Detailed information NTG0510090

Bioinformatically predicted

Overview

• Name: comYF
• Type: Machinery gene
• Locus tag: ST4134_RS08825
• Genome accession: NZ_CP065478
• Coordinates: 1700063..1700500 (-)
• Length: 145 a.a.
• NCBI ID: WP_011681686.1
• Uniprot ID: -
• Organism: Streptococcus thermophilus strain 4134
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1695063..1705500

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ST4134_RS08785 (ST4134_08760)	-	1695126..1695788 (-)	663	WP_011681679.1	CPBP family intramembrane glutamic endopeptidase	-
ST4134_RS08790 (ST4134_08765)	-	1695885..1696328 (-)	444	WP_011681680.1	hypothetical protein	-
ST4134_RS08795	-	1696402..1696740 (-)	339	WP_224103197.1	CPBP family intramembrane glutamic endopeptidase	-
ST4134_RS08800	-	1696784..1697059 (-)	276	WP_011681681.1	hypothetical protein	-
ST4134_RS08805 (ST4134_08775)	-	1697071..1697269 (-)	199	Protein_1677	helix-turn-helix transcriptional regulator	-
ST4134_RS08810 (ST4134_08780)	-	1697518..1698711 (-)	1194	WP_014608740.1	acetate kinase	-
ST4134_RS08815 (ST4134_08785)	comYH	1698767..1699723 (-)	957	WP_011681684.1	class I SAM-dependent methyltransferase	Machinery gene
ST4134_RS08820 (ST4134_08790)	comGG	1699768..1700085 (-)	318	WP_011681685.1	competence type IV pilus minor pilin ComGG	-
ST4134_RS08825 (ST4134_08795)	comYF	1700063..1700500 (-)	438	WP_011681686.1	competence type IV pilus minor pilin ComGF	Machinery gene
ST4134_RS08830 (ST4134_08800)	comGE	1700484..1700777 (-)	294	WP_024704238.1	competence type IV pilus minor pilin ComGE	-
ST4134_RS08835 (ST4134_08805)	comYD	1700749..1701177 (-)	429	WP_011226626.1	competence type IV pilus minor pilin ComGD	Machinery gene
ST4134_RS08840 (ST4134_08810)	comYC	1701137..1701463 (-)	327	WP_002946126.1	competence type IV pilus major pilin ComGC	Machinery gene
ST4134_RS08845 (ST4134_08815)	comYB	1701460..1702560 (-)	1101	WP_120764773.1	competence type IV pilus assembly protein ComGB	Machinery gene
ST4134_RS08850 (ST4134_08820)	comGA/cglA/cilD	1702442..1703383 (-)	942	WP_022096896.1	competence type IV pilus ATPase ComGA	Machinery gene
ST4134_RS08855 (ST4134_08825)	-	1703464..1703826 (-)	363	WP_014622006.1	DUF1033 family protein	-

Sequence

Protein

• Length: 145 a.a.
• Molecular weight: 16375.85 Da
• Isoelectric Point: 8.4500

>NTDB_id=510090 ST4134_RS08825 WP_011681686.1 1700063..1700500(-) (comYF) [Streptococcus thermophilus strain 4134]
MYLSSKLRAFTLLECLVALLVIAGSVQVYQGLTTVLVSNVKQVRQQENQDWLLFVQQMEAELEGCQLVKVEGNKLYVKQD
NQDLAFGLSSASDFRKTNADGRGYQPMLFDVKSSKVSQKNQIVTIQVTLKNELQRSFVYAFEKTG

Nucleotide

• Length: 438 bp

>NTDB_id=510090 ST4134_RS08825 WP_011681686.1 1700063..1700500(-) (comYF) [Streptococcus thermophilus strain 4134]
ATGTATCTAAGCAGTAAGCTGCGTGCCTTTACCCTCTTGGAATGTCTAGTGGCCTTGTTGGTCATTGCTGGCTCAGTTCA
AGTTTATCAAGGGTTAACCACTGTACTTGTGAGTAATGTTAAGCAGGTAAGACAACAAGAAAACCAAGATTGGCTTCTAT
TTGTTCAACAAATGGAGGCAGAATTAGAAGGATGTCAGTTGGTCAAGGTAGAGGGGAACAAACTCTATGTCAAGCAGGAT
AATCAAGACTTGGCTTTTGGTCTTTCAAGTGCCTCTGATTTTCGAAAAACAAATGCTGATGGTCGAGGTTATCAACCAAT
GCTTTTCGATGTCAAATCTAGTAAGGTAAGTCAAAAGAACCAGATAGTTACCATCCAAGTGACTCTTAAAAATGAACTAC
AAAGGAGTTTTGTTTATGCTTTTGAAAAAACAGGTTAG

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (39-134)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comYF	Streptococcus mutans UA140	58.156	97.241	0.566
comYF	Streptococcus mutans UA159	57.447	97.241	0.559
comGF/cglF	Streptococcus pneumoniae D39	49.286	96.552	0.476
comGF/cglF	Streptococcus pneumoniae Rx1	49.286	96.552	0.476
comGF/cglF	Streptococcus pneumoniae R6	49.286	96.552	0.476
comGF/cglF	Streptococcus pneumoniae TIGR4	49.286	96.552	0.476
comGF/cglF	Streptococcus mitis NCTC 12261	47.518	97.241	0.462
comGF/cglF	Streptococcus mitis SK321	46.809	97.241	0.455
comGF	Lactococcus lactis subsp. cremoris KW2	45.324	95.862	0.434