Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   INH39_RS33665 Genome accession   NZ_CP063361
Coordinates   1664622..1665056 (-) Length   144 a.a.
NCBI ID   WP_279401146.1    Uniprot ID   -
Organism   Massilia violaceinigra strain sipir     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1659622..1670056
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INH39_RS07525 (INH39_07485) - 1660021..1661613 (-) 1593 WP_243492653.1 M48 family metalloprotease -
  INH39_RS07530 (INH39_07490) moaC 1661704..1662204 (+) 501 WP_243492654.1 cyclic pyranopterin monophosphate synthase MoaC -
  INH39_RS07535 (INH39_07495) - 1662236..1662688 (-) 453 WP_279401144.1 pilin -
  INH39_RS33660 (INH39_07500) - 1662972..1663514 (-) 543 WP_279401145.1 pilin -
  INH39_RS07555 (INH39_07505) - 1663749..1664192 (-) 444 WP_370663035.1 pilin -
  INH39_RS33665 (INH39_07510) pilA/pilAI 1664622..1665056 (-) 435 WP_279401146.1 pilin Machinery gene
  INH39_RS07575 (INH39_07515) - 1665283..1665876 (-) 594 WP_243492655.1 FHA domain-containing protein -
  INH39_RS07580 (INH39_07520) sucD 1665979..1666860 (-) 882 WP_099874964.1 succinate--CoA ligase subunit alpha -
  INH39_RS07585 (INH39_07525) sucC 1666876..1668048 (-) 1173 WP_167073468.1 ADP-forming succinate--CoA ligase subunit beta -
  INH39_RS07590 (INH39_07530) - 1668122..1668688 (-) 567 WP_243492656.1 DUF2889 domain-containing protein -
  INH39_RS07595 (INH39_07535) - 1668990..1669295 (-) 306 WP_243492657.1 hypothetical protein -
  INH39_RS07600 (INH39_07540) - 1669380..1670054 (-) 675 WP_243492658.1 hypothetical protein -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15204.74 Da        Isoelectric Point: 6.9004

>NTDB_id=494748 INH39_RS33665 WP_279401146.1 1664622..1665056(-) (pilA/pilAI) [Massilia violaceinigra strain sipir]
MRTLKNVQRGFTLIELMIVVAIIGILASIALPAYQEYVSRTQAVTGLAELTAGKVIVEMKLAEGITTALTNPEEIGLQSV
TSRCDIAISIVADGDTTLICTLKGNANAMGSIITLTRTADSAVTAGHWTCTSNLPARVKPKDCQ

Nucleotide


Download         Length: 435 bp        

>NTDB_id=494748 INH39_RS33665 WP_279401146.1 1664622..1665056(-) (pilA/pilAI) [Massilia violaceinigra strain sipir]
ATGCGCACGCTGAAAAACGTTCAGCGCGGTTTCACACTGATCGAGCTGATGATCGTCGTGGCAATTATTGGCATCCTGGC
CAGCATCGCTTTGCCTGCGTATCAAGAGTATGTATCTAGGACGCAAGCTGTGACGGGACTGGCCGAACTCACCGCTGGAA
AAGTCATTGTGGAAATGAAGCTGGCGGAGGGGATCACCACGGCCCTGACCAACCCCGAAGAAATTGGCTTGCAATCGGTC
ACTAGCCGCTGTGATATCGCTATATCCATTGTGGCCGATGGGGACACGACACTGATATGTACACTGAAGGGTAACGCCAA
CGCGATGGGATCGATCATCACGCTCACAAGGACAGCTGACAGCGCTGTTACCGCAGGACACTGGACATGTACGTCTAACT
TGCCTGCCAGGGTCAAACCCAAAGACTGCCAGTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(6-31)

Pilin (bacterial filament) (Pilin)

(39-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

52.482

97.917

0.514
  pilA/pilAII Pseudomonas stutzeri DSM 10701

45.333

100

0.472
  pilA Acinetobacter baumannii strain A118

44.056

99.306

0.438
  pilA Vibrio cholerae C6706

41.06

100

0.431
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

41.06

100

0.431
  pilA Vibrio cholerae strain A1552

41.06

100

0.431
  pilA Pseudomonas aeruginosa PAK

38.462

100

0.417
  pilA2 Legionella pneumophila str. Paris

40.816

100

0.417
  pilA2 Legionella pneumophila strain ERS1305867

40.816

100

0.417
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

29.787

100

0.389
  comP Acinetobacter baylyi ADP1

36.601

100

0.389