Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   I6G96_RS09560 Genome accession   NZ_CP065627
Coordinates   2126679..2127122 (-) Length   147 a.a.
NCBI ID   WP_197944764.1    Uniprot ID   -
Organism   Delftia acidovorans strain FDAARGOS_939     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 2112151..2126493 2126679..2127122 flank 186


Gene organization within MGE regions


Location: 2112151..2127122
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6G96_RS09495 (I6G96_09495) - 2112151..2112882 (+) 732 WP_197944755.1 class I SAM-dependent methyltransferase -
  I6G96_RS09500 (I6G96_09500) - 2112917..2114632 (+) 1716 WP_197944756.1 ABC transporter ATP-binding protein -
  I6G96_RS09505 (I6G96_09505) - 2114992..2115567 (+) 576 WP_197944757.1 GNAT family N-acetyltransferase -
  I6G96_RS09510 (I6G96_09510) - 2115575..2116849 (+) 1275 WP_197944758.1 glycosyltransferase family 61 protein -
  I6G96_RS09515 (I6G96_09515) - 2116846..2117952 (+) 1107 WP_332259254.1 DegT/DnrJ/EryC1/StrS family aminotransferase -
  I6G96_RS09520 (I6G96_09520) - 2117972..2118385 (+) 414 WP_097205450.1 sugar 3,4-ketoisomerase -
  I6G96_RS09525 (I6G96_09525) - 2118372..2119403 (+) 1032 WP_197944759.1 glycosyltransferase -
  I6G96_RS09530 (I6G96_09530) - 2119403..2120329 (+) 927 WP_097204153.1 glycosyltransferase family 2 protein -
  I6G96_RS09535 (I6G96_09535) glf 2120326..2121438 (+) 1113 WP_197944760.1 UDP-galactopyranose mutase -
  I6G96_RS09540 (I6G96_09540) - 2121446..2122594 (+) 1149 WP_232245285.1 glycosyltransferase family 4 protein -
  I6G96_RS09545 (I6G96_09545) - 2122599..2123816 (-) 1218 WP_197944761.1 glycosyltransferase -
  I6G96_RS09550 (I6G96_09550) - 2123813..2124664 (-) 852 WP_197944762.1 glycosyltransferase family 2 protein -
  I6G96_RS09555 (I6G96_09555) - 2124661..2126613 (-) 1953 WP_197944763.1 tetratricopeptide repeat protein -
  I6G96_RS09560 (I6G96_09560) pilA2 2126679..2127122 (-) 444 WP_197944764.1 pilin Machinery gene

Sequence

Protein


Download         Length: 147 a.a.        Molecular weight: 15391.66 Da        Isoelectric Point: 9.8925

>NTDB_id=512285 I6G96_RS09560 WP_197944764.1 2126679..2127122(-) (pilA2) [Delftia acidovorans strain FDAARGOS_939]
MKRSIQQGFTLIELMIVVAIIGILAAVALPAYQDYTVRSKVSEGLTLAASAKTAVSEHRLSQGTWPTTSAAAGYTSPNTK
YVNGITINNGVITITYKTGSSGSGVATSATDLVLSPLSVVGAVDWRCKRPATTPVDAKYLPANCRQS

Nucleotide


Download         Length: 444 bp        

>NTDB_id=512285 I6G96_RS09560 WP_197944764.1 2126679..2127122(-) (pilA2) [Delftia acidovorans strain FDAARGOS_939]
ATGAAGCGTTCCATCCAGCAAGGTTTTACCTTGATCGAACTGATGATCGTCGTGGCGATCATCGGCATTTTGGCAGCCGT
TGCGCTGCCGGCTTATCAGGATTACACGGTGCGTTCCAAGGTGTCTGAAGGCCTGACACTGGCAGCCTCTGCCAAGACTG
CCGTTTCTGAACACCGCCTGAGCCAAGGTACTTGGCCTACCACCAGCGCAGCCGCAGGCTACACCTCCCCCAATACAAAG
TATGTGAACGGCATCACCATTAATAATGGCGTCATTACCATTACATACAAGACTGGCTCCTCTGGCTCTGGCGTCGCCAC
CAGCGCCACCGATCTGGTGCTCTCCCCCCTGTCCGTCGTGGGTGCTGTGGACTGGAGGTGCAAGCGTCCTGCAACGACCC
CTGTGGATGCCAAATACCTGCCGGCCAACTGCCGTCAAAGCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(4-29)

Pilin (bacterial filament) (Pilin)

(37-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

55.944

97.279

0.544
  pilA2 Legionella pneumophila strain ERS1305867

55.245

97.279

0.537
  pilE Neisseria gonorrhoeae MS11

45

100

0.49
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

36.702

100

0.469
  pilA Ralstonia pseudosolanacearum GMI1000

41.818

100

0.469
  comP Acinetobacter baylyi ADP1

46.259

100

0.463
  pilE Neisseria gonorrhoeae strain FA1090

42.5

100

0.463
  pilA/pilAI Pseudomonas stutzeri DSM 10701

42.282

100

0.429
  pilA/pilAII Pseudomonas stutzeri DSM 10701

39.073

100

0.401
  pilA/pilA1 Eikenella corrodens VA1

37.107

100

0.401
  pilA Acinetobacter baumannii strain A118

39.041

99.32

0.388