Detailed information NTG0513661

Bioinformatically predicted

Overview

• Name: comGB
• Type: Machinery gene
• Locus tag: I6G23_RS03455
• Genome accession: NZ_CP065735
• Coordinates: 418683..419756 (+)
• Length: 357 a.a.
• NCBI ID: WP_010906319.1
• Uniprot ID: A0A552YT77
• Organism: Lactococcus lactis strain FDAARGOS_866
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 413683..424756

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I6G23_RS03450 (I6G23_03450)	comGA	417851..418789 (+)	939	WP_010906320.1	competence type IV pilus ATPase ComGA	Machinery gene
I6G23_RS03455 (I6G23_03455)	comGB	418683..419756 (+)	1074	WP_010906319.1	competence type IV pilus assembly protein ComGB	Machinery gene
I6G23_RS03460 (I6G23_03460)	comGC	419884..420153 (+)	270	WP_023349160.1	competence type IV pilus major pilin ComGC	Machinery gene
I6G23_RS03465 (I6G23_03465)	comGD	420128..420544 (+)	417	WP_021216554.1	competence type IV pilus minor pilin ComGD	Machinery gene
I6G23_RS03470 (I6G23_03470)	comGE	420516..420812 (+)	297	WP_010906316.1	competence type IV pilus minor pilin ComGE	Machinery gene
I6G23_RS03475 (I6G23_03475)	comGF	420775..421221 (+)	447	WP_031296844.1	competence type IV pilus minor pilin ComGF	Machinery gene
I6G23_RS03480 (I6G23_03480)	comGG	421269..421544 (+)	276	WP_257012268.1	hypothetical protein	Machinery gene
I6G23_RS03485 (I6G23_03485)	-	421625..422062 (+)	438	WP_010906313.1	zinc-dependent MarR family transcriptional regulator	-
I6G23_RS03490 (I6G23_03490)	-	422059..422901 (+)	843	WP_010906312.1	metal ABC transporter substrate-binding protein	-
I6G23_RS03495 (I6G23_03495)	-	423078..423815 (+)	738	WP_010906311.1	metal ABC transporter ATP-binding protein	-
I6G23_RS03500 (I6G23_03500)	-	423808..424617 (+)	810	WP_010906310.1	metal ABC transporter permease	-

Sequence

Protein

• Length: 357 a.a.
• Molecular weight: 40330.51 Da
• Isoelectric Point: 10.3000

>NTDB_id=513661 I6G23_RS03455 WP_010906319.1 418683..419756(+) (comGB) [Lactococcus lactis strain FDAARGOS_866]
MSLGHQKIGIKKLISYLQMDISHLLKPKGKKLRLIKQAKLIQLMGNLLNSGFNLGEVINFLSLSKLVEKEFTLKMKEGLA
SGQALSELLESLSFSKNVVTQLALVEVHGNLSGTMQLVELHLKKQLKVKNKLVEVATYPILLLIFLVGIMWGLKNYLLPQ
LNSGSNFATLLINHLPLVFFSFGAFIFLLTALSVTLFKRKSAIMNFTFLVKLPLVHSFIRLYLTAYFAREWGNLIAQGVE
LRQIINLMKKQKSRIFSEVGKNLDLELNAGRSFEQAVSKLALFLPELSLMIEYGAIKDKLGLELSLYADECWEQFFTKID
RLMQLIQPLVFIFVALMIILLYAAMLLPIYSNMGSGI

Nucleotide

• Length: 1074 bp

>NTDB_id=513661 I6G23_RS03455 WP_010906319.1 418683..419756(+) (comGB) [Lactococcus lactis strain FDAARGOS_866]
TTGAGTCTTGGTCATCAGAAAATTGGAATAAAAAAATTGATCAGCTACTTACAAATGGATATCTCACACCTACTGAAGCC
AAAAGGGAAAAAATTAAGACTGATTAAACAAGCCAAACTTATCCAACTGATGGGAAATCTTTTGAATAGTGGATTTAATT
TAGGAGAAGTGATAAATTTCTTATCTTTATCAAAACTTGTGGAAAAAGAATTTACATTAAAAATGAAAGAAGGTCTCGCT
TCTGGTCAAGCTTTATCAGAGCTTTTAGAAAGTCTTTCATTTTCTAAAAATGTGGTGACACAACTTGCTTTGGTAGAAGT
GCATGGTAACTTGTCAGGGACGATGCAATTAGTTGAACTTCATCTAAAAAAACAGCTTAAAGTTAAAAATAAATTAGTGG
AAGTTGCTACTTATCCAATATTGTTATTAATTTTTCTGGTTGGAATTATGTGGGGCTTAAAAAATTATTTACTACCACAA
CTCAATAGTGGCAGTAACTTTGCTACTCTATTGATTAATCATTTACCCTTAGTATTTTTTTCGTTTGGAGCTTTTATATT
TTTACTGACAGCTTTGTCAGTAACTCTTTTTAAACGAAAATCAGCAATAATGAATTTTACATTTCTTGTTAAATTACCTC
TGGTTCATTCTTTTATCCGTTTATATTTAACAGCTTACTTTGCAAGAGAATGGGGAAATCTAATTGCTCAAGGTGTTGAA
TTACGTCAAATTATTAACTTGATGAAAAAACAAAAAAGTCGAATTTTTTCAGAAGTTGGCAAGAATCTTGATTTAGAATT
GAATGCTGGTCGGTCTTTTGAACAAGCTGTCAGTAAACTTGCGCTTTTTTTACCCGAGCTCTCGTTAATGATTGAATATG
GAGCTATTAAAGATAAATTAGGCTTAGAGCTTTCGCTTTATGCGGATGAATGTTGGGAACAATTTTTTACAAAGATAGAT
CGTTTAATGCAATTAATCCAACCTCTTGTTTTTATTTTCGTAGCATTGATGATTATTTTGCTATATGCAGCCATGTTACT
CCCCATCTATTCAAATATGGGTTCGGGAATATAG

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein F (T2SSF) (227-348)

• Type II secretion system (T2SS), protein F (T2SSF) (41-159)

3D structure

• AlphaFold DB: A0A552YT77

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGB	Lactococcus lactis subsp. cremoris KW2	83.578	95.518	0.798
comYB	Streptococcus gordonii str. Challis substr. CH1	53.959	95.518	0.515
comYB	Streptococcus mutans UA140	53.372	95.518	0.51
comYB	Streptococcus mutans UA159	53.079	95.518	0.507
comGB/cglB	Streptococcus mitis SK321	51.343	93.838	0.482
comGB/cglB	Streptococcus pneumoniae TIGR4	51.045	93.838	0.479
comGB/cglB	Streptococcus pneumoniae Rx1	51.045	93.838	0.479
comGB/cglB	Streptococcus pneumoniae D39	51.045	93.838	0.479
comGB/cglB	Streptococcus pneumoniae R6	51.045	93.838	0.479
comGB/cglB	Streptococcus mitis NCTC 12261	50.448	93.838	0.473