Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   I6G23_RS03450 Genome accession   NZ_CP065735
Coordinates   417851..418789 (+) Length   312 a.a.
NCBI ID   WP_010906320.1    Uniprot ID   A0AAC9W673
Organism   Lactococcus lactis strain FDAARGOS_866     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 412851..423789
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6G23_RS03450 (I6G23_03450) comGA 417851..418789 (+) 939 WP_010906320.1 competence type IV pilus ATPase ComGA Machinery gene
  I6G23_RS03455 (I6G23_03455) comGB 418683..419756 (+) 1074 WP_010906319.1 competence type IV pilus assembly protein ComGB Machinery gene
  I6G23_RS03460 (I6G23_03460) comGC 419884..420153 (+) 270 WP_023349160.1 competence type IV pilus major pilin ComGC Machinery gene
  I6G23_RS03465 (I6G23_03465) comGD 420128..420544 (+) 417 WP_021216554.1 competence type IV pilus minor pilin ComGD Machinery gene
  I6G23_RS03470 (I6G23_03470) comGE 420516..420812 (+) 297 WP_010906316.1 competence type IV pilus minor pilin ComGE Machinery gene
  I6G23_RS03475 (I6G23_03475) comGF 420775..421221 (+) 447 WP_031296844.1 competence type IV pilus minor pilin ComGF Machinery gene
  I6G23_RS03480 (I6G23_03480) comGG 421269..421544 (+) 276 WP_257012268.1 hypothetical protein Machinery gene
  I6G23_RS03485 (I6G23_03485) - 421625..422062 (+) 438 WP_010906313.1 zinc-dependent MarR family transcriptional regulator -
  I6G23_RS03490 (I6G23_03490) - 422059..422901 (+) 843 WP_010906312.1 metal ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 312 a.a.        Molecular weight: 35719.89 Da        Isoelectric Point: 6.1804

>NTDB_id=513660 I6G23_RS03450 WP_010906320.1 417851..418789(+) (comGA) [Lactococcus lactis strain FDAARGOS_866]
MIPKMAKELIQRAIDRDASDIYLIASKEKYKLYFRQMTARDLVEEIGLEVGLALLTHFKFLAGMNTGERRRVQLGACWYE
IEESNAKRLRLSTVGDFEGNESLVIRLLHDQNKELDFWFDQELENYDCKRGLHLFAGPVGSGKTSLMFDLARRHFTDLQV
INIEEPVELIDSDFIQLQVNEVIGNSYDELIKLSLRHRPDLLIVGEIRDKLTAQAVLRASLTGYTVFSTVHASSVRGVIQ
RLSELGLSHWEIKNALSSVVYQRLIAGKGVLDIAKNEFESWSSENWNKKIDQLLTNGYLTPTEAKREKIKTD

Nucleotide


Download         Length: 939 bp        

>NTDB_id=513660 I6G23_RS03450 WP_010906320.1 417851..418789(+) (comGA) [Lactococcus lactis strain FDAARGOS_866]
ATGATACCAAAAATGGCAAAAGAATTAATTCAAAGAGCAATTGATAGAGATGCATCAGATATTTATCTGATTGCTTCAAA
AGAAAAGTATAAACTTTATTTTCGACAAATGACAGCACGTGATCTGGTAGAAGAAATTGGGTTAGAAGTTGGCTTAGCAC
TACTTACTCACTTTAAGTTTCTAGCTGGGATGAATACAGGAGAACGCAGACGAGTTCAATTGGGAGCTTGTTGGTATGAA
ATAGAGGAAAGTAATGCAAAACGTTTGCGTCTTTCGACAGTGGGGGATTTCGAGGGTAATGAATCTTTAGTAATACGTCT
CTTGCATGATCAAAACAAAGAACTTGATTTTTGGTTTGATCAAGAGCTAGAGAATTATGATTGTAAAAGAGGATTGCATC
TCTTTGCCGGTCCAGTAGGCTCGGGAAAAACATCTTTAATGTTTGATTTAGCTCGTCGTCATTTTACAGATTTACAAGTT
ATCAATATAGAAGAACCCGTTGAGTTGATTGATTCCGATTTTATTCAGTTACAAGTCAACGAAGTCATTGGTAATAGTTA
TGATGAATTAATTAAATTATCACTGAGGCATCGCCCAGATTTGTTAATCGTTGGTGAAATTCGTGATAAATTGACCGCTC
AAGCAGTTTTACGGGCTAGTTTGACAGGTTATACAGTGTTTTCAACCGTTCATGCCTCTTCTGTTAGAGGTGTCATTCAG
AGATTATCAGAGCTGGGTTTAAGTCATTGGGAAATAAAAAATGCTTTATCAAGTGTTGTTTATCAGCGTTTAATAGCTGG
GAAAGGAGTATTAGACATTGCCAAAAACGAATTTGAGTCTTGGTCATCAGAAAATTGGAATAAAAAAATTGATCAGCTAC
TTACAAATGGATATCTCACACCTACTGAAGCCAAAAGGGAAAAAATTAAGACTGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Lactococcus lactis subsp. cremoris KW2

76.603

100

0.766
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

54.662

99.679

0.545
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

53.055

99.679

0.529
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

52.09

99.679

0.519
  comGA/cglA/cilD Streptococcus pneumoniae D39

52.09

99.679

0.519
  comGA/cglA/cilD Streptococcus pneumoniae R6

52.09

99.679

0.519
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

52.09

99.679

0.519
  comYA Streptococcus gordonii str. Challis substr. CH1

51.923

100

0.519
  comYA Streptococcus mutans UA140

51.923

100

0.519
  comYA Streptococcus mutans UA159

51.923

100

0.519