Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   ITG10_RS06390 Genome accession   NZ_CP066149
Coordinates   1420225..1420836 (-) Length   203 a.a.
NCBI ID   WP_017632095.1    Uniprot ID   -
Organism   Vibrio sp. ED004     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1415225..1425836
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ITG10_RS06370 (ITG10_06300) aroB 1416157..1417245 (-) 1089 WP_017061124.1 3-dehydroquinate synthase -
  ITG10_RS06375 (ITG10_06305) aroK 1417271..1417789 (-) 519 WP_009848907.1 shikimate kinase AroK -
  ITG10_RS06380 (ITG10_06310) pilQ 1417966..1419684 (-) 1719 WP_128644404.1 type IV pilus secretin PilQ family protein Machinery gene
  ITG10_RS06385 (ITG10_06315) pilP 1419720..1420238 (-) 519 WP_017066198.1 pilus assembly protein PilP Machinery gene
  ITG10_RS06390 (ITG10_06320) pilO 1420225..1420836 (-) 612 WP_017632095.1 type 4a pilus biogenesis protein PilO Machinery gene
  ITG10_RS06395 (ITG10_06325) pilN 1420829..1421428 (-) 600 WP_017632094.1 PilN domain-containing protein Machinery gene
  ITG10_RS06400 (ITG10_06330) pilM 1421412..1422437 (-) 1026 WP_017632093.1 type IV pilus assembly protein PilM Machinery gene
  ITG10_RS06405 (ITG10_06335) - 1422589..1425150 (+) 2562 WP_164913310.1 PBP1A family penicillin-binding protein -

Sequence

Protein


Download         Length: 203 a.a.        Molecular weight: 23609.00 Da        Isoelectric Point: 4.4119

>NTDB_id=517875 ITG10_RS06390 WP_017632095.1 1420225..1420836(-) (pilO) [Vibrio sp. ED004]
MANLQNWMSFQDLDVDEITEWPLLPQLLVILVLMVLIQGVGTWLYVLPLDDELQQMKQQEQTLKTTLRIKANKVAALPKL
QSQLDELTSRYDYLLEQLPVQKELASMLASVNELGLDNSLTFTRIDWGQKQNKEFLYRLPLNIELTGDYHEIGDFSAAIA
KLPRIISFDDVNWQRVSQESSTLHFRVRAYTYQFKSEVNDETQ

Nucleotide


Download         Length: 612 bp        

>NTDB_id=517875 ITG10_RS06390 WP_017632095.1 1420225..1420836(-) (pilO) [Vibrio sp. ED004]
ATGGCTAACCTTCAAAATTGGATGAGTTTTCAGGATCTTGATGTTGATGAGATCACTGAGTGGCCGTTGCTGCCCCAGCT
CTTAGTCATTCTGGTATTGATGGTGTTAATTCAAGGTGTCGGCACTTGGCTTTATGTGTTGCCACTCGACGATGAACTGC
AACAAATGAAGCAACAAGAACAAACCTTAAAGACGACCTTGCGAATAAAGGCCAACAAGGTTGCGGCCTTACCTAAATTG
CAGAGCCAGTTGGATGAACTAACCAGTCGCTACGATTATCTGTTGGAGCAGCTGCCCGTTCAAAAAGAACTGGCCAGTAT
GTTGGCGTCCGTGAATGAACTCGGCTTGGATAATTCGCTGACTTTCACTCGAATCGATTGGGGACAAAAGCAGAACAAGG
AGTTCCTCTATCGTTTACCGCTCAATATCGAACTGACGGGTGACTACCATGAGATTGGCGACTTCTCTGCGGCCATCGCC
AAGCTGCCGCGCATCATTAGCTTTGATGATGTGAACTGGCAGCGAGTTAGCCAAGAAAGCAGTACGCTGCACTTTAGGGT
TCGAGCTTATACCTACCAGTTTAAATCGGAGGTCAATGATGAAACCCAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(53-195)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

76.166

95.074

0.724
  pilO Vibrio campbellii strain DS40M4

72.105

93.596

0.675