Detailed information NTG0513665

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: I6G23_RS03475
• Genome accession: NZ_CP065735
• Coordinates: 420775..421221 (+)
• Length: 148 a.a.
• NCBI ID: WP_031296844.1
• Uniprot ID: A0AAC9R3D6
• Organism: Lactococcus lactis strain FDAARGOS_866
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 415775..426221

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I6G23_RS03450 (I6G23_03450)	comGA	417851..418789 (+)	939	WP_010906320.1	competence type IV pilus ATPase ComGA	Machinery gene
I6G23_RS03455 (I6G23_03455)	comGB	418683..419756 (+)	1074	WP_010906319.1	competence type IV pilus assembly protein ComGB	Machinery gene
I6G23_RS03460 (I6G23_03460)	comGC	419884..420153 (+)	270	WP_023349160.1	competence type IV pilus major pilin ComGC	Machinery gene
I6G23_RS03465 (I6G23_03465)	comGD	420128..420544 (+)	417	WP_021216554.1	competence type IV pilus minor pilin ComGD	Machinery gene
I6G23_RS03470 (I6G23_03470)	comGE	420516..420812 (+)	297	WP_010906316.1	competence type IV pilus minor pilin ComGE	Machinery gene
I6G23_RS03475 (I6G23_03475)	comGF	420775..421221 (+)	447	WP_031296844.1	competence type IV pilus minor pilin ComGF	Machinery gene
I6G23_RS03480 (I6G23_03480)	comGG	421269..421544 (+)	276	WP_257012268.1	hypothetical protein	Machinery gene
I6G23_RS03485 (I6G23_03485)	-	421625..422062 (+)	438	WP_010906313.1	zinc-dependent MarR family transcriptional regulator	-
I6G23_RS03490 (I6G23_03490)	-	422059..422901 (+)	843	WP_010906312.1	metal ABC transporter substrate-binding protein	-
I6G23_RS03495 (I6G23_03495)	-	423078..423815 (+)	738	WP_010906311.1	metal ABC transporter ATP-binding protein	-
I6G23_RS03500 (I6G23_03500)	-	423808..424617 (+)	810	WP_010906310.1	metal ABC transporter permease	-
I6G23_RS03505 (I6G23_03505)	-	424656..425522 (-)	867	WP_010906309.1	RluA family pseudouridine synthase	-
I6G23_RS03510 (I6G23_03510)	-	425728..426105 (+)	378	WP_003129983.1	pyridoxamine 5'-phosphate oxidase family protein	-

Sequence

Protein

• Length: 148 a.a.
• Molecular weight: 17083.85 Da
• Isoelectric Point: 8.6780

>NTDB_id=513665 I6G23_RS03475 WP_031296844.1 420775..421221(+) (comGF) [Lactococcus lactis strain FDAARGOS_866]
MERKLCDLNFKVKAFTLLECLVALLAISGSALVISGLTKMLKEQVAISQSDSIKDWQIFCQQMRFELSGTKLDRVEQNFL
YVTKDKKLRFGFMSDDFRKTDDKGQGYQPMLYDIKAAKIQATKNLITIRIDFNQGGERTFIYQFPEDT

Nucleotide

• Length: 447 bp

>NTDB_id=513665 I6G23_RS03475 WP_031296844.1 420775..421221(+) (comGF) [Lactococcus lactis strain FDAARGOS_866]
ATGGAAAGGAAATTATGCGACTTGAACTTCAAAGTTAAAGCATTTACCTTGTTGGAGTGTTTGGTGGCACTTCTTGCAAT
TTCTGGTTCAGCCCTTGTCATCTCAGGTTTAACAAAGATGTTGAAAGAACAAGTGGCGATTAGTCAAAGTGATAGCATAA
AAGATTGGCAAATTTTCTGTCAGCAAATGCGTTTTGAACTCTCAGGAACAAAATTAGATAGGGTAGAACAGAATTTTCTG
TATGTAACTAAAGATAAAAAACTAAGGTTTGGTTTTATGAGTGATGATTTCCGGAAGACTGATGACAAAGGTCAGGGTTA
TCAGCCAATGCTTTATGATATAAAAGCGGCTAAGATTCAAGCTACTAAAAATTTAATAACCATAAGAATTGATTTTAATC
AAGGAGGTGAAAGAACATTTATTTATCAATTTCCAGAAGATACGTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (48-137)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Lactococcus lactis subsp. cremoris KW2	75.54	93.919	0.709
comYF	Streptococcus mutans UA140	47.518	95.27	0.453
comYF	Streptococcus mutans UA159	46.809	95.27	0.446
comGF/cglF	Streptococcus mitis SK321	43.066	92.568	0.399
comGF/cglF	Streptococcus pneumoniae Rx1	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae D39	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae R6	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae TIGR4	40.426	95.27	0.385
comGF/cglF	Streptococcus mitis NCTC 12261	41.606	92.568	0.385