Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   ST4145_RS08690 Genome accession   NZ_CP065492
Coordinates   1684351..1685292 (-) Length   313 a.a.
NCBI ID   WP_014608745.1    Uniprot ID   -
Organism   Streptococcus thermophilus strain 4145     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1679351..1690292
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ST4145_RS08650 (ST4145_08575) - 1679427..1680620 (-) 1194 WP_014608740.1 acetate kinase -
  ST4145_RS08655 (ST4145_08580) comYH 1680676..1681632 (-) 957 WP_011681684.1 class I SAM-dependent methyltransferase Machinery gene
  ST4145_RS08660 (ST4145_08585) comGG 1681677..1681994 (-) 318 WP_002952035.1 competence type IV pilus minor pilin ComGG -
  ST4145_RS08665 (ST4145_08590) comYF 1681972..1682409 (-) 438 WP_014608742.1 competence type IV pilus minor pilin ComGF Machinery gene
  ST4145_RS08670 (ST4145_08595) comGE 1682393..1682686 (-) 294 WP_011226625.1 competence type IV pilus minor pilin ComGE -
  ST4145_RS08675 (ST4145_08600) comYD 1682658..1683086 (-) 429 WP_011226626.1 competence type IV pilus minor pilin ComGD Machinery gene
  ST4145_RS08680 (ST4145_08605) comYC 1683046..1683372 (-) 327 WP_014727724.1 competence type IV pilus major pilin ComGC Machinery gene
  ST4145_RS08685 (ST4145_08610) comYB 1683369..1684469 (-) 1101 WP_120764773.1 competence type IV pilus assembly protein ComGB Machinery gene
  ST4145_RS08690 (ST4145_08615) comGA/cglA/cilD 1684351..1685292 (-) 942 WP_014608745.1 competence type IV pilus ATPase ComGA Machinery gene
  ST4145_RS08695 (ST4145_08620) - 1685373..1685735 (-) 363 WP_014608746.1 DUF1033 family protein -
  ST4145_RS08700 (ST4145_08625) rpoC 1685894..1689532 (-) 3639 WP_011226631.1 DNA-directed RNA polymerase subunit beta' -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35508.70 Da        Isoelectric Point: 6.6091

>NTDB_id=510926 ST4145_RS08690 WP_014608745.1 1684351..1685292(-) (comGA/cglA/cilD) [Streptococcus thermophilus strain 4145]
MVTEFAKEMIKNADSCGAQDIYVIPRQDNYELYMRVGQERRLIDVYRPDFMASLIGHFKFVARMMVGEKRRSQLGSCDYD
CGDGHLVSLRLSTVGDYRGLESLVIRILHSERRELVYWNQGIQPIKDALDYRGLYLFAGPVGSGKTTLMHELVQERFKGQ
QVISIEDPVEIKQDNVLQLQVNQAIDMTYDSLIKLSLRHRPDVLIIGEIRDKETARAVIRASLTGVTVLSTIHAKSIAGV
YERLLDLGVDKSELDNALQGIAYMRLIKGGGVIDFARENFQNHSSTNWNQQLEGLVKQGCLTERDIQGEKIKD

Nucleotide


Download         Length: 942 bp        

>NTDB_id=510926 ST4145_RS08690 WP_014608745.1 1684351..1685292(-) (comGA/cglA/cilD) [Streptococcus thermophilus strain 4145]
ATGGTAACAGAATTTGCTAAGGAAATGATCAAAAATGCTGATAGTTGTGGGGCTCAAGACATCTATGTCATTCCACGTCA
GGATAATTATGAGCTCTATATGCGAGTCGGGCAAGAGAGGAGATTGATTGATGTATATCGGCCTGATTTCATGGCTAGTC
TTATTGGTCACTTTAAATTTGTGGCGAGAATGATGGTGGGTGAGAAGCGTAGGAGTCAACTGGGTTCATGTGACTATGAT
TGTGGTGACGGTCACTTGGTTTCTCTGCGTTTATCAACTGTTGGAGACTATCGAGGTTTGGAGAGCTTGGTTATCCGTAT
CCTACATTCCGAACGTCGAGAATTAGTGTATTGGAATCAAGGAATCCAGCCTATTAAGGATGCCTTGGATTATAGAGGAC
TGTATCTCTTTGCGGGTCCCGTAGGTTCTGGGAAGACCACACTTATGCATGAGTTGGTTCAGGAGCGTTTTAAGGGGCAG
CAGGTAATTTCTATTGAGGATCCTGTTGAAATCAAACAGGATAATGTCTTGCAACTCCAGGTTAATCAGGCGATTGATAT
GACCTATGATAGTTTGATTAAGCTATCTCTACGTCACCGTCCAGATGTTTTGATTATTGGGGAGATTCGGGATAAGGAGA
CTGCCCGGGCAGTTATTAGAGCTAGTCTAACAGGGGTAACTGTTCTTTCAACTATTCATGCTAAGAGTATTGCTGGCGTT
TACGAACGTCTCTTGGACCTTGGCGTAGATAAGTCTGAGTTGGATAATGCTCTACAAGGCATTGCCTACATGCGTTTGAT
CAAGGGAGGAGGTGTGATTGATTTTGCCAGAGAAAATTTCCAAAACCATTCGTCGACCAACTGGAATCAGCAGTTGGAAG
GTTTGGTTAAACAAGGATGTCTCACTGAGAGGGATATCCAAGGGGAAAAAATTAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

65.273

99.361

0.649
  comYA Streptococcus mutans UA159

64.537

100

0.645
  comYA Streptococcus mutans UA140

64.537

100

0.645
  comGA/cglA/cilD Streptococcus pneumoniae D39

63.987

99.361

0.636
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

63.987

99.361

0.636
  comGA/cglA/cilD Streptococcus pneumoniae R6

63.987

99.361

0.636
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

63.987

99.361

0.636
  comYA Streptococcus gordonii str. Challis substr. CH1

62.939

100

0.629
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

61.218

99.681

0.61
  comGA Lactococcus lactis subsp. cremoris KW2

50.804

99.361

0.505
  comGA Latilactobacillus sakei subsp. sakei 23K

38.487

97.125

0.374