Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   ST4134_RS08850 Genome accession   NZ_CP065478
Coordinates   1702442..1703383 (-) Length   313 a.a.
NCBI ID   WP_022096896.1    Uniprot ID   -
Organism   Streptococcus thermophilus strain 4134     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1697442..1708383
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ST4134_RS08810 (ST4134_08780) - 1697518..1698711 (-) 1194 WP_014608740.1 acetate kinase -
  ST4134_RS08815 (ST4134_08785) comYH 1698767..1699723 (-) 957 WP_011681684.1 class I SAM-dependent methyltransferase Machinery gene
  ST4134_RS08820 (ST4134_08790) comGG 1699768..1700085 (-) 318 WP_011681685.1 competence type IV pilus minor pilin ComGG -
  ST4134_RS08825 (ST4134_08795) comYF 1700063..1700500 (-) 438 WP_011681686.1 competence type IV pilus minor pilin ComGF Machinery gene
  ST4134_RS08830 (ST4134_08800) comGE 1700484..1700777 (-) 294 WP_024704238.1 competence type IV pilus minor pilin ComGE -
  ST4134_RS08835 (ST4134_08805) comYD 1700749..1701177 (-) 429 WP_011226626.1 competence type IV pilus minor pilin ComGD Machinery gene
  ST4134_RS08840 (ST4134_08810) comYC 1701137..1701463 (-) 327 WP_002946126.1 competence type IV pilus major pilin ComGC Machinery gene
  ST4134_RS08845 (ST4134_08815) comYB 1701460..1702560 (-) 1101 WP_120764773.1 competence type IV pilus assembly protein ComGB Machinery gene
  ST4134_RS08850 (ST4134_08820) comGA/cglA/cilD 1702442..1703383 (-) 942 WP_022096896.1 competence type IV pilus ATPase ComGA Machinery gene
  ST4134_RS08855 (ST4134_08825) - 1703464..1703826 (-) 363 WP_014622006.1 DUF1033 family protein -
  ST4134_RS08860 (ST4134_08830) rpoC 1703984..1707622 (-) 3639 WP_011681690.1 DNA-directed RNA polymerase subunit beta' -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35409.56 Da        Isoelectric Point: 6.3927

>NTDB_id=510094 ST4134_RS08850 WP_022096896.1 1702442..1703383(-) (comGA/cglA/cilD) [Streptococcus thermophilus strain 4134]
MVTEFAKEMIKNADSCGAQDIYVIPRQDNYELYMRVGQERRLIDVYRPDFMASLIGHFKFVAGMMVGEKRRSQLGSCDYD
CGDGHLVSLRLSTVGDYRGLESLVIRILHSERRELVYWNQGIQPIKDALDYRGLYLFAGPVGSGKTTLMHELVQERFKGQ
QVISIEDPVEIKQDNVLQLQVNQAIDMTYDSLIKLSLRHRPDVLIIGEIRDKETARAVIRASLTGVTVLSTIHAKSIAGV
YERLLDLGVDKSELDNALQGIAYMRLIKGGGVIDFARENFQNHSSTNWNQQLEGLVKQGCLTERDIQGEKIKD

Nucleotide


Download         Length: 942 bp        

>NTDB_id=510094 ST4134_RS08850 WP_022096896.1 1702442..1703383(-) (comGA/cglA/cilD) [Streptococcus thermophilus strain 4134]
ATGGTAACAGAATTTGCTAAGGAAATGATAAAAAATGCTGATAGTTGTGGGGCTCAAGACATCTATGTCATTCCACGTCA
GGATAATTATGAGCTTTATATGCGAGTCGGGCAAGAGAGGAGATTGATTGATGTATATCGGCCTGATTTCATGGCTAGTC
TTATTGGTCACTTTAAATTTGTGGCGGGAATGATGGTGGGTGAGAAGCGTAGGAGTCAACTGGGTTCATGTGACTATGAT
TGTGGTGACGGTCACTTGGTTTCTCTGCGTTTATCAACTGTTGGAGACTATCGAGGTTTGGAGAGCTTGGTTATCCGTAT
CCTACATTCTGAACGTCGGGAATTAGTGTATTGGAATCAAGGAATCCAGCCTATTAAGGATGCCTTGGATTATAGAGGAC
TGTATCTCTTTGCGGGTCCCGTAGGTTCTGGGAAGACCACACTTATGCATGAGTTGGTTCAGGAGCGTTTTAAGGGGCAG
CAGGTAATTTCGATTGAGGATCCTGTTGAAATCAAACAGGATAATGTCTTGCAACTCCAGGTTAATCAGGCGATTGATAT
GACCTATGATAGTTTGATTAAGCTATCTCTACGTCACCGTCCAGATGTTTTGATTATTGGGGAGATTCGGGATAAGGAGA
CTGCCCGGGCAGTTATTAGAGCTAGTCTAACAGGGGTAACTGTTCTTTCAACTATTCATGCTAAGAGTATTGCTGGCGTT
TACGAACGTCTCTTGGACCTTGGCGTAGATAAGTCTGAGTTGGATAATGCTCTACAAGGCATTGCCTACATGCGTTTGAT
CAAGGGAGGAGGTGTGATTGATTTTGCCAGAGAAAATTTCCAAAACCATTCGTCGACCAACTGGAATCAGCAGTTGGAAG
GTTTGGTTAAACAAGGATGTCTCACTGAGAGGGATATCCAAGGGGAAAAAATTAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

65.595

99.361

0.652
  comYA Streptococcus mutans UA159

64.856

100

0.649
  comYA Streptococcus mutans UA140

64.856

100

0.649
  comGA/cglA/cilD Streptococcus pneumoniae D39

64.309

99.361

0.639
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

64.309

99.361

0.639
  comGA/cglA/cilD Streptococcus pneumoniae R6

64.309

99.361

0.639
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

64.309

99.361

0.639
  comYA Streptococcus gordonii str. Challis substr. CH1

63.259

100

0.633
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

61.538

99.681

0.613
  comGA Lactococcus lactis subsp. cremoris KW2

51.125

99.361

0.508
  comGA Latilactobacillus sakei subsp. sakei 23K

38.816

97.125

0.377