Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   LLNZ_RS10615 Genome accession   NC_017949
Coordinates   2100371..2100796 (-) Length   141 a.a.
NCBI ID   WP_011835834.1    Uniprot ID   A2RN06
Organism   Lactococcus cremoris subsp. cremoris NZ9000     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 2056108..2108296 2100371..2100796 within 0


Gene organization within MGE regions


Location: 2056108..2108296
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LLNZ_RS10330 (LLNZ_10670) macP 2056108..2056386 (-) 279 WP_011676893.1 cell wall synthase accessory phosphoprotein MacP -
  LLNZ_RS10335 (LLNZ_10675) - 2056379..2056963 (-) 585 WP_011835788.1 NUDIX hydrolase -
  LLNZ_RS10340 (LLNZ_10680) glmU 2057064..2058440 (-) 1377 WP_011835789.1 bifunctional UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase GlmU -
  LLNZ_RS10345 (LLNZ_10685) proC 2058661..2059452 (+) 792 WP_011835790.1 pyrroline-5-carboxylate reductase -
  LLNZ_RS10350 (LLNZ_10690) rpsO 2059536..2059805 (-) 270 WP_010906184.1 30S ribosomal protein S15 -
  LLNZ_RS10355 (LLNZ_10695) pknB 2059976..2061853 (-) 1878 WP_011835791.1 Stk1 family PASTA domain-containing Ser/Thr kinase -
  LLNZ_RS10360 (LLNZ_10700) - 2061853..2062629 (-) 777 WP_011835792.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  LLNZ_RS10365 (LLNZ_10705) rsmB 2062752..2064026 (-) 1275 WP_011835793.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  LLNZ_RS10375 (LLNZ_10710) - 2065234..2066091 (-) 858 WP_011835794.1 GH25 family lysozyme -
  LLNZ_RS10380 (LLNZ_10715) - 2066093..2066554 (-) 462 WP_011835795.1 phage holin -
  LLNZ_RS10385 (LLNZ_10720) - 2066570..2066917 (-) 348 WP_011835796.1 hypothetical protein -
  LLNZ_RS10390 (LLNZ_10725) - 2066930..2067166 (-) 237 WP_011835797.1 hypothetical protein -
  LLNZ_RS10395 (LLNZ_10730) - 2067182..2072035 (-) 4854 WP_011835798.1 serine/threonine protein phosphatase -
  LLNZ_RS13490 - 2072032..2072976 (-) 945 WP_050750512.1 hypothetical protein -
  LLNZ_RS10410 - 2073038..2073685 (-) 648 WP_011835800.1 distal tail protein Dit -
  LLNZ_RS10415 (LLNZ_10745) - 2073682..2078829 (-) 5148 WP_011835801.1 phage tail tape measure protein -
  LLNZ_RS10420 (LLNZ_10750) - 2079052..2079468 (-) 417 WP_011835802.1 phage tail assembly chaperone -
  LLNZ_RS10425 (LLNZ_10755) - 2079613..2080206 (-) 594 WP_011835803.1 phage tail protein -
  LLNZ_RS10430 (LLNZ_10760) - 2080237..2080632 (-) 396 WP_011835804.1 DUF806 family protein -
  LLNZ_RS10435 (LLNZ_10765) - 2080629..2081135 (-) 507 WP_011835805.1 HK97 gp10 family phage protein -
  LLNZ_RS10440 (LLNZ_10770) - 2081137..2081487 (-) 351 WP_011835806.1 phage head closure protein -
  LLNZ_RS10445 (LLNZ_10775) - 2081462..2081785 (-) 324 WP_011835807.1 head-tail connector protein -
  LLNZ_RS10450 (LLNZ_10780) - 2081986..2083200 (-) 1215 WP_011835808.1 phage major capsid protein -
  LLNZ_RS10455 (LLNZ_10785) - 2083212..2083916 (-) 705 WP_002819795.1 head maturation protease, ClpP-related -
  LLNZ_RS10460 (LLNZ_10790) - 2083962..2085140 (-) 1179 WP_014735131.1 phage portal protein -
  LLNZ_RS10465 (LLNZ_10795) - 2085137..2085346 (-) 210 WP_011835810.1 DUF1056 family protein -
  LLNZ_RS10470 (LLNZ_10800) - 2085315..2086949 (-) 1635 WP_231097782.1 terminase large subunit -
  LLNZ_RS10475 - 2087002..2088177 (-) 1176 Protein_2048 IS256-like element IS905 family transposase -
  LLNZ_RS13495 (LLNZ_10815) - 2088271..2088546 (-) 276 Protein_2049 terminase large subunit -
  LLNZ_RS10485 (LLNZ_10820) - 2088536..2088934 (-) 399 WP_011835812.1 phage terminase small subunit P27 family -
  LLNZ_RS10490 (LLNZ_10825) - 2089116..2089634 (-) 519 WP_011835813.1 HNH endonuclease -
  LLNZ_RS10495 (LLNZ_10830) - 2089638..2089928 (-) 291 WP_011835814.1 hypothetical protein -
  LLNZ_RS10505 (LLNZ_10835) - 2090305..2090706 (-) 402 WP_011835815.1 DUF722 domain-containing protein -
  LLNZ_RS10510 (LLNZ_10840) - 2090789..2090974 (-) 186 WP_011835816.1 hypothetical protein -
  LLNZ_RS10515 (LLNZ_10845) - 2090971..2091279 (-) 309 WP_011835817.1 hypothetical protein -
  LLNZ_RS10520 (LLNZ_10850) - 2091281..2091460 (-) 180 WP_014735134.1 hypothetical protein -
  LLNZ_RS13965 (LLNZ_10855) - 2091462..2091596 (-) 135 WP_259285540.1 hypothetical protein -
  LLNZ_RS10525 (LLNZ_10860) istB 2091711..2092469 (-) 759 WP_003331414.1 IS21-like element IS712 family helper ATPase IstB -
  LLNZ_RS10530 (LLNZ_10865) istA 2092481..2093704 (-) 1224 WP_003331415.1 IS21-like element IS712 family transposase -
  LLNZ_RS13660 (LLNZ_10870) - 2093760..2093903 (-) 144 WP_014735135.1 hypothetical protein -
  LLNZ_RS10535 (LLNZ_10875) - 2094264..2094473 (+) 210 WP_011835819.1 hypothetical protein -
  LLNZ_RS10540 (LLNZ_10880) - 2094522..2094722 (-) 201 WP_014735136.1 DUF1660 domain-containing protein -
  LLNZ_RS10545 (LLNZ_10885) - 2094719..2094949 (-) 231 WP_011835820.1 hypothetical protein -
  LLNZ_RS10550 (LLNZ_10890) - 2094968..2095306 (-) 339 WP_011835821.1 DUF1140 family protein -
  LLNZ_RS10555 (LLNZ_10895) dut 2095307..2095726 (-) 420 WP_011835822.1 dUTP diphosphatase -
  LLNZ_RS10560 (LLNZ_10900) - 2095723..2096280 (-) 558 WP_011835823.1 DUF1642 domain-containing protein -
  LLNZ_RS10565 (LLNZ_10905) - 2096296..2096532 (-) 237 WP_011835824.1 hypothetical protein -
  LLNZ_RS10570 (LLNZ_10910) - 2096513..2096707 (-) 195 WP_014735137.1 hypothetical protein -
  LLNZ_RS10575 (LLNZ_10915) - 2096704..2096901 (-) 198 WP_011835825.1 DUF1125 domain-containing protein -
  LLNZ_RS10580 (LLNZ_10920) - 2096902..2097168 (-) 267 WP_011835826.1 hypothetical protein -
  LLNZ_RS10585 (LLNZ_10925) - 2097149..2097481 (-) 333 WP_011835827.1 hypothetical protein -
  LLNZ_RS13970 - 2097496..2097780 (-) 285 WP_011835828.1 hypothetical protein -
  LLNZ_RS13975 - 2097797..2098021 (-) 225 WP_011835829.1 hypothetical protein -
  LLNZ_RS10595 (LLNZ_10940) - 2098128..2098367 (-) 240 WP_011835830.1 DUF1031 domain-containing protein -
  LLNZ_RS10600 (LLNZ_10945) - 2098364..2098738 (-) 375 WP_011835831.1 DUF1064 domain-containing protein -
  LLNZ_RS10605 (LLNZ_10950) - 2098735..2099460 (-) 726 WP_011835832.1 hypothetical protein -
  LLNZ_RS10610 (LLNZ_10955) - 2099460..2100242 (-) 783 WP_011835833.1 phage replisome organiser protein -
  LLNZ_RS10615 (LLNZ_10960) ssb 2100371..2100796 (-) 426 WP_011835834.1 single-stranded DNA-binding protein Machinery gene
  LLNZ_RS10620 (LLNZ_10965) - 2100789..2100923 (-) 135 WP_011835835.1 translation initiation factor -
  LLNZ_RS10630 (LLNZ_10975) - 2101176..2101547 (-) 372 WP_011835836.1 Rad52/Rad22 family DNA repair protein -
  LLNZ_RS10635 (LLNZ_10980) - 2101556..2101951 (-) 396 WP_011835837.1 hypothetical protein -
  LLNZ_RS10640 (LLNZ_10985) - 2102050..2102292 (-) 243 WP_011835838.1 hypothetical protein -
  LLNZ_RS10645 (LLNZ_10990) - 2102379..2102777 (+) 399 WP_187287215.1 DUF2513 domain-containing protein -
  LLNZ_RS10650 (LLNZ_10995) - 2102770..2102973 (-) 204 WP_011835840.1 hypothetical protein -
  LLNZ_RS10655 (LLNZ_11000) - 2103069..2103338 (-) 270 WP_011835841.1 hypothetical protein -
  LLNZ_RS10660 (LLNZ_11005) - 2103352..2104110 (-) 759 WP_011835842.1 phage repressor protein/antirepressor Ant -
  LLNZ_RS10665 (LLNZ_11010) - 2104123..2104371 (-) 249 WP_011835843.1 helix-turn-helix transcriptional regulator -
  LLNZ_RS10670 (LLNZ_11015) - 2104476..2104712 (+) 237 WP_011835844.1 hypothetical protein -
  LLNZ_RS10675 (LLNZ_11020) - 2104684..2104905 (-) 222 WP_011835845.1 hypothetical protein -
  LLNZ_RS10680 (LLNZ_11030) - 2105280..2105477 (-) 198 WP_014735140.1 hypothetical protein -
  LLNZ_RS10685 (LLNZ_11035) - 2105728..2106258 (+) 531 WP_374930652.1 helix-turn-helix domain-containing protein -
  LLNZ_RS10690 (LLNZ_11040) - 2106264..2106698 (+) 435 WP_011835848.1 zinc peptidase -
  LLNZ_RS10695 (LLNZ_11045) - 2106712..2107047 (+) 336 WP_011835849.1 hypothetical protein -
  LLNZ_RS10700 (LLNZ_11050) - 2107115..2108296 (+) 1182 WP_014735141.1 site-specific integrase -

Sequence


Protein


Download         Length: 141 a.a.        Molecular weight: 15685.50 Da        Isoelectric Point: 6.9813

>NTDB_id=51426 LLNZ_RS10615 WP_011835834.1 2100371..2100796(-) (ssb) [Lactococcus cremoris subsp. cremoris NZ9000]
MINNVTLVGRITKKPELRYTPQNKAVATFTLAVNRAFKNANGEREADFISCVIWGKSAENLANWTHKGQLIGVIGNIQTR
NYENQQGQRVYITEVVASNFQVLEKSNQANGERVSNPAAKPQNNDSFGSDPMEISDDDLPF

Nucleotide


Download         Length: 426 bp        

>NTDB_id=51426 LLNZ_RS10615 WP_011835834.1 2100371..2100796(-) (ssb) [Lactococcus cremoris subsp. cremoris NZ9000]
ATGATTAACAATGTCACTCTAGTAGGGCGAATCACTAAAAAACCTGAACTTAGATATACACCACAAAATAAAGCAGTTGC
CACTTTTACTCTTGCAGTTAATCGAGCATTTAAAAATGCTAATGGAGAAAGAGAAGCTGACTTTATCAGTTGTGTTATTT
GGGGTAAATCAGCCGAAAACTTGGCCAATTGGACTCATAAAGGTCAATTAATTGGAGTTATTGGGAATATACAGACTCGA
AACTATGAGAACCAACAAGGGCAGCGTGTTTATATTACGGAGGTTGTCGCAAGTAATTTCCAAGTACTAGAAAAAAGTAA
TCAAGCAAATGGTGAACGAGTTAGTAATCCAGCTGCAAAACCACAAAATAACGATTCTTTTGGAAGTGATCCAATGGAAA
TTTCAGATGATGACCTACCATTCTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A2RN06

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Latilactobacillus sakei subsp. sakei 23K

53.529

100

0.645

  ssbA Bacillus subtilis subsp. subtilis str. 168

51.744

100

0.631

  ssbB/cilA Streptococcus pneumoniae TIGR4

43.262

100

0.433

  ssbB Streptococcus sobrinus strain NIDR 6715-7

43.262

100

0.433

  ssbB/cilA Streptococcus mitis NCTC 12261

43.262

100

0.433

  ssbB/cilA Streptococcus pneumoniae Rx1

42.553

100

0.426

  ssbB/cilA Streptococcus pneumoniae D39

42.553

100

0.426

  ssbB/cilA Streptococcus pneumoniae R6

42.553

100

0.426

  ssbB/cilA Streptococcus mitis SK321

42.553

100

0.426

  ssbB Bacillus subtilis subsp. subtilis str. 168

56.731

73.759

0.418

  ssbA Streptococcus mutans UA159

39.007

100

0.39

  ssbB Lactococcus lactis subsp. cremoris KW2

51.456

73.05

0.376


Multiple sequence alignment