Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   INR79_RS02450 Genome accession   NZ_CP063199
Coordinates   513368..513778 (-) Length   136 a.a.
NCBI ID   WP_262415375.1    Uniprot ID   -
Organism   Vibrio sp. SCSIO 43132     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 508368..518778
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INR79_RS02430 (INR79_02430) coaE 508864..509469 (-) 606 WP_022599625.1 dephospho-CoA kinase -
  INR79_RS02435 (INR79_02435) pilD 509519..510388 (-) 870 WP_045966127.1 prepilin peptidase Machinery gene
  INR79_RS02440 (INR79_02440) pilC 510429..511661 (-) 1233 WP_223095884.1 type II secretion system F family protein Machinery gene
  INR79_RS02445 (INR79_02445) pilB 511671..513359 (-) 1689 WP_223095887.1 type IV-A pilus assembly ATPase PilB Machinery gene
  INR79_RS02450 (INR79_02450) pilA 513368..513778 (-) 411 WP_262415375.1 pilin Machinery gene
  INR79_RS02455 (INR79_02455) nadC 514056..514943 (-) 888 WP_223095892.1 carboxylating nicotinate-nucleotide diphosphorylase -
  INR79_RS02460 (INR79_02460) ampD 515072..515629 (+) 558 WP_223095894.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  INR79_RS02465 (INR79_02465) pdhR 515942..516712 (+) 771 WP_004397664.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14065.29 Da        Isoelectric Point: 9.5423

>NTDB_id=493802 INR79_RS02450 WP_262415375.1 513368..513778(-) (pilA) [Vibrio sp. SCSIO 43132]
MKNKKQQGFTLIELMIVVAVIGVLAAIAVPQYQKYVGKSEAATALASITGLRTNVETYVVEMGAFPTISQVVPPSSALGT
ITFPNAASAAGDIKFEFKSTGVSPSVVGKHVTLVRSSSGGWSCSTDVGSDIKPKGC

Nucleotide


Download         Length: 411 bp        

>NTDB_id=493802 INR79_RS02450 WP_262415375.1 513368..513778(-) (pilA) [Vibrio sp. SCSIO 43132]
ATGAAAAATAAAAAACAACAGGGCTTTACGCTGATAGAGCTAATGATTGTTGTGGCGGTAATCGGAGTTTTAGCGGCAAT
TGCTGTACCTCAATATCAAAAATATGTAGGGAAGTCAGAAGCAGCAACAGCTTTGGCTTCCATCACTGGCCTAAGAACAA
ATGTTGAAACTTATGTGGTAGAAATGGGAGCTTTCCCTACAATATCCCAAGTAGTTCCACCATCTTCTGCTTTGGGCACT
ATTACCTTCCCTAATGCTGCGAGTGCAGCTGGTGATATCAAATTTGAGTTTAAAAGTACTGGTGTTAGCCCTTCTGTTGT
AGGTAAACATGTAACACTGGTTCGCTCTAGCTCTGGAGGCTGGTCATGCTCTACTGACGTTGGTTCTGACATTAAACCCA
AGGGCTGTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(3-28)

Pilin (bacterial filament) (Pilin)

(38-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae strain A1552

50

100

0.537
  pilA Vibrio cholerae C6706

50

100

0.537
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

50

100

0.537
  pilA Pseudomonas aeruginosa PAK

44.218

100

0.478
  pilA Acinetobacter baumannii strain A118

43.796

100

0.441
  pilA Vibrio parahaemolyticus RIMD 2210633

45.312

94.118

0.426
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40.141

100

0.419
  pilA/pilAII Pseudomonas stutzeri DSM 10701

40.441

100

0.404