Detailed information NTG0517877

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: ITG10_RS06400
• Genome accession: NZ_CP066149
• Coordinates: 1421412..1422437 (-)
• Length: 341 a.a.
• NCBI ID: WP_017632093.1
• Uniprot ID: -
• Organism: Vibrio sp. ED004
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1416412..1427437

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ITG10_RS06375 (ITG10_06305)	aroK	1417271..1417789 (-)	519	WP_009848907.1	shikimate kinase AroK	-
ITG10_RS06380 (ITG10_06310)	pilQ	1417966..1419684 (-)	1719	WP_128644404.1	type IV pilus secretin PilQ family protein	Machinery gene
ITG10_RS06385 (ITG10_06315)	pilP	1419720..1420238 (-)	519	WP_017066198.1	pilus assembly protein PilP	Machinery gene
ITG10_RS06390 (ITG10_06320)	pilO	1420225..1420836 (-)	612	WP_017632095.1	type 4a pilus biogenesis protein PilO	Machinery gene
ITG10_RS06395 (ITG10_06325)	pilN	1420829..1421428 (-)	600	WP_017632094.1	PilN domain-containing protein	Machinery gene
ITG10_RS06400 (ITG10_06330)	pilM	1421412..1422437 (-)	1026	WP_017632093.1	type IV pilus assembly protein PilM	Machinery gene
ITG10_RS06405 (ITG10_06335)	-	1422589..1425150 (+)	2562	WP_164913310.1	PBP1A family penicillin-binding protein	-
ITG10_RS06410 (ITG10_06340)	oxyR	1425262..1426155 (-)	894	WP_017632091.1	DNA-binding transcriptional regulator OxyR	-
ITG10_RS06415	-	1426154..1426333 (+)	180	WP_128644403.1	hypothetical protein	-
ITG10_RS06420 (ITG10_06345)	-	1426378..1427106 (+)	729	WP_017632090.1	glutathione peroxidase	-

Sequence

Protein

• Length: 341 a.a.
• Molecular weight: 38209.76 Da
• Isoelectric Point: 6.7737

>NTDB_id=517877 ITG10_RS06400 WP_017632093.1 1421412..1422437(-) (pilM) [Vibrio sp. ED004]
MGSSLITGIDINHHSIKAVVLKPVGELYALVGYKELPISDDIFTANHTLEYQKTVKKLKELRKGLPFGCRNVAISVPDNT
VISKVLQIESELEDREKEFSIYQTFAHQSPFPIEELSLDFVKLEDKRFGKGSTSSYQVYATRKEVVDSRADTLTKAGFKP
VVVDTQAHGLLNIWQLASRLYPDKKNWLLVDVGIDQTSLGVIPLSSAPFYKDIAFGTQDLRSSDNPSDIEGVFGTAEDTH
RFIVNLIEKLKRQLQLYSSVNALQPISGIWLMGEGASIPMVTEELERHFQLSCESLNPLSLFENKVAKRRRLPMDWQHFG
IAAGMAMSGLKWQGEKHVASN

Nucleotide

• Length: 1026 bp

>NTDB_id=517877 ITG10_RS06400 WP_017632093.1 1421412..1422437(-) (pilM) [Vibrio sp. ED004]
ATGGGTTCATCATTAATTACAGGTATAGATATAAATCATCACAGCATCAAAGCCGTGGTACTAAAACCCGTGGGGGAGTT
GTATGCCCTAGTGGGATACAAAGAGCTGCCGATTTCGGACGACATTTTTACAGCTAACCATACTTTGGAGTATCAGAAAA
CTGTTAAGAAACTCAAAGAACTTAGGAAAGGACTGCCTTTTGGCTGTCGCAACGTCGCCATTTCGGTACCTGATAACACA
GTGATCAGCAAAGTACTGCAAATAGAGAGTGAGTTAGAAGACAGAGAAAAAGAGTTTTCGATCTATCAAACCTTCGCTCA
CCAGTCTCCCTTTCCTATCGAGGAGTTGAGTTTAGATTTTGTAAAGCTGGAAGACAAACGATTTGGCAAAGGATCGACAA
GCAGTTATCAGGTGTATGCCACTCGCAAGGAAGTGGTTGATAGCCGAGCAGATACGCTCACTAAAGCAGGTTTTAAACCT
GTGGTCGTGGATACGCAGGCGCATGGGCTACTCAATATCTGGCAATTGGCATCACGCTTGTATCCCGATAAGAAGAACTG
GTTGCTGGTGGATGTTGGAATCGACCAAACCTCGCTAGGGGTTATCCCGCTGAGTTCAGCACCTTTCTATAAAGACATCG
CCTTTGGTACGCAAGATCTTCGCAGCTCGGATAACCCGAGTGATATAGAAGGCGTATTCGGCACGGCTGAAGATACCCAC
CGATTTATTGTCAATTTGATCGAAAAGCTCAAGCGTCAGTTGCAGCTCTATTCTTCAGTCAATGCACTTCAGCCTATTTC
AGGGATCTGGCTAATGGGCGAGGGCGCGAGCATCCCGATGGTCACTGAAGAGCTTGAGCGCCATTTTCAGCTGAGCTGTG
AGTCGCTTAATCCTTTATCTCTGTTTGAGAACAAAGTAGCGAAGCGACGTCGACTGCCCATGGACTGGCAACACTTTGGC
ATTGCCGCGGGTATGGCGATGAGTGGGCTCAAGTGGCAGGGAGAAAAGCATGTTGCATCAAATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (8-220)

• Type IV pilus assembly protein PilM; (PilM_2) (242-328)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Vibrio cholerae strain A1552	54.839	100	0.548
pilM	Vibrio campbellii strain DS40M4	47.661	100	0.478