Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   ITG08_RS04565 Genome accession   NZ_CP065206
Coordinates   973016..973630 (-) Length   204 a.a.
NCBI ID   WP_010435288.1    Uniprot ID   -
Organism   Vibrio cyclitrophicus strain ED653     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 968016..978630
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ITG08_RS04540 (ITG08_04530) aroB 968330..969418 (-) 1089 WP_010435273.1 3-dehydroquinate synthase -
  ITG08_RS04545 (ITG08_04535) aroK 969443..969961 (-) 519 WP_009848907.1 shikimate kinase AroK -
  ITG08_RS04550 (ITG08_04540) pilQ 970138..971829 (-) 1692 WP_248370686.1 type IV pilus secretin PilQ family protein Machinery gene
  ITG08_RS04555 (ITG08_04545) pilP 971889..972407 (-) 519 WP_016787693.1 pilus assembly protein PilP Machinery gene
  ITG08_RS04560 (ITG08_04550) pilO 972394..973023 (-) 630 WP_010435284.1 type 4a pilus biogenesis protein PilO Machinery gene
  ITG08_RS04565 (ITG08_04555) pilN 973016..973630 (-) 615 WP_010435288.1 PilN domain-containing protein Machinery gene
  ITG08_RS04570 (ITG08_04560) pilM 973614..974639 (-) 1026 WP_016787692.1 type IV pilus assembly protein PilM Machinery gene
  ITG08_RS04575 (ITG08_04565) - 974791..977349 (+) 2559 WP_010435294.1 PBP1A family penicillin-binding protein -
  ITG08_RS04580 (ITG08_04570) oxyR 977445..978338 (-) 894 WP_010435297.1 DNA-binding transcriptional regulator OxyR -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 23345.82 Da        Isoelectric Point: 9.3072

>NTDB_id=508011 ITG08_RS04565 WP_010435288.1 973016..973630(-) (pilN) [Vibrio cyclitrophicus strain ED653]
MLHQINLLSWRDEIRAQHKKRFIHLVILGVIIALAGQWAVGHYFHDQQAKQQARLSYLDKYIAVLDRQIQSLKVAEQEHK
AILTRLAVVESLQFGRNKTTDFMNLMPELIPEGVYVDKIKMNGQEIEMSGISDSTARLATMLDNLERSDSLKEVEMHSIV
HNRKRFNKEFQSFKVSFVFSPVSLENTTAEGTTANIKEKGANHG

Nucleotide


Download         Length: 615 bp        

>NTDB_id=508011 ITG08_RS04565 WP_010435288.1 973016..973630(-) (pilN) [Vibrio cyclitrophicus strain ED653]
ATGTTGCATCAAATTAACCTGTTGTCGTGGCGTGATGAGATTCGCGCTCAGCATAAAAAGCGTTTCATTCATCTGGTCAT
TCTTGGCGTCATCATTGCGCTTGCCGGGCAATGGGCGGTGGGACATTATTTTCATGACCAACAAGCCAAGCAGCAAGCGC
GCCTCAGTTATCTCGATAAGTACATCGCAGTGCTCGACCGACAAATCCAGTCATTAAAAGTCGCAGAGCAAGAGCACAAA
GCCATCCTTACACGACTTGCTGTCGTGGAGTCTTTACAGTTCGGGCGCAATAAAACCACCGACTTTATGAACCTGATGCC
GGAGCTGATTCCTGAGGGCGTGTATGTCGACAAGATAAAGATGAATGGTCAGGAGATTGAAATGTCGGGCATCAGCGACA
GCACCGCTCGTCTAGCCACCATGTTGGACAACCTAGAACGTTCTGACTCTCTTAAAGAAGTTGAGATGCACTCTATTGTT
CATAACCGTAAGCGCTTCAATAAAGAGTTTCAGAGCTTCAAGGTCTCTTTTGTGTTTAGCCCTGTTTCTTTAGAAAACAC
AACCGCAGAGGGCACAACTGCGAACATAAAAGAGAAGGGGGCGAATCATGGCTAA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(103-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio cholerae strain A1552

63.731

94.608

0.603
  pilN Vibrio campbellii strain DS40M4

61.78

93.627

0.578