Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   ITG08_RS04560 Genome accession   NZ_CP065206
Coordinates   972394..973023 (-) Length   209 a.a.
NCBI ID   WP_010435284.1    Uniprot ID   A0AAN0LMD0
Organism   Vibrio cyclitrophicus strain ED653     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 967394..978023
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ITG08_RS04540 (ITG08_04530) aroB 968330..969418 (-) 1089 WP_010435273.1 3-dehydroquinate synthase -
  ITG08_RS04545 (ITG08_04535) aroK 969443..969961 (-) 519 WP_009848907.1 shikimate kinase AroK -
  ITG08_RS04550 (ITG08_04540) pilQ 970138..971829 (-) 1692 WP_248370686.1 type IV pilus secretin PilQ family protein Machinery gene
  ITG08_RS04555 (ITG08_04545) pilP 971889..972407 (-) 519 WP_016787693.1 pilus assembly protein PilP Machinery gene
  ITG08_RS04560 (ITG08_04550) pilO 972394..973023 (-) 630 WP_010435284.1 type 4a pilus biogenesis protein PilO Machinery gene
  ITG08_RS04565 (ITG08_04555) pilN 973016..973630 (-) 615 WP_010435288.1 PilN domain-containing protein Machinery gene
  ITG08_RS04570 (ITG08_04560) pilM 973614..974639 (-) 1026 WP_016787692.1 type IV pilus assembly protein PilM Machinery gene
  ITG08_RS04575 (ITG08_04565) - 974791..977349 (+) 2559 WP_010435294.1 PBP1A family penicillin-binding protein -

Sequence

Protein


Download         Length: 209 a.a.        Molecular weight: 24324.99 Da        Isoelectric Point: 4.9074

>NTDB_id=508010 ITG08_RS04560 WP_010435284.1 972394..973023(-) (pilO) [Vibrio cyclitrophicus strain ED653]
MANLQNRISWQNRMSLQDLDVDEITEWPLLPQLLVILLLMVLIQGLGTWLYILPLDDELQKMKQQEQTLKATLRIKANKV
AALPKLQSQLDELTSRYDYLLEQLPVQKELASMLASVNELGLDNKLTFTRIDWGQKQNKAFLYRLPLNIELTGDYHEIGD
FSAAIAKLPRIISFDDVNWQRVSQESSTLHFRVRAYTYQFKSEVNDETQ

Nucleotide


Download         Length: 630 bp        

>NTDB_id=508010 ITG08_RS04560 WP_010435284.1 972394..973023(-) (pilO) [Vibrio cyclitrophicus strain ED653]
ATGGCTAACCTTCAAAATAGGATTAGTTGGCAAAATAGGATGAGCTTGCAAGATCTTGATGTTGATGAAATCACCGAATG
GCCGTTACTGCCTCAATTACTGGTGATTCTGTTGTTGATGGTGCTTATCCAAGGATTAGGGACTTGGCTCTATATTCTGC
CTTTGGACGATGAGCTACAGAAAATGAAACAACAAGAGCAAACCTTAAAAGCGACCTTGAGAATAAAGGCCAATAAGGTT
GCAGCCTTGCCTAAGTTGCAGAGTCAGTTAGATGAATTAACTAGCCGTTACGACTACTTGTTAGAGCAACTACCGGTTCA
AAAGGAGCTGGCCAGCATGTTGGCCTCAGTGAATGAACTTGGCTTGGATAACAAGCTGACGTTTACACGGATTGATTGGG
GGCAGAAGCAAAACAAAGCGTTTCTGTATCGGTTACCTCTCAATATCGAGTTGACGGGTGACTACCATGAGATTGGTGAT
TTCTCGGCGGCCATCGCTAAGCTGCCACGAATTATTAGCTTTGATGATGTGAACTGGCAGCGAGTCAGTCAAGAAAGTAG
CACGCTGCACTTTAGGGTTCGCGCTTATACCTACCAGTTTAAGTCGGAGGTGAATGATGAAACCCAGTAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(59-201)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

76.684

92.344

0.708
  pilO Vibrio campbellii strain DS40M4

74.054

88.517

0.656