Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ITG10_RS06380 Genome accession   NZ_CP066149
Coordinates   1417966..1419684 (-) Length   572 a.a.
NCBI ID   WP_128644404.1    Uniprot ID   -
Organism   Vibrio sp. ED004     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1412966..1424684
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ITG10_RS06360 (ITG10_06290) - 1413645..1414490 (-) 846 WP_017632098.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  ITG10_RS06365 (ITG10_06295) - 1414560..1416077 (-) 1518 WP_017632097.1 AAA family ATPase -
  ITG10_RS06370 (ITG10_06300) aroB 1416157..1417245 (-) 1089 WP_017061124.1 3-dehydroquinate synthase -
  ITG10_RS06375 (ITG10_06305) aroK 1417271..1417789 (-) 519 WP_009848907.1 shikimate kinase AroK -
  ITG10_RS06380 (ITG10_06310) pilQ 1417966..1419684 (-) 1719 WP_128644404.1 type IV pilus secretin PilQ family protein Machinery gene
  ITG10_RS06385 (ITG10_06315) pilP 1419720..1420238 (-) 519 WP_017066198.1 pilus assembly protein PilP Machinery gene
  ITG10_RS06390 (ITG10_06320) pilO 1420225..1420836 (-) 612 WP_017632095.1 type 4a pilus biogenesis protein PilO Machinery gene
  ITG10_RS06395 (ITG10_06325) pilN 1420829..1421428 (-) 600 WP_017632094.1 PilN domain-containing protein Machinery gene
  ITG10_RS06400 (ITG10_06330) pilM 1421412..1422437 (-) 1026 WP_017632093.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 62150.78 Da        Isoelectric Point: 4.3615

>NTDB_id=517873 ITG10_RS06380 WP_128644404.1 1417966..1419684(-) (pilQ) [Vibrio sp. ED004]
MNKGISGLVSKALQGFAVSVMLVFSVLSHAESLPNKLENIDFRVNKDKDAVIIIELATSTAVVDVQRAQEGLSIELLNTQ
VDDDKLYLLDVKDFATLVEGIEVYKETPSTRLLATINNDYQYEYNLKGRFIEVVISKPVIDETTAEKSILEKEGKLISIN
FQDIPVRNVLQLIADYNDFNLVVSDSVAGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKAELDLREQQALEK
SRLEEELGELKSEIIKINFAKATDIADMIGGEGAVSMLSDRGSITIDERTNSLLIRELEENIAVIRGIIESLDIPVKQVQ
IEARIVTVTEGNLDELGVRWGVSSTNGSFTVGGSIEGNHPSQITPYDDDGGNSVIDDYLNVNLGATSPNASSIAFQVAKL
GSDTLLDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATSVAFKKAVLSLKVTPQITPDNRLVLD
LSVTQDRPGQVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSVSSTVDKVPVLGDLPLLGALFRRSYENVGKSE
LLIFVTPKVVIQ

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=517873 ITG10_RS06380 WP_128644404.1 1417966..1419684(-) (pilQ) [Vibrio sp. ED004]
ATGAATAAAGGAATAAGTGGATTAGTGAGCAAGGCTCTACAGGGTTTTGCTGTGTCTGTGATGTTGGTTTTTAGCGTGCT
CAGCCACGCAGAGAGCTTGCCTAATAAATTAGAGAATATTGATTTTAGGGTTAATAAGGATAAAGACGCCGTCATCATTA
TTGAACTGGCCACCAGTACCGCGGTGGTCGATGTTCAGCGTGCTCAGGAAGGCTTGAGTATAGAGTTACTTAATACTCAG
GTTGATGACGATAAACTCTACCTTCTGGATGTGAAAGATTTCGCTACCTTAGTTGAAGGGATTGAAGTCTACAAAGAGAC
ACCGAGCACTCGGCTTCTCGCGACCATCAATAATGACTACCAGTATGAATACAACTTAAAAGGTCGCTTCATTGAGGTAG
TGATCAGCAAGCCGGTTATCGATGAAACGACAGCTGAGAAAAGCATCCTAGAGAAAGAGGGCAAGCTGATCTCAATTAAC
TTCCAAGATATCCCAGTACGAAACGTCCTTCAGTTGATCGCTGACTATAACGACTTCAACCTTGTCGTGTCTGATTCGGT
CGCGGGTAACCTGACTTTACGTTTAGATGGCGTACCTTGGCAGCAAGTTCTCGACATTATCTTGCAAGTTAAAGGGTTGG
ATAAGCGCGTGGATGGCAATGTCATCTTGGTTGCACCGAAAGCTGAGTTGGATCTTCGAGAGCAGCAAGCGCTCGAGAAA
TCTCGTTTGGAAGAGGAACTAGGAGAGCTCAAGTCGGAAATCATCAAGATCAACTTTGCTAAAGCCACCGACATCGCTGA
CATGATTGGTGGTGAGGGTGCGGTGAGTATGTTGTCTGATCGCGGCTCTATTACCATTGATGAGCGCACTAACTCTTTGT
TGATTCGTGAGTTGGAAGAGAACATTGCCGTGATACGAGGCATCATTGAATCCTTGGATATCCCAGTAAAGCAGGTGCAA
ATAGAGGCGCGTATCGTGACTGTCACTGAGGGTAATCTTGATGAGCTTGGCGTGCGCTGGGGTGTATCCTCGACCAACGG
AAGCTTCACCGTTGGTGGTTCAATAGAAGGTAATCATCCATCACAAATAACACCTTATGACGATGATGGCGGCAACAGTG
TGATTGATGACTACCTCAACGTTAACTTGGGTGCGACATCGCCGAATGCATCGAGCATTGCCTTTCAGGTGGCCAAGCTT
GGCTCGGATACGTTGCTCGACCTTGAACTGTCGGCACTGCAACAAGAGTCGAAAGCTGAGATCATTTCTAGCCCACGCTT
AATCACCACCAATAAAAAGCCCGCTTATATTGAGCAAGGTACTGAAATTCCTTACTTAGAGTCCTCTTCAAGTGGTGCTA
CATCGGTCGCATTTAAGAAAGCGGTATTGAGTCTGAAAGTGACGCCGCAGATAACGCCCGACAATCGCTTGGTGTTGGAT
TTGAGTGTGACTCAAGATAGGCCCGGCCAAGTGGTAAAAACCGGAACTGGGGAAGCGGTCGCGATAGATACCCAAAGAAT
AGGTACGCAAGTGCTTGTTAATAATGGTGAAACGGTCGTTCTTGGTGGAATATTTCAACACAGCGTGAGCAGCACAGTCG
ATAAAGTTCCTGTGTTAGGAGACCTACCTTTGTTAGGTGCATTGTTCCGTCGCAGCTATGAAAATGTGGGCAAAAGTGAA
CTGCTTATTTTTGTCACGCCTAAAGTTGTGATTCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(412-570)

Bacterial type II/III secretion system short domain (Secretin_N)

(253-318)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae strain A1552

77.003

100

0.773
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

77.003

100

0.773
  pilQ Vibrio campbellii strain DS40M4

80.699

95.105

0.767
  pilQ Legionella pneumophila strain ERS1305867

42.491

95.455

0.406
  pilQ Legionella pneumophila strain Lp02

42.491

95.455

0.406
  pilQ Pseudomonas aeruginosa PAK

40.754

97.378

0.397
  comQ Acinetobacter baylyi ADP1

38.515

100

0.39
  pilQ Acinetobacter baumannii D1279779

37.478

100

0.379
  pilQ Acinetobacter baumannii strain A118

37.478

100

0.379