Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   ITG10_RS06395 Genome accession   NZ_CP066149
Coordinates   1420829..1421428 (-) Length   199 a.a.
NCBI ID   WP_017632094.1    Uniprot ID   -
Organism   Vibrio sp. ED004     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1415829..1426428
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ITG10_RS06370 (ITG10_06300) aroB 1416157..1417245 (-) 1089 WP_017061124.1 3-dehydroquinate synthase -
  ITG10_RS06375 (ITG10_06305) aroK 1417271..1417789 (-) 519 WP_009848907.1 shikimate kinase AroK -
  ITG10_RS06380 (ITG10_06310) pilQ 1417966..1419684 (-) 1719 WP_128644404.1 type IV pilus secretin PilQ family protein Machinery gene
  ITG10_RS06385 (ITG10_06315) pilP 1419720..1420238 (-) 519 WP_017066198.1 pilus assembly protein PilP Machinery gene
  ITG10_RS06390 (ITG10_06320) pilO 1420225..1420836 (-) 612 WP_017632095.1 type 4a pilus biogenesis protein PilO Machinery gene
  ITG10_RS06395 (ITG10_06325) pilN 1420829..1421428 (-) 600 WP_017632094.1 PilN domain-containing protein Machinery gene
  ITG10_RS06400 (ITG10_06330) pilM 1421412..1422437 (-) 1026 WP_017632093.1 type IV pilus assembly protein PilM Machinery gene
  ITG10_RS06405 (ITG10_06335) - 1422589..1425150 (+) 2562 WP_164913310.1 PBP1A family penicillin-binding protein -
  ITG10_RS06410 (ITG10_06340) oxyR 1425262..1426155 (-) 894 WP_017632091.1 DNA-binding transcriptional regulator OxyR -
  ITG10_RS06415 - 1426154..1426333 (+) 180 WP_128644403.1 hypothetical protein -

Sequence

Protein


Download         Length: 199 a.a.        Molecular weight: 22870.19 Da        Isoelectric Point: 8.5093

>NTDB_id=517876 ITG10_RS06395 WP_017632094.1 1420829..1421428(-) (pilN) [Vibrio sp. ED004]
MLHQINLLPWRDEIRAQHKKRFVHLVILGVIIALGGQWAVGNYFHDQQAKQQARLNYLNQYIAELDRQIQSLKVAEQEHK
AILTRLDVVESLQLGRNKTTDFMNLMPELIPEGVYVDKIKMNGQEIEMSGISDSTARLATMLDNLERSDSLEGVEMHSIV
HNRKRFNKEFQTFKVSFVFSPVSLESTTANRKGKEANHG

Nucleotide


Download         Length: 600 bp        

>NTDB_id=517876 ITG10_RS06395 WP_017632094.1 1420829..1421428(-) (pilN) [Vibrio sp. ED004]
ATGTTGCATCAAATTAACCTGCTGCCTTGGCGCGATGAGATTCGTGCTCAACATAAGAAGCGTTTCGTTCATTTGGTTAT
TCTTGGCGTCATCATTGCGCTTGGTGGGCAATGGGCGGTCGGTAACTATTTTCACGACCAACAAGCCAAGCAGCAAGCGC
GCCTCAACTATCTCAATCAATACATCGCTGAGCTCGACCGACAGATTCAGTCATTAAAAGTCGCCGAGCAAGAGCACAAA
GCCATTCTCACTCGACTGGATGTGGTGGAGTCTCTGCAGCTTGGCCGCAATAAGACCACCGACTTCATGAACCTGATGCC
GGAGCTGATTCCTGAAGGCGTGTATGTCGACAAGATAAAGATGAATGGTCAGGAGATTGAAATGTCGGGCATCAGCGACA
GTACGGCTCGTCTCGCCACTATGTTGGACAACCTAGAACGATCTGATTCTCTGGAAGGGGTTGAGATGCACTCTATCGTT
CATAACCGCAAGCGCTTCAATAAAGAGTTCCAGACCTTCAAGGTCTCTTTTGTGTTCAGTCCCGTTTCTTTAGAAAGCAC
CACGGCGAACCGTAAAGGGAAGGAGGCGAATCATGGCTAA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(103-174)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio cholerae strain A1552

65.761

92.462

0.608
  pilN Vibrio campbellii strain DS40M4

63.441

93.467

0.593