Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   ITG08_RS04570 Genome accession   NZ_CP065206
Coordinates   973614..974639 (-) Length   341 a.a.
NCBI ID   WP_016787692.1    Uniprot ID   A0AAN0LM26
Organism   Vibrio cyclitrophicus strain ED653     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 968614..979639
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ITG08_RS04545 (ITG08_04535) aroK 969443..969961 (-) 519 WP_009848907.1 shikimate kinase AroK -
  ITG08_RS04550 (ITG08_04540) pilQ 970138..971829 (-) 1692 WP_248370686.1 type IV pilus secretin PilQ family protein Machinery gene
  ITG08_RS04555 (ITG08_04545) pilP 971889..972407 (-) 519 WP_016787693.1 pilus assembly protein PilP Machinery gene
  ITG08_RS04560 (ITG08_04550) pilO 972394..973023 (-) 630 WP_010435284.1 type 4a pilus biogenesis protein PilO Machinery gene
  ITG08_RS04565 (ITG08_04555) pilN 973016..973630 (-) 615 WP_010435288.1 PilN domain-containing protein Machinery gene
  ITG08_RS04570 (ITG08_04560) pilM 973614..974639 (-) 1026 WP_016787692.1 type IV pilus assembly protein PilM Machinery gene
  ITG08_RS04575 (ITG08_04565) - 974791..977349 (+) 2559 WP_010435294.1 PBP1A family penicillin-binding protein -
  ITG08_RS04580 (ITG08_04570) oxyR 977445..978338 (-) 894 WP_010435297.1 DNA-binding transcriptional regulator OxyR -
  ITG08_RS04585 (ITG08_04575) - 978560..979288 (+) 729 WP_010435300.1 glutathione peroxidase -

Sequence

Protein


Download         Length: 341 a.a.        Molecular weight: 38128.77 Da        Isoelectric Point: 6.8348

>NTDB_id=508012 ITG08_RS04570 WP_016787692.1 973614..974639(-) (pilM) [Vibrio cyclitrophicus strain ED653]
MGSSLITGIDINHHSIKAVVLKPVGESYALVGYKELPISDDIFTANHTLEYQKTVKKLKELRKGLPFGSRNVAISVPDNT
VISKVLQIESELEDREKEFSIYQTFAHQSPFPIEELSLDFVKLEDKRFGKGSTSSYQVYATRKEVVDSRADALTKAGFKP
IVVDTQAHGLLNIWQLASHLYPEKKNWLLVDVGVDQTSLGIIPQGSAPFYKDIAFGIQDLCSTGNSMDMEGGFSNVEEKH
KFIVNLIEKLKRQLQLYSSVNALQPISGIWLMGEGASMPMVVEELERHFQLSCESLNPLSLFENKVAKKRRLPMDWQHFG
IAAGMAMSGLKWQGEKHVASN

Nucleotide


Download         Length: 1026 bp        

>NTDB_id=508012 ITG08_RS04570 WP_016787692.1 973614..974639(-) (pilM) [Vibrio cyclitrophicus strain ED653]
ATGGGTTCATCATTAATTACAGGTATAGATATAAATCACCACAGCATCAAAGCCGTGGTACTAAAACCCGTTGGGGAGTC
GTATGCCCTGGTGGGATACAAAGAGCTGCCGATTTCGGACGACATTTTTACAGCTAACCATACTTTGGAGTATCAGAAAA
CTGTTAAGAAACTCAAAGAACTTAGGAAAGGGCTGCCTTTTGGAAGCCGCAACGTCGCTATTTCGGTACCGGATAACACG
GTGATCAGCAAAGTACTGCAAATAGAGAGCGAATTAGAAGATAGAGAAAAGGAGTTCTCGATCTATCAAACCTTTGCTCA
TCAATCTCCCTTTCCTATCGAGGAGCTGAGTTTAGATTTTGTAAAGCTAGAAGACAAACGATTTGGTAAAGGATCAACAA
GCAGCTATCAAGTGTATGCGACTCGTAAAGAGGTGGTTGATAGCCGAGCTGACGCGCTAACGAAAGCAGGGTTTAAGCCC
ATCGTTGTCGACACTCAGGCCCATGGTTTACTCAACATTTGGCAATTGGCTTCGCATTTGTATCCAGAAAAAAAGAATTG
GCTGTTGGTGGATGTTGGCGTTGATCAAACTTCATTAGGCATTATTCCCCAAGGTTCAGCGCCATTTTATAAAGATATTG
CCTTTGGCATTCAAGATCTTTGCAGTACGGGTAACTCGATGGATATGGAGGGTGGGTTTAGTAACGTTGAGGAGAAGCAT
AAGTTTATTGTCAATTTGATTGAAAAACTCAAGCGTCAGTTACAGCTCTATTCATCGGTCAATGCGCTTCAACCTATCTC
AGGAATTTGGCTAATGGGCGAAGGAGCAAGTATGCCGATGGTCGTTGAAGAGTTAGAGCGACATTTTCAGTTAAGTTGTG
AGTCTTTGAACCCCCTATCTTTGTTTGAGAATAAGGTCGCGAAAAAACGTCGATTACCCATGGATTGGCAACATTTTGGT
ATTGCAGCAGGCATGGCGATGAGTGGACTCAAGTGGCAGGGAGAGAAGCATGTTGCATCAAATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-220)

Type IV pilus assembly protein PilM; (PilM_2)

(242-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

53.392

99.413

0.531
  pilM Vibrio campbellii strain DS40M4

47.661

100

0.478