Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ITG08_RS04550 Genome accession   NZ_CP065206
Coordinates   970138..971829 (-) Length   563 a.a.
NCBI ID   WP_248370686.1    Uniprot ID   -
Organism   Vibrio cyclitrophicus strain ED653     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 965138..976829
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ITG08_RS04530 (ITG08_04520) - 965817..966662 (-) 846 WP_010435266.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  ITG08_RS04535 (ITG08_04525) - 966732..968249 (-) 1518 WP_248370547.1 AAA family ATPase -
  ITG08_RS04540 (ITG08_04530) aroB 968330..969418 (-) 1089 WP_010435273.1 3-dehydroquinate synthase -
  ITG08_RS04545 (ITG08_04535) aroK 969443..969961 (-) 519 WP_009848907.1 shikimate kinase AroK -
  ITG08_RS04550 (ITG08_04540) pilQ 970138..971829 (-) 1692 WP_248370686.1 type IV pilus secretin PilQ family protein Machinery gene
  ITG08_RS04555 (ITG08_04545) pilP 971889..972407 (-) 519 WP_016787693.1 pilus assembly protein PilP Machinery gene
  ITG08_RS04560 (ITG08_04550) pilO 972394..973023 (-) 630 WP_010435284.1 type 4a pilus biogenesis protein PilO Machinery gene
  ITG08_RS04565 (ITG08_04555) pilN 973016..973630 (-) 615 WP_010435288.1 PilN domain-containing protein Machinery gene
  ITG08_RS04570 (ITG08_04560) pilM 973614..974639 (-) 1026 WP_016787692.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 563 a.a.        Molecular weight: 61433.97 Da        Isoelectric Point: 4.3767

>NTDB_id=508008 ITG08_RS04550 WP_248370686.1 970138..971829(-) (pilQ) [Vibrio cyclitrophicus strain ED653]
MNRGLQGIVLSVVLFFTVMSHAETSNKLENIDFRVNKDKEAVIIIELATTTAVVDVQRVQEGLSIELINTQVDDDKLYLL
DVKDFATLVEGIEVFRELPSTRLLANISNDYQYEYNLKGRFIEVVVSQPKINEAAIEKSILEKEGKLISINFQDIPVRNV
LQLIADYNDFNLVVSDSVAGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKAELDLREQQALEKSRLEEELGE
LKSEIIKINFAKATDIADMIGGEGAVSMLSDRGSITIDERTNSMLIRELEENIAVIRGIIESLDIPVKQVQIEARIVTVT
EGNLDELGVRWGVSSNNGSFTVGGSIEGNHPSQITPYDDNGGNSAIDDYLNVNLGATSPNASSIAFQVAKLGSDTLLDLE
LSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATSVAFKKAVLSLKVTPQITPDNRLVLDLSVTQDRPG
QVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSVSSTVDKVPLLGDLPVLGALFRRSYENVGKSELLIFVTPKV
VIQ

Nucleotide


Download         Length: 1692 bp        

>NTDB_id=508008 ITG08_RS04550 WP_248370686.1 970138..971829(-) (pilQ) [Vibrio cyclitrophicus strain ED653]
ATGAACAGAGGGCTGCAAGGCATTGTGTTGTCGGTGGTGTTGTTTTTTACGGTGATGAGTCATGCAGAGACCTCCAACAA
GCTGGAGAATATTGATTTCAGGGTCAATAAGGACAAAGAAGCGGTCATCATTATTGAACTGGCAACGACTACTGCGGTTG
TCGATGTTCAGCGAGTGCAAGAAGGGTTGAGTATTGAACTGATCAATACTCAGGTTGACGACGATAAACTGTACCTACTG
GACGTAAAGGACTTTGCGACTTTAGTTGAGGGGATTGAAGTCTTTAGGGAGTTGCCGAGCACTCGGCTGTTGGCAAACAT
CTCCAATGACTACCAATATGAGTACAACCTCAAAGGACGTTTTATTGAGGTTGTTGTGAGTCAGCCTAAGATCAATGAAG
CTGCGATTGAGAAAAGTATCTTAGAAAAAGAGGGCAAACTGATATCGATTAACTTCCAAGATATCCCGGTACGAAATGTC
TTGCAGCTGATTGCCGATTACAATGATTTCAATCTAGTCGTATCTGATTCGGTGGCAGGTAACCTAACTTTACGTTTAGA
TGGCGTACCTTGGCAGCAAGTTCTCGATATTATCTTGCAAGTTAAAGGCTTAGATAAACGTGTTGATGGCAACGTTATCT
TGGTCGCACCGAAAGCGGAACTCGATCTTCGAGAGCAGCAAGCGCTCGAGAAATCGCGTTTAGAAGAGGAGTTGGGTGAA
CTCAAATCTGAAATCATCAAAATCAACTTTGCTAAAGCGACCGACATTGCCGACATGATTGGCGGTGAAGGTGCGGTGAG
TATGTTGTCTGATCGAGGTTCAATTACCATTGATGAACGAACCAACTCAATGCTCATTCGTGAGTTGGAAGAAAACATTG
CGGTGATCCGAGGCATCATTGAATCGCTAGATATTCCGGTGAAGCAAGTTCAGATAGAGGCGCGCATCGTAACTGTCACC
GAGGGTAACCTGGATGAGCTTGGTGTGCGCTGGGGCGTTTCCTCAAACAACGGAAGCTTCACCGTTGGCGGTTCAATTGA
AGGCAACCACCCATCGCAAATTACGCCGTATGACGATAATGGTGGGAATAGTGCGATTGATGATTATCTCAACGTCAATT
TAGGGGCAACATCGCCGAACGCATCGAGTATTGCGTTTCAGGTTGCTAAGTTGGGTTCGGATACGCTGCTCGATCTTGAA
TTATCAGCGCTACAACAAGAATCTAAAGCCGAAATAATTTCGAGCCCACGCTTAATCACCACCAATAAAAAACCGGCTTA
TATTGAGCAAGGTACGGAAATTCCTTATTTGGAGTCCTCTTCAAGTGGTGCAACGTCGGTCGCATTTAAGAAAGCGGTAT
TAAGTTTGAAGGTGACACCGCAGATCACGCCAGATAACCGCTTAGTGTTGGACTTGAGTGTGACTCAAGATAGACCTGGG
CAGGTAGTGAAAACAGGAACGGGCGAAGCAGTGGCCATTGATACTCAACGAATAGGAACGCAAGTGCTTGTTAATAATGG
TGAAACAGTCGTACTTGGCGGGATTTTTCAACATAGTGTCAGCAGTACTGTCGACAAAGTTCCGCTGTTGGGCGACCTGC
CAGTTTTAGGCGCATTGTTCCGTCGCAGCTATGAAAATGTAGGCAAAAGTGAACTGCTTATTTTTGTTACACCTAAAGTT
GTGATTCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(244-309)

Bacterial type II and III secretion system protein (Secretin)

(403-561)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

81.284

96.803

0.787
  pilQ Vibrio cholerae strain A1552

79.779

96.625

0.771
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

79.779

96.625

0.771
  pilQ Legionella pneumophila strain ERS1305867

42.857

96.98

0.416
  pilQ Legionella pneumophila strain Lp02

42.857

96.98

0.416
  pilQ Pseudomonas aeruginosa PAK

40.813

100

0.41
  pilQ Acinetobacter baumannii strain A118

37.167

100

0.396
  pilQ Acinetobacter baumannii D1279779

37.167

100

0.396
  comQ Acinetobacter baylyi ADP1

39.223

100

0.394