCT
CGGAACTTGCCGAGTTGCTGCTGGCTACGTTGGCCGATGACGGCATTCTGACTGGCCGCGGTAGTTTGAGTGTGGATGTG
CGCACCAATACGCTGGTTGCGCACCAGCCTGAGGACCGGCTTGCCGAGTTGCGCCAGTTGGTTGCGCAACTGGATGTGCC
GGTACGACAGGTAGAGATCGAGGCGCGCATCGTGGAGGCCAATGTCGATTACGAGAACAGCCTTGGGGTGCGCTGGGGCC
CTCGCCTCTACAGCGAAGCCCCTGCTTTGGGCAAGGACGTGTTTGTTGATCTTGGCATGGAGCGGGCCGGCTCCAGCATC
GGCTTTGGAGTGTTGCGTGGGGGCGTGCTGTTGGACCTTGAACTCAGCGCCATGGAGAAGACCGGCAATGGTGAAATCAT
TTCCCAGCCCAAGGTGGTCACCGCTAACAACGAAACTGCCCGCATCCTCAAAGGTACCGAAGTCCCTTACCAGGAGACCA
CGAAGAGTGGCGCTACGTCGGTTACATTCCGCGAGGCGTCGCTGTCGTTGGAGGTCACTCCGCAGATCACGCCGGACGGC
AAGGTGATCATGACGGTCAAGGTGACCAAGGACGAGCCTGATTTCGTCAATGCCTTGAACGATGTGCCGCCGATCCGCAA
GAACGAGGTCAATGCCAAGGTACGGGTAGCTGACGGGGAAACCATCGTCATTGGCGGTGTGTATTCGACTACGCAAAACA
ATGTGGTGGACAAGGTGCCATTTTTCGGCGATCTGCCGTATGTTGGGCGGCTGTTTCGACGCGATGCATTACAAGAGAAA
AAATCCGAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAATGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(257-413)

Bacterial type II/III secretion system short domain (Secretin_N)

(125-190)

Secretin and TonB N terminus short domain (STN)

(51-97)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

58.503

100

0.611
  comQ Acinetobacter baylyi ADP1

41.242

100

0.441
  pilQ Acinetobacter baumannii strain A118

39.871

100

0.438
  pilQ Acinetobacter baumannii D1279779

39.871

100

0.438
  pilQ Legionella pneumophila strain Lp02

43.23

99.763

0.431
  pilQ Legionella pneumophila strain ERS1305867

43.23

99.763

0.431
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

42.243

99.289

0.419
  pilQ Vibrio cholerae strain A1552

42.243

99.289

0.419
  pilQ Vibrio campbellii strain DS40M4

40.898

100

0.41
  pilQ Neisseria gonorrhoeae strain FA1090

36.643

100

0.367
  pilQ Neisseria meningitidis MC58

36.019

100

0.36
  pilQ Neisseria meningitidis M1080

36.019

100

0.36